##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547574_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2662757
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.459195112434216	31.0	31.0	33.0	30.0	34.0
2	31.885591512856788	31.0	31.0	34.0	30.0	34.0
3	32.04192797164743	33.0	31.0	34.0	30.0	34.0
4	35.76073408125488	37.0	35.0	37.0	35.0	37.0
5	35.657137696004554	37.0	35.0	37.0	33.0	37.0
6	35.69053841563462	37.0	35.0	37.0	33.0	37.0
7	36.005032378095336	37.0	35.0	37.0	35.0	37.0
8	35.95687364637479	37.0	35.0	37.0	35.0	37.0
9	37.60533837672758	39.0	37.0	39.0	35.0	39.0
10	37.220891729887484	39.0	37.0	39.0	34.0	39.0
11	36.99641386728117	39.0	37.0	39.0	33.0	39.0
12	36.51074318835703	39.0	35.0	39.0	32.0	39.0
13	36.304320296594845	39.0	35.0	39.0	32.0	39.0
14	37.0969731747959	40.0	35.0	41.0	31.0	41.0
15	37.405203704280936	40.0	35.0	41.0	32.0	41.0
16	37.539678986854604	40.0	35.0	41.0	32.0	41.0
17	37.51234491168364	40.0	35.0	41.0	33.0	41.0
18	37.45656438045229	39.0	35.0	41.0	32.0	41.0
19	37.45608780673565	39.0	35.0	41.0	32.0	41.0
20	37.346424777026215	39.0	35.0	41.0	32.0	41.0
21	37.24189702627765	39.0	35.0	41.0	32.0	41.0
22	37.24221887314539	39.0	35.0	41.0	32.0	41.0
23	37.191844017309876	39.0	35.0	41.0	32.0	41.0
24	37.13434083545739	39.0	35.0	41.0	32.0	41.0
25	37.026375670029225	39.0	35.0	41.0	32.0	41.0
26	37.07626080787695	39.0	35.0	41.0	32.0	41.0
27	37.06981297955465	39.0	35.0	41.0	32.0	41.0
28	37.03651741409374	39.0	35.0	41.0	32.0	41.0
29	36.99480951510033	39.0	35.0	41.0	32.0	41.0
30	36.904486965952955	39.0	35.0	41.0	31.0	41.0
31	36.74027183103828	39.0	35.0	41.0	31.0	41.0
32	36.69096729442454	39.0	35.0	41.0	31.0	41.0
33	36.610070689890215	39.0	35.0	41.0	30.0	41.0
34	36.50600449083412	39.0	35.0	41.0	30.0	41.0
35	36.42391851753652	39.0	35.0	41.0	30.0	41.0
36	36.35151761876882	39.0	35.0	41.0	30.0	41.0
37	36.33627927745566	39.0	35.0	41.0	30.0	41.0
38	36.18226747690458	38.0	35.0	41.0	30.0	41.0
39	36.12896219970504	38.0	35.0	41.0	30.0	41.0
40	36.050184076128616	38.0	35.0	41.0	29.0	41.0
41	35.970225597003406	38.0	35.0	40.0	29.0	41.0
42	35.99035323163173	38.0	35.0	40.0	29.0	41.0
43	35.99356043379099	38.0	35.0	40.0	29.0	41.0
44	35.91308594813571	38.0	35.0	40.0	29.0	41.0
45	35.85497662760815	38.0	35.0	40.0	29.0	41.0
46	35.8527252017364	38.0	35.0	40.0	29.0	41.0
47	35.75665334839041	38.0	34.0	40.0	29.0	41.0
48	35.65759887214642	38.0	34.0	40.0	29.0	41.0
49	35.61351411337948	38.0	34.0	40.0	28.0	41.0
50	35.53617547526868	38.0	34.0	40.0	28.0	41.0
51	35.109221382198974	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	9.0
11	7.0
12	15.0
13	15.0
14	36.0
15	90.0
16	188.0
17	366.0
18	804.0
19	1631.0
20	2743.0
21	4321.0
22	6540.0
23	9704.0
24	13980.0
25	20376.0
26	27590.0
27	33333.0
28	39292.0
29	46315.0
30	56926.0
31	71387.0
32	90951.0
33	123409.0
34	211330.0
35	254852.0
36	189394.0
37	256517.0
38	407568.0
39	792860.0
40	202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.89276603159808	24.333050293361357	29.630754890513856	12.143428784526714
2	31.80008540020738	24.865919045560673	30.311290140256887	13.022705413975064
3	30.29247505499	24.990263850587944	30.35335180791939	14.363909286502674
4	28.898356102340543	26.825542097908293	29.507837177782275	14.768264621968886
5	24.620684501064122	31.290087679799544	28.171477908047937	15.917749911088395
6	23.157614457496496	39.86800147366057	26.225224457207325	10.749159611635609
7	87.52544824781232	3.982901932095193	6.230572297810126	2.2610775222823563
8	87.43103482593418	3.3398466326442855	6.485909153557761	2.7432093878637818
9	81.42305137119159	6.767910102198586	7.959006398255643	3.8500321283541834
10	40.80342291842628	35.75583502362401	13.496462501084402	9.94427955686531
11	33.907825610823664	22.671313980209234	26.510042035379122	16.910818373587976
12	32.28724964388414	20.49676331711831	29.174385796375713	18.041601242621837
13	28.028430682935017	24.567243650096497	29.013988133352008	18.390337533616474
14	18.276658365746478	29.512644225515132	32.49845930364656	19.712238105091828
15	17.515229515873962	29.357767156372137	36.57074227952457	16.556261048229334
16	26.060507962236134	26.707055882305447	32.07844350798815	15.153992647470272
17	25.431573365500494	26.495583337120138	26.480936863559084	21.591906433820284
18	24.910008686485476	28.226984287338276	29.402044572599	17.46096245357725
19	26.41371330541991	28.98214144212183	26.109780201497923	18.49436505096034
20	24.984705701646828	31.13494772523366	26.580157333170096	17.30018923994942
21	26.553267909914425	25.234672183755407	31.710065920397547	16.501993985932625
22	23.794172731495962	21.86602081977439	32.779558930837474	21.56024751789217
23	21.125622803733123	25.48144648572889	33.239983971500216	20.152946739037773
24	21.71016731906066	27.692050006816242	31.468699547123524	19.12908312699957
25	23.258149354221956	27.162824095476985	29.396223538234995	20.182803012066064
26	19.960777494904715	30.9456702207524	26.271041630911117	22.822510653431763
27	18.711696185570066	29.935251320342037	32.61656996864528	18.736482525442614
28	17.400235920889514	29.07640464375833	31.658089716786026	21.865269718566132
29	19.60130045663198	29.00647712126942	31.56859600782197	19.82362641427663
30	22.590157494656854	27.055191292333475	31.353142626232888	19.00150858677679
31	24.8952871028036	25.106722092928496	29.792391870531183	20.205598933736724
32	26.952515757164473	26.51436086732661	27.039042616355907	19.49408075915301
33	25.71372453438297	25.747749419117106	28.205352572540416	20.33317347395951
34	20.746466913804003	26.109855311618745	31.97129892063001	21.172378853947244
35	20.725060529368623	27.962146001306166	30.029138971374408	21.283654497950806
36	25.494515646752596	26.39778995980482	29.37436649307466	18.73332790036793
37	21.98000042812769	29.255429616746852	28.68868620005506	20.075883755070404
38	23.21943008693621	28.7343531535172	26.834405092165753	21.21181166738084
39	21.274904168874592	26.71772151946272	28.657064839187353	23.350309472475335
40	24.599541002051634	25.347412475115078	28.80923794398062	21.243808578852672
41	20.283037468308223	25.196704017677916	29.476028041612505	25.044230472401352
42	20.20556137867631	26.164573034640416	30.302577366241078	23.327288220442195
43	20.46709481939208	25.198994876363106	30.55363294510164	23.780277359143174
44	22.003096790281653	25.930342122844856	29.73767414750952	22.328886939363976
45	20.813427586520287	27.621746933723205	27.561884167424967	24.00294131233154
46	22.42833273933746	26.111019518491545	29.106523802209516	22.354123939961475
47	20.74237341221899	26.521721659167547	30.780240179633367	21.955664748980098
48	20.32784065538087	28.20332459927812	30.89673597703433	20.572098768306684
49	22.704287323251805	25.33111357889586	30.731043050492403	21.233556047359937
50	20.91170917962097	26.380852627558582	29.915009142779457	22.79242905004099
51	19.091640731767864	28.859223729390255	28.92179046003822	23.12734507880366
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1564.0
1	3415.5
2	5267.0
3	10496.0
4	15725.0
5	11318.0
6	6911.0
7	7008.5
8	7106.0
9	7361.0
10	7616.0
11	7667.5
12	7719.0
13	8120.0
14	8521.0
15	7953.5
16	7386.0
17	7420.5
18	7455.0
19	7528.0
20	7601.0
21	10136.0
22	12671.0
23	13643.5
24	14616.0
25	17059.5
26	23559.0
27	27615.0
28	35913.0
29	44211.0
30	50250.5
31	56290.0
32	68190.5
33	80091.0
34	86481.5
35	92872.0
36	103216.5
37	113561.0
38	123152.0
39	132743.0
40	138224.0
41	143705.0
42	161179.0
43	178653.0
44	189228.5
45	199804.0
46	214566.0
47	229328.0
48	233177.5
49	237027.0
50	224628.5
51	212230.0
52	192164.0
53	172098.0
54	160104.5
55	148111.0
56	142063.0
57	136015.0
58	124281.5
59	112548.0
60	96110.0
61	79672.0
62	65736.5
63	51801.0
64	44123.0
65	36445.0
66	28112.0
67	19779.0
68	16433.0
69	13087.0
70	10461.0
71	7835.0
72	5374.5
73	2914.0
74	2725.5
75	1873.0
76	1209.0
77	804.0
78	399.0
79	277.0
80	155.0
81	220.0
82	285.0
83	155.0
84	25.0
85	16.0
86	7.0
87	13.0
88	19.0
89	14.0
90	9.0
91	5.5
92	2.0
93	2.5
94	3.0
95	4.0
96	5.0
97	5.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2662757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.897313787865802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36158194378358	11.821494019224236
2	10.498109568392266	3.3378348397625657
3	4.023466218950073	1.9188691499258188
4	1.9709909333270614	1.2533384536055512
5	1.1729143504764088	0.9323093737907138
6	0.772186403393216	0.7365413734479286
7	0.5435666878916356	0.6048875141430973
8	0.4165057594273498	0.5297058201655941
9	0.32843870611546716	0.46991638540783986
>10	3.033424382782257	10.734956714532764
>50	1.0881824218088887	12.696374270818781
>100	1.7736268832203461	51.882403490923465
>500	0.011017803659832773	1.1356799467673726
>1k	0.0055089018299163866	1.3639887029135929
>5k	4.790349417318597E-4	0.5816999445706816
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8862	0.3328129453795446	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	6415	0.240915712549061	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	3459	0.1299029539683869	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	3092	0.11612024679683501	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7555060412947935E-5	0.0	0.0	0.06669778729339552	0.0
2	3.7555060412947935E-5	0.0	0.0	0.2441454477445745	0.0
3	3.7555060412947935E-5	0.0	0.0	0.35981503381645413	0.0
4	3.7555060412947935E-5	0.0	0.0	0.541619081275535	0.0
5	3.7555060412947935E-5	0.0	0.0	0.9641135109211993	0.0
6	3.7555060412947935E-5	0.0	0.0	1.3009072927045164	0.0
7	3.7555060412947935E-5	0.0	0.0	1.5281905183236772	0.0
8	3.7555060412947935E-5	0.0	0.0	2.0307147817093334	0.0
9	3.7555060412947935E-5	0.0	0.0	2.208650657945881	0.0
10	3.7555060412947935E-5	0.0	0.0	2.5437544620106154	0.0
11	3.7555060412947935E-5	0.0	0.0	3.079327178559666	0.0
12	3.7555060412947935E-5	0.0	0.0	3.500432070970051	0.0
13	3.7555060412947935E-5	0.0	0.0	3.6697678383720334	0.0
14	3.7555060412947935E-5	0.0	0.0	3.7503234429578067	0.0
15	3.7555060412947935E-5	0.0	0.0	3.870424526158414	0.0
16	3.7555060412947935E-5	0.0	0.0	4.116710612346527	0.0
17	3.7555060412947935E-5	0.0	0.0	4.400251318464283	0.0
18	3.7555060412947935E-5	0.0	0.0	4.724689485371741	0.0
19	3.7555060412947935E-5	0.0	0.0	4.949306301701582	0.0
20	3.7555060412947935E-5	0.0	0.0	5.201676307676593	0.0
21	3.7555060412947935E-5	0.0	0.0	5.492164699970744	0.0
22	3.7555060412947935E-5	0.0	0.0	5.826066742102265	0.0
23	3.7555060412947935E-5	0.0	0.0	6.122188393458359	0.0
24	3.7555060412947935E-5	0.0	0.0	6.346354549063245	0.0
25	3.7555060412947935E-5	0.0	0.0	6.546260135641367	0.0
26	3.7555060412947935E-5	0.0	0.0	6.749733452958719	0.0
27	3.7555060412947935E-5	0.0	0.0	7.028692441706096	0.0
28	3.7555060412947935E-5	0.0	0.0	7.258754741795816	0.0
29	3.7555060412947935E-5	0.0	0.0	7.555402163997691	0.0
30	3.7555060412947935E-5	0.0	0.0	7.913226779612259	0.0
31	3.7555060412947935E-5	0.0	0.0	8.204353607933431	0.0
32	3.7555060412947935E-5	0.0	0.0	8.449963703034111	0.0
33	3.7555060412947935E-5	0.0	0.0	8.711647363991531	0.0
34	3.7555060412947935E-5	0.0	0.0	9.017195335511277	0.0
35	3.7555060412947935E-5	0.0	0.0	9.323381743058041	0.0
36	3.7555060412947935E-5	0.0	0.0	9.59355284766879	0.0
37	3.7555060412947935E-5	0.0	0.0	9.90326943089437	0.0
38	3.7555060412947935E-5	0.0	0.0	10.283063756850513	0.0
39	3.7555060412947935E-5	0.0	0.0	11.14600393501923	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	50	2.1827873E-11	45.000004	30
ATCGACC	25	3.8921942E-5	45.000004	26
TCGCACG	20	7.034963E-4	45.0	29
ATATGCG	35	1.2127384E-7	45.0	1
ACGTCGA	35	1.2127384E-7	45.0	20
CGTCGCA	20	7.034963E-4	45.0	41
ATTCGCG	120	0.0	43.125004	1
CGGTCTA	680	0.0	42.683823	31
TTTACGG	185	0.0	42.567566	2
GCGTAAG	180	0.0	42.5	1
CGACGGT	685	0.0	42.372265	28
ACGGGAT	1165	0.0	41.90987	5
ACGGTCT	705	0.0	41.80851	30
CACGACG	705	0.0	41.48936	26
ACGTACG	115	0.0	41.08696	1
TCACGAC	725	0.0	40.965515	25
CGGGATT	1390	0.0	40.953236	6
TATCGTC	50	1.0822987E-9	40.500004	37
ATCGTAC	45	1.9299478E-8	40.0	27
CGTTAAG	45	1.9299478E-8	40.0	34
>>END_MODULE