##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547576_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2152285
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.480239373503043	31.0	31.0	33.0	30.0	34.0
2	31.910563424453546	31.0	31.0	34.0	30.0	34.0
3	32.05892435249049	33.0	31.0	34.0	30.0	34.0
4	35.77676887586914	37.0	35.0	37.0	35.0	37.0
5	35.69425656918113	37.0	35.0	37.0	33.0	37.0
6	35.7362886420711	37.0	35.0	37.0	33.0	37.0
7	36.06152902612804	37.0	35.0	37.0	35.0	37.0
8	36.01869733794548	37.0	35.0	37.0	35.0	37.0
9	37.680641271950506	39.0	38.0	39.0	35.0	39.0
10	37.236997423668335	39.0	37.0	39.0	34.0	39.0
11	37.00288298250464	39.0	37.0	39.0	33.0	39.0
12	36.73534964003373	39.0	35.0	39.0	33.0	39.0
13	36.630750574389545	39.0	35.0	39.0	33.0	39.0
14	37.649895808408274	40.0	36.0	41.0	32.0	41.0
15	37.85870551530118	40.0	36.0	41.0	33.0	41.0
16	37.949088062222245	40.0	36.0	41.0	33.0	41.0
17	37.89684776876668	40.0	36.0	41.0	33.0	41.0
18	37.81711158141231	40.0	36.0	41.0	33.0	41.0
19	37.807523631861024	40.0	36.0	41.0	33.0	41.0
20	37.705418195081045	40.0	36.0	41.0	33.0	41.0
21	37.6058858376098	39.0	35.0	41.0	33.0	41.0
22	37.561959034235706	39.0	35.0	41.0	33.0	41.0
23	37.49150879181893	39.0	35.0	41.0	33.0	41.0
24	37.45692508194779	39.0	35.0	41.0	32.0	41.0
25	37.377901160859274	39.0	35.0	41.0	32.0	41.0
26	37.41090422504455	39.0	35.0	41.0	33.0	41.0
27	37.4000775919546	39.0	35.0	41.0	32.0	41.0
28	37.35659357380644	39.0	35.0	41.0	32.0	41.0
29	37.3219378474505	39.0	35.0	41.0	32.0	41.0
30	37.22235298763872	39.0	35.0	41.0	32.0	41.0
31	37.11507955498459	39.0	35.0	41.0	32.0	41.0
32	37.029140657487275	39.0	35.0	41.0	31.0	41.0
33	36.94243141591379	39.0	35.0	41.0	31.0	41.0
34	36.871938892851084	39.0	35.0	41.0	31.0	41.0
35	36.74555600210939	39.0	35.0	41.0	31.0	41.0
36	36.64420325375124	39.0	35.0	41.0	30.0	41.0
37	36.600924598740406	39.0	35.0	41.0	30.0	41.0
38	36.432221104547025	39.0	35.0	41.0	30.0	41.0
39	36.24462606021043	39.0	35.0	41.0	30.0	41.0
40	36.07882831502334	39.0	35.0	41.0	29.0	41.0
41	35.96283205988055	39.0	35.0	41.0	28.0	41.0
42	35.831625923146795	39.0	35.0	41.0	27.0	41.0
43	35.75445723963137	39.0	35.0	41.0	27.0	41.0
44	35.577761309492004	38.0	35.0	41.0	26.0	41.0
45	35.38229881265725	38.0	35.0	40.0	25.0	41.0
46	35.22696761813607	38.0	34.0	40.0	24.0	41.0
47	35.12940340150119	38.0	34.0	40.0	24.0	41.0
48	35.05631782036301	38.0	34.0	40.0	24.0	41.0
49	34.937562172295955	38.0	34.0	40.0	24.0	41.0
50	34.79456298770841	38.0	34.0	40.0	23.0	41.0
51	34.319005614962705	37.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	9.0
11	6.0
12	12.0
13	14.0
14	23.0
15	44.0
16	82.0
17	250.0
18	480.0
19	927.0
20	1680.0
21	2795.0
22	4568.0
23	7120.0
24	11013.0
25	16571.0
26	22816.0
27	27417.0
28	31939.0
29	38074.0
30	46762.0
31	59426.0
32	74956.0
33	96799.0
34	150182.0
35	190280.0
36	158855.0
37	206146.0
38	329567.0
39	673272.0
40	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.17938144808889	25.14239517536014	30.34198537833047	12.336237998220497
2	30.447965766615482	25.61742520158808	30.821940402874155	13.112668628922286
3	29.803441458728745	25.029259600842824	31.51511068469092	13.652188255737506
4	27.205644233918836	27.180182921871403	31.284379159823168	14.329793684386594
5	24.20627379738278	32.55010372696924	29.20844590748902	14.035176568158958
6	23.29394109051543	39.13937977544795	26.956606583235953	10.61007255080066
7	88.12127576041277	3.6275864952829204	6.32490585586946	1.9262318884348495
8	88.07625384184715	3.33208659633831	6.1447252571104665	2.44693430470407
9	82.26759002641379	6.413555825552843	8.021196077657002	3.2976580703763676
10	39.435019061137346	34.585661285563944	15.204073809927587	10.775245843371115
11	28.90342124765075	25.884861902582607	27.732154431220774	17.47956241854587
12	28.14808447765979	22.58134958892526	30.501629663357782	18.76893627005717
13	26.248010834996293	24.480401062126994	30.511990744720148	18.75959735815656
14	18.843368791772466	29.16881360972176	32.12878405973187	19.859033538773907
15	17.461163368234224	30.96434719379636	34.28951091514367	17.284978522825742
16	23.80581567961492	29.000852582255604	31.06661060222043	16.126721135909044
17	24.07255544688552	29.422125787244717	26.922317444018802	19.58300132185096
18	22.82773889145722	29.383051036456603	30.811439934767005	16.977770137319176
19	24.256824723491548	30.798848665488077	27.253686198621462	17.690640412398917
20	24.417351791235824	31.17235867926413	28.140696980186174	16.26959254931387
21	24.719217018192293	27.922138564362992	31.775206350460095	15.583438066984623
22	23.0864871520268	23.412141050093275	32.991727396697	20.50964440118293
23	20.353113086789158	27.254243745600604	32.244242746662266	20.148400420947972
24	19.669839263852136	28.162720085862237	33.67820711476408	18.489233535521553
25	20.80430797965883	30.095224377812418	29.240086698555256	19.8603809439735
26	19.43506552338561	32.658918312398214	27.56702760089858	20.33898856331759
27	18.862743549297605	31.824224022376217	30.684040450033333	18.628991978292838
28	17.675865417451686	30.272756628420495	32.50289808273533	19.548479871392495
29	19.305203539494073	28.477501817835467	32.26923943622708	19.948055206443385
30	21.02314516897158	28.710509992868044	31.12835893016027	19.1379859080001
31	21.725561438192432	28.24500472753376	30.010802472720854	20.018631361552956
32	23.412048125596748	28.25764245906095	29.701038663559892	18.62927075178241
33	22.758370754802453	27.8724704209712	29.545157820641784	19.824001003584563
34	20.042791730649054	27.44362386951542	31.658539645074885	20.855044754760637
35	20.758310353879715	28.572098955296347	30.53568649133363	20.13390419949031
36	22.150226387304656	28.431736503297657	30.1951646738234	19.222872435574285
37	20.864662440150816	29.963875601976504	29.579818657845035	19.591643300027645
38	21.566242388902957	29.510218209948963	28.52628717851028	20.397252222637803
39	20.26520651307796	28.330820500073177	29.334776760512664	22.069196226336196
40	22.33365934344197	27.53938256318285	29.786436275864954	20.340521817510226
41	19.717881228554766	26.413509363304584	30.09545668905373	23.773152719086923
42	19.961157560453195	26.998794304657608	30.846286620963305	22.193761513925896
43	20.441809518720802	26.837152142955045	30.41116766599219	22.309870672331964
44	20.2984270205851	28.423280374114025	29.91225604415772	21.366036561143158
45	19.77056941808357	28.949465335678127	29.45539275700012	21.824572489238182
46	21.60122846184404	28.473924224719312	28.84246277793136	21.082384535505287
47	19.63480672866279	27.56029057490063	31.00323609559143	21.80166660084515
48	19.59043528157284	28.11788401628966	31.57179462757023	20.719886074567263
49	21.317390587213126	26.807462766315798	30.838434501007068	21.036712145464005
50	19.502482245613383	27.907456493912285	30.47705113402732	22.11301012644701
51	18.838583180201507	28.55718457360433	29.078537461349217	23.525694784844944
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1589.0
1	2856.0
2	4123.0
3	10616.5
4	17110.0
5	11932.5
6	6755.0
7	6852.5
8	6950.0
9	7632.0
10	8314.0
11	8629.5
12	8945.0
13	9173.0
14	9401.0
15	8571.0
16	7741.0
17	7742.5
18	7744.0
19	7956.5
20	8169.0
21	9375.5
22	10582.0
23	12427.5
24	14273.0
25	17769.5
26	26132.0
27	30998.0
28	36789.0
29	42580.0
30	48953.0
31	55326.0
32	64519.0
33	73712.0
34	83100.0
35	92488.0
36	97674.5
37	102861.0
38	109687.0
39	116513.0
40	129200.0
41	141887.0
42	149025.5
43	156164.0
44	163221.5
45	170279.0
46	172732.5
47	175186.0
48	178226.5
49	181267.0
50	172318.5
51	163370.0
52	149170.5
53	134971.0
54	121626.5
55	108282.0
56	98164.5
57	88047.0
58	79426.5
59	70806.0
60	59585.5
61	48365.0
62	38753.0
63	29141.0
64	22879.5
65	16618.0
66	13676.5
67	10735.0
68	7546.0
69	4357.0
70	3207.0
71	2057.0
72	1801.5
73	1546.0
74	1171.5
75	621.5
76	446.0
77	369.5
78	293.0
79	191.5
80	90.0
81	103.5
82	117.0
83	66.5
84	16.0
85	10.0
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2152285.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.187778172292067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.42638628104014	17.179194834859164
2	9.586883332875768	4.254232815069832
3	3.6519962074484345	2.430890452107534
4	1.7845340159365792	1.5837937954604135
5	1.0454370494898806	1.1597962673588498
6	0.6766698306230855	0.900828005864848
7	0.4870006882814572	0.7563824268939771
8	0.3675055827466553	0.6523305877649371
9	0.2842274647037784	0.5675738343588368
>10	2.6746555837118446	13.401645664893941
>50	0.9210809487949192	14.848508538409499
>100	1.0885452523976369	40.78516555138921
>500	0.003596748047778771	0.5552470071075599
>1k	0.001269440487451331	0.42995496602114813
>5k	0.0	0.0
>10k+	2.115734145752218E-4	0.494455252440305
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10533	0.489386860940814	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2799	0.13004783288458546	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08391082036068644	0.0
2	0.0	0.0	0.0	0.29805532259900525	0.0
3	0.0	0.0	0.0	0.44552649858173987	0.0
4	0.0	0.0	0.0	0.6541419932769127	0.0
5	0.0	0.0	0.0	1.1624389892602514	0.0
6	0.0	0.0	0.0	1.6080584123385147	0.0
7	0.0	0.0	0.0	1.9146163263694167	0.0
8	0.0	0.0	0.0	2.5900844915984638	0.0
9	0.0	0.0	0.0	2.809897388124714	0.0
10	0.0	0.0	0.0	3.256631905161259	0.0
11	0.0	0.0	0.0	3.9669467565866046	0.0
12	0.0	0.0	0.0	4.5096258162836245	0.0
13	0.0	0.0	0.0	4.718938244702723	0.0
14	0.0	0.0	0.0	4.81492924961146	0.0
15	0.0	0.0	0.0	4.962679199083764	0.0
16	0.0	0.0	0.0	5.292468237245532	0.0
17	0.0	0.0	0.0	5.695435316419526	0.0
18	0.0	0.0	0.0	6.1207507370074135	0.0
19	0.0	0.0	0.0	6.421733181246907	0.0
20	0.0	0.0	0.0	6.7449245801555096	0.0
21	0.0	0.0	0.0	7.156254864016615	0.0
22	0.0	0.0	0.0	7.618136073986484	0.0
23	0.0	0.0	0.0	8.082526245362487	0.0
24	0.0	0.0	0.0	8.407576134201557	0.0
25	0.0	0.0	0.0	8.711857398067636	0.0
26	0.0	0.0	0.0	8.996392206422476	0.0
27	0.0	0.0	0.0	9.303321818439473	0.0
28	0.0	0.0	0.0	9.61782477692313	0.0
29	0.0	0.0	0.0	9.971123712705333	0.0
30	0.0	0.0	0.0	10.398065311982382	0.0
31	0.0	0.0	0.0	10.777150795549845	0.0
32	0.0	0.0	0.0	11.110703275820814	0.0
33	0.0	0.0	0.0	11.451318017827564	0.0
34	0.0	0.0	0.0	11.802433227941467	0.0
35	0.0	0.0	0.0	12.177662344903208	0.0
36	0.0	0.0	0.0	12.525060575156171	0.0
37	4.6462248261731136E-5	0.0	0.0	12.904564218957992	0.0
38	4.6462248261731136E-5	0.0	0.0	13.345258643720511	0.0
39	4.6462248261731136E-5	0.0	0.0	14.01408270744813	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCAT	20	7.0346624E-4	45.0	15
CCGCTAT	55	1.8189894E-12	45.0	38
CCTCGCG	20	7.0346624E-4	45.0	12
GTTACGC	20	7.0346624E-4	45.0	45
TCGTACT	20	7.0346624E-4	45.0	33
CGCGTCA	20	7.0346624E-4	45.0	39
TCGACGT	55	1.8189894E-12	45.0	26
TACCGCG	40	6.8193913E-9	45.0	1
ACGGTCG	20	7.0346624E-4	45.0	36
CTAGCGT	20	7.0346624E-4	45.0	14
CGTACGA	30	2.166389E-6	44.999996	36
CGTGTCG	30	2.166389E-6	44.999996	37
CTTACCG	30	2.166389E-6	44.999996	32
TACGCGG	110	0.0	42.954544	2
CGTTTTT	5100	0.0	42.2647	1
CGAATAT	220	0.0	40.909092	14
GCCCGTT	55	6.184564E-11	40.909092	3
TACGTAG	110	0.0	40.909092	1
TCGATAG	105	0.0	40.714287	1
TTTACGG	255	0.0	40.588234	2
>>END_MODULE