##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547575_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1660703
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.381982208739313	31.0	31.0	33.0	30.0	34.0
2	31.803983614168217	31.0	31.0	34.0	30.0	34.0
3	31.96993622580317	33.0	31.0	34.0	30.0	34.0
4	35.68586255338853	37.0	35.0	37.0	33.0	37.0
5	35.57380097464748	37.0	35.0	37.0	33.0	37.0
6	35.61489260873257	37.0	35.0	37.0	33.0	37.0
7	35.974916646745385	37.0	35.0	37.0	35.0	37.0
8	35.93419955283997	37.0	35.0	37.0	35.0	37.0
9	37.600596253514325	39.0	37.0	39.0	35.0	39.0
10	37.15875746596471	39.0	37.0	39.0	33.0	39.0
11	36.86326031807012	39.0	37.0	39.0	32.0	39.0
12	36.46089216434245	39.0	35.0	39.0	32.0	39.0
13	36.2821588206922	39.0	35.0	39.0	32.0	39.0
14	37.23789804679103	40.0	35.0	41.0	32.0	41.0
15	37.47191279837514	40.0	35.0	41.0	32.0	41.0
16	37.57239193281399	40.0	35.0	41.0	32.0	41.0
17	37.53829432475283	40.0	35.0	41.0	32.0	41.0
18	37.46499584814383	39.0	36.0	41.0	32.0	41.0
19	37.47177008772791	39.0	36.0	41.0	32.0	41.0
20	37.37010169789541	39.0	35.0	41.0	32.0	41.0
21	37.24438264999822	39.0	35.0	41.0	32.0	41.0
22	37.18654870858907	39.0	35.0	41.0	32.0	41.0
23	37.1389056321329	39.0	35.0	41.0	32.0	41.0
24	37.06178829086237	39.0	35.0	41.0	32.0	41.0
25	37.0041711251199	39.0	35.0	41.0	31.0	41.0
26	37.028159159103105	39.0	35.0	41.0	32.0	41.0
27	37.00790448382402	39.0	35.0	41.0	31.0	41.0
28	36.94257431942978	39.0	35.0	41.0	31.0	41.0
29	36.943800306255845	39.0	35.0	41.0	31.0	41.0
30	36.861432176614365	39.0	35.0	41.0	31.0	41.0
31	36.72587873930498	39.0	35.0	41.0	31.0	41.0
32	36.62054081915912	39.0	35.0	41.0	30.0	41.0
33	36.56548822998453	39.0	35.0	41.0	30.0	41.0
34	36.505363692364014	39.0	35.0	41.0	30.0	41.0
35	36.414500365206784	39.0	35.0	41.0	30.0	41.0
36	36.355234500088216	39.0	35.0	41.0	30.0	41.0
37	36.340513023701405	39.0	35.0	41.0	30.0	41.0
38	36.21716285211745	39.0	35.0	41.0	30.0	41.0
39	36.182146958245994	39.0	35.0	41.0	30.0	41.0
40	36.10578953611814	38.0	35.0	41.0	29.0	41.0
41	36.02901662729579	38.0	35.0	41.0	29.0	41.0
42	36.04410421369745	38.0	35.0	41.0	29.0	41.0
43	36.02442820901751	38.0	35.0	41.0	29.0	41.0
44	35.91825028316322	38.0	35.0	40.0	29.0	41.0
45	35.857064749085175	38.0	35.0	40.0	29.0	41.0
46	35.82422745066397	38.0	35.0	40.0	29.0	41.0
47	35.764599690612954	38.0	35.0	40.0	29.0	41.0
48	35.6985270695603	38.0	34.0	40.0	28.0	41.0
49	35.62887102630633	38.0	34.0	40.0	28.0	41.0
50	35.53674678735451	38.0	34.0	40.0	28.0	41.0
51	35.09262643591298	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	7.0
12	6.0
13	3.0
14	11.0
15	33.0
16	99.0
17	269.0
18	524.0
19	1033.0
20	1795.0
21	2850.0
22	4452.0
23	6426.0
24	9302.0
25	13428.0
26	17912.0
27	22131.0
28	25672.0
29	29960.0
30	36533.0
31	45732.0
32	56768.0
33	74078.0
34	121502.0
35	163672.0
36	114712.0
37	154891.0
38	248712.0
39	508043.0
40	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.18233302402657	24.353963351664927	29.658162838267888	12.805540786040611
2	29.52123287547502	25.987428215641206	31.040408790735007	13.45093011814876
3	29.3016270820249	25.11285883147077	32.073164196126584	13.51234989037775
4	27.27194447170867	28.402790866277716	30.275311118243298	14.049953543770318
5	23.731997834651953	31.80761400443065	29.301386220173026	15.159001940744371
6	22.71748771454017	41.159376480924045	25.72537052079752	10.397765283738272
7	89.41942057068603	3.4628106289926617	5.39440225013142	1.7233665501898896
8	89.86772469249469	2.7673822471567764	5.335150234569336	2.029742825779203
9	84.48115045254931	6.140411620861768	6.6115976185988705	2.76684030799005
10	42.10385601760218	38.09844385179048	11.390236544403184	8.407463586204155
11	29.678756526603493	24.619694189749765	28.723317775664885	16.97823150798186
12	30.499071778638324	23.054573876243975	28.416158699056965	18.030195646060733
13	26.580731172280654	27.57862182461283	27.418328262187757	18.422318740918755
14	17.585263590178375	31.087075774536448	29.865725539124092	21.461935096161085
15	16.82137022694606	31.848259441935134	34.980246317372824	16.350124013745987
16	22.5346133535015	28.33763773534461	33.10062064077683	16.027128270377062
17	23.138393800697656	28.035898050403958	27.251109921521188	21.5745982273772
18	23.084862254117684	28.579041526389727	30.770222008390423	17.565874211102166
19	24.640769601789124	30.38917855871881	27.0082609593648	17.96179088012727
20	26.001337987587185	30.94683396128025	27.719104499720903	15.33272355141166
21	26.110809699265914	26.625471261267066	31.05534222555147	16.208376813915553
22	23.890966656891692	22.821058310847874	31.76227176081455	21.525703271445888
23	20.159414416665715	27.112192848450324	32.00283253537809	20.72556019950587
24	20.38431917085716	28.21919391968341	33.400192569050574	17.99629434040885
25	20.789147728401765	30.221719356200357	29.765587224205653	19.22354569119222
26	19.55021457780229	33.86336991021272	26.51268769912501	20.073727812859975
27	18.15213195857417	32.49726170182146	31.488652697080692	17.86195364252368
28	16.57876212664155	30.487269547896283	33.58300671462627	19.350961610835892
29	18.447127511662227	28.743971679463453	32.344133779489766	20.464767029384543
30	20.98201785629339	28.640702160470592	31.644309668857108	18.73297031437891
31	23.65859518529201	28.003863424104132	28.87488009595936	19.462661294644498
32	24.08443893941301	28.987001288008752	28.20721104255246	18.721348730025777
33	23.583747364820802	28.688874530846277	27.895415375295883	19.831962729037038
34	19.951610853957632	27.848387098716625	30.461015606041535	21.738986441284204
35	20.145203567404888	29.196791960994833	29.62088946668971	21.03711500491057
36	22.774391327046438	28.46643861063658	28.661898003435894	20.09727205888109
37	20.548105230134468	30.37358275380968	29.290607652301464	19.787704363754386
38	19.816246493201977	31.58662325533223	27.75192192703933	20.845208324426462
39	19.77843118245707	28.920884709668133	30.208953678050804	21.091730429823997
40	22.375223023020972	27.628841520729473	29.448914104448537	20.547021351801014
41	19.365654183800473	26.710977218683894	30.455716645300214	23.46765195221542
42	20.14670895397913	27.68923763008798	28.61420735676397	23.549846059168917
43	20.49680165568437	27.29259837550724	29.049504938571197	23.161095030237195
44	19.98015298340522	28.01120971058642	30.012289975992097	21.996347330016263
45	19.955223781735807	30.577713173276617	27.785582370839336	21.681480674148236
46	22.08793504919302	29.353412380178757	27.38189790709115	21.176754663537068
47	19.705931765041672	29.141213088673894	29.18221981895619	21.970635327328246
48	20.053194339987343	28.435728724522086	30.666290119304897	20.844786816185675
49	21.195662318909523	27.210283837627802	30.386890371125965	21.207163472336717
50	18.667455890667988	28.434644846188633	30.98320410091389	21.91469516222949
51	18.6567375382594	28.974837764488893	29.17776387469644	23.190660822555266
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	995.0
1	1876.5
2	2758.0
3	6633.0
4	10508.0
5	7566.5
6	4625.0
7	4580.0
8	4535.0
9	4777.5
10	5020.0
11	5078.0
12	5136.0
13	5371.0
14	5606.0
15	5310.5
16	5015.0
17	4962.5
18	4910.0
19	4958.0
20	5006.0
21	6389.0
22	7772.0
23	9135.5
24	10499.0
25	12540.0
26	18429.5
27	22278.0
28	27737.0
29	33196.0
30	37670.5
31	42145.0
32	48970.5
33	55796.0
34	64611.0
35	73426.0
36	75423.5
37	77421.0
38	85837.5
39	94254.0
40	103436.5
41	112619.0
42	121725.0
43	130831.0
44	138803.5
45	146776.0
46	141922.0
47	137068.0
48	137477.5
49	137887.0
50	129247.0
51	120607.0
52	109290.5
53	97974.0
54	88904.5
55	79835.0
56	71322.5
57	62810.0
58	59871.0
59	56932.0
60	48018.5
61	39105.0
62	31317.5
63	23530.0
64	18660.0
65	13790.0
66	10762.5
67	7735.0
68	5607.5
69	3480.0
70	2806.5
71	2133.0
72	1619.0
73	1105.0
74	850.0
75	419.5
76	244.0
77	164.0
78	84.0
79	65.5
80	47.0
81	32.0
82	17.0
83	12.0
84	7.0
85	4.0
86	1.0
87	1.5
88	2.0
89	3.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1660703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.05173858159826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.17021810261858	12.988346467891757
2	9.796136735425598	3.340823254441374
3	3.5557866835294787	1.8189703493841878
4	1.7608932291228663	1.2010516405243812
5	0.9976865272372527	0.8506144924416124
6	0.6493478837736737	0.6643506217573641
7	0.45066167542323265	0.5379195554643436
8	0.3649602508372797	0.4978565431961457
9	0.2860400692205936	0.43897324357906503
>10	2.978645930778098	11.95514141238207
>50	1.316105361319834	16.367111074359478
>100	1.6645911716292643	47.678531581707716
>500	0.00606993777728387	0.6912782195684455
>1k	0.002499386143587476	0.5955075097409693
>5k	3.5705516336963943E-4	0.37352403356107217
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6135	0.3694218653184826	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2207	0.13289552677390237	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.021546296959782E-5	0.0	0.0	0.06557463917389202	0.0
2	6.021546296959782E-5	0.0	0.0	0.2368274158594282	0.0
3	6.021546296959782E-5	0.0	0.0	0.33232914012921033	0.0
4	6.021546296959782E-5	0.0	0.0	0.4773881904229715	0.0
5	6.021546296959782E-5	0.0	0.0	0.820315252034831	0.0
6	6.021546296959782E-5	0.0	0.0	1.066596495580486	0.0
7	6.021546296959782E-5	0.0	0.0	1.2458579288409788	0.0
8	6.021546296959782E-5	0.0	0.0	1.6826608972224413	0.0
9	6.021546296959782E-5	0.0	0.0	1.8043563478839986	0.0
10	6.021546296959782E-5	0.0	0.0	2.06617318087581	0.0
11	6.021546296959782E-5	0.0	0.0	2.512911700647256	0.0
12	6.021546296959782E-5	0.0	0.0	2.8628237559635887	0.0
13	6.021546296959782E-5	0.0	0.0	2.9932504487557376	0.0
14	6.021546296959782E-5	0.0	0.0	3.046179840706014	0.0
15	6.021546296959782E-5	0.0	0.0	3.14565578553179	0.0
16	6.021546296959782E-5	0.0	0.0	3.3460528462946115	0.0
17	6.021546296959782E-5	0.0	0.0	3.609495496786602	0.0
18	6.021546296959782E-5	0.0	0.0	3.8919662335769853	0.0
19	6.021546296959782E-5	0.0	0.0	4.084896576931577	0.0
20	6.021546296959782E-5	0.0	0.0	4.3010098735294635	0.0
21	6.021546296959782E-5	0.0	0.0	4.557889038557767	0.0
22	6.021546296959782E-5	0.0	0.0	4.86444596053599	0.0
23	6.021546296959782E-5	0.0	0.0	5.15643074047557	0.0
24	6.021546296959782E-5	0.0	0.0	5.379890323555747	0.0
25	6.021546296959782E-5	0.0	0.0	5.568846446354345	0.0
26	6.021546296959782E-5	0.0	0.0	5.7527444702634964	0.0
27	6.021546296959782E-5	0.0	0.0	5.9647631153794505	0.0
28	6.021546296959782E-5	0.0	0.0	6.182863522255334	0.0
29	6.021546296959782E-5	0.0	0.0	6.43197489256056	0.0
30	6.021546296959782E-5	0.0	0.0	6.735159748612485	0.0
31	6.021546296959782E-5	0.0	0.0	6.992159344566729	0.0
32	6.021546296959782E-5	0.0	0.0	7.2160404358876935	0.0
33	6.021546296959782E-5	0.0	0.0	7.451783973413669	0.0
34	6.021546296959782E-5	0.0	0.0	7.709566370386517	0.0
35	6.021546296959782E-5	0.0	0.0	8.012510364586564	0.0
36	6.021546296959782E-5	0.0	0.0	8.281854130449574	0.0
37	6.021546296959782E-5	0.0	0.0	8.556617287979849	0.0
38	6.021546296959782E-5	0.0	0.0	8.859501066716927	0.0
39	6.021546296959782E-5	0.0	0.0	9.397586443813253	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCGT	30	2.1660871E-6	45.000004	15
CGCGAGT	60	0.0	45.000004	33
TCGATAG	30	2.1660871E-6	45.000004	1
TCGCTAA	30	2.1660871E-6	45.000004	23
GTATCCG	30	2.1660871E-6	45.000004	1
GCCCGTA	30	2.1660871E-6	45.000004	21
TGCGTCC	30	2.1660871E-6	45.000004	42
TCGCCGA	20	7.0341956E-4	45.0	38
CTATGCG	35	1.2123564E-7	45.0	1
TCGCACG	20	7.0341956E-4	45.0	1
CGCGGTC	25	3.8915583E-5	45.0	21
TCCGATC	20	7.0341956E-4	45.0	15
CTATCGT	25	3.8915583E-5	45.0	14
GCGAACC	20	7.0341956E-4	45.0	42
GCGAAAA	25	3.8915583E-5	45.0	43
GCGCGCA	20	7.0341956E-4	45.0	12
CTCCCGC	35	1.2123564E-7	45.0	30
CCGGACG	25	3.8915583E-5	45.0	27
CGCGCGG	55	1.8189894E-12	45.0	2
CGATTCG	20	7.0341956E-4	45.0	10
>>END_MODULE