FastQCFastQC Report
Thu 26 May 2016
SRR1547575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547575_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1660703
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61350.3694218653184826No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT22070.13289552677390237No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT302.1660871E-645.00000415
CGCGAGT600.045.00000433
TCGATAG302.1660871E-645.0000041
TCGCTAA302.1660871E-645.00000423
GTATCCG302.1660871E-645.0000041
GCCCGTA302.1660871E-645.00000421
TGCGTCC302.1660871E-645.00000442
TCGCCGA207.0341956E-445.038
CTATGCG351.2123564E-745.01
TCGCACG207.0341956E-445.01
CGCGGTC253.8915583E-545.021
TCCGATC207.0341956E-445.015
CTATCGT253.8915583E-545.014
GCGAACC207.0341956E-445.042
GCGAAAA253.8915583E-545.043
GCGCGCA207.0341956E-445.012
CTCCCGC351.2123564E-745.030
CCGGACG253.8915583E-545.027
CGCGCGG551.8189894E-1245.02
CGATTCG207.0341956E-445.010