FastQCFastQC Report
Thu 26 May 2016
SRR1547574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547574_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2662757
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88620.3328129453795446No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG64150.240915712549061No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34590.1299029539683869No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30920.11612024679683501No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG502.1827873E-1145.00000430
ATCGACC253.8921942E-545.00000426
TCGCACG207.034963E-445.029
ATATGCG351.2127384E-745.01
ACGTCGA351.2127384E-745.020
CGTCGCA207.034963E-445.041
ATTCGCG1200.043.1250041
CGGTCTA6800.042.68382331
TTTACGG1850.042.5675662
GCGTAAG1800.042.51
CGACGGT6850.042.37226528
ACGGGAT11650.041.909875
ACGGTCT7050.041.8085130
CACGACG7050.041.4893626
ACGTACG1150.041.086961
TCACGAC7250.040.96551525
CGGGATT13900.040.9532366
TATCGTC501.0822987E-940.50000437
ATCGTAC451.9299478E-840.027
CGTTAAG451.9299478E-840.034