FastQCFastQC Report
Thu 26 May 2016
SRR1547573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547573_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3229171
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT539321.670150016830945No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTT56610.17530815184454462No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT44340.13731078347972284No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC37160.1150759746077244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCG351.2128476E-745.01
ATTCGAT207.035185E-445.09
CCCACCG351.2128476E-745.044
GCGATCG207.035185E-445.09
CGCGGTA302.1667274E-644.99999638
CGTTTTT193100.044.1261021
CGACGGT5300.042.028328
CGACCGG603.6379788E-1241.2499962
TACTCGC556.184564E-1140.90909245
CGGTCTA5500.040.90909231
CACGACG5550.040.5405426
TACGGGA16350.040.4587144
CTCACGA5750.040.30434824
CGGTTGA451.9301297E-840.037
TGCGGGA23400.039.9038434
GTCTACG850.039.7058831
CTATGCG1250.039.6000021
ACGGGAT19400.039.548975
CGAGGGC4750.039.315794
TCACGAC5750.039.13043625
Produced by FastQC (version 0.11.5)