##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547573_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3229171
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3904110373839	31.0	31.0	33.0	30.0	34.0
2	31.80387319222178	31.0	31.0	34.0	30.0	34.0
3	31.841602689978327	31.0	31.0	34.0	30.0	34.0
4	35.64366365237394	37.0	35.0	37.0	33.0	37.0
5	35.57732712203844	37.0	35.0	37.0	33.0	37.0
6	35.618686963310395	37.0	35.0	37.0	33.0	37.0
7	35.9971961844077	37.0	35.0	37.0	35.0	37.0
8	35.961047587755495	37.0	35.0	37.0	35.0	37.0
9	37.623372376377716	39.0	37.0	39.0	35.0	39.0
10	37.172170504442164	39.0	37.0	39.0	33.0	39.0
11	36.91953352733565	39.0	37.0	39.0	32.0	39.0
12	36.763670613912986	39.0	35.0	39.0	32.0	39.0
13	36.730287123227605	39.0	35.0	39.0	33.0	39.0
14	37.83597338140346	40.0	37.0	41.0	33.0	41.0
15	37.99887091764419	40.0	37.0	41.0	33.0	41.0
16	38.02330474292009	40.0	37.0	41.0	33.0	41.0
17	37.94361617888926	40.0	36.0	41.0	33.0	41.0
18	37.79748765240367	39.0	36.0	41.0	33.0	41.0
19	37.729829110939	39.0	36.0	41.0	33.0	41.0
20	37.57660867138966	39.0	35.0	41.0	33.0	41.0
21	37.45145952320271	39.0	35.0	41.0	32.0	41.0
22	37.395262127648245	39.0	35.0	41.0	32.0	41.0
23	37.30068584166029	39.0	35.0	41.0	32.0	41.0
24	37.22475582742444	39.0	35.0	41.0	32.0	41.0
25	37.16310192306322	39.0	35.0	41.0	32.0	41.0
26	37.183334050751725	39.0	35.0	41.0	32.0	41.0
27	37.13580885001135	39.0	35.0	41.0	32.0	41.0
28	37.050193377805016	39.0	35.0	41.0	32.0	41.0
29	36.994682226490944	39.0	35.0	41.0	31.0	41.0
30	36.8754921928879	39.0	35.0	41.0	31.0	41.0
31	36.70733417338382	39.0	35.0	41.0	31.0	41.0
32	36.52032797272117	39.0	35.0	41.0	30.0	41.0
33	36.33172136130295	39.0	35.0	41.0	30.0	41.0
34	36.174479456182404	39.0	35.0	41.0	29.0	41.0
35	36.03238571137918	39.0	35.0	41.0	29.0	41.0
36	35.9698684275314	39.0	35.0	41.0	28.0	41.0
37	35.92487050081894	39.0	35.0	41.0	28.0	41.0
38	35.79661095680594	39.0	35.0	41.0	27.0	41.0
39	35.73036268441653	39.0	35.0	41.0	27.0	41.0
40	35.67270175534216	39.0	35.0	41.0	27.0	41.0
41	35.6131034869321	39.0	35.0	41.0	26.0	41.0
42	35.5834054622688	38.0	35.0	41.0	26.0	41.0
43	35.562557387019766	38.0	35.0	41.0	26.0	41.0
44	35.453713352436274	38.0	35.0	40.0	26.0	41.0
45	35.40679542830033	38.0	35.0	40.0	26.0	41.0
46	35.36896187907051	38.0	35.0	40.0	26.0	41.0
47	35.31164345276233	38.0	34.0	40.0	26.0	41.0
48	35.21179058030683	38.0	34.0	40.0	25.0	41.0
49	35.1258564504636	38.0	34.0	40.0	25.0	41.0
50	35.044973771906164	38.0	34.0	40.0	24.0	41.0
51	34.628747130455466	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	8.0
11	6.0
12	9.0
13	17.0
14	22.0
15	66.0
16	168.0
17	455.0
18	1052.0
19	2074.0
20	3549.0
21	6056.0
22	9268.0
23	14476.0
24	22228.0
25	35086.0
26	47512.0
27	53641.0
28	56885.0
29	61079.0
30	70306.0
31	84856.0
32	104470.0
33	132880.0
34	198684.0
35	266174.0
36	256158.0
37	317357.0
38	486335.0
39	997999.0
40	293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.46618745182587	23.954878821840033	28.818975520342526	15.75995820599157
2	33.66381030920939	24.644064993770847	28.825943252927765	12.866181444091998
3	27.92326575458531	24.790325442660052	34.60309162939962	12.683317173355018
4	25.394164632346815	26.224873195008875	34.42422219201151	13.9567399806328
5	23.61974636834036	29.94211207768186	32.538134400439	13.900007153538787
6	20.944013184808114	39.11759395832553	29.24849752459687	10.689895332269488
7	84.7091714870473	3.180475732006759	10.022634292206885	2.08771848873906
8	84.74964627144243	2.8611987411010444	9.902758324040443	2.48639666341609
9	79.74213815248558	5.4597913829896285	11.580712201366852	3.2173582631579434
10	35.36511383262144	38.09107662616814	17.01653458426327	9.527274956947155
11	22.37763190614557	25.358923389315706	34.73544758081873	17.527997123719988
12	24.478449732144874	22.603231603405334	34.06796976685347	18.850348897596316
13	23.837604140505412	24.840245375670722	33.2059838268088	18.116166657015068
14	16.307776825693036	28.894598644667624	34.3070713814784	20.490553148160938
15	15.608495183438723	31.159173670270174	36.50599488227784	16.726336264013273
16	21.36845648620033	31.147127234822808	32.850443658759474	14.633972620217387
17	21.577767173060824	30.645450488685793	29.97695074060804	17.79983159764534
18	19.82257985098962	31.141707887256516	32.428818418101734	16.60689384365213
19	21.105014259077638	31.459467460843666	30.39938114147563	17.036137138603067
20	21.958824726222304	32.03540475248911	30.51473582538676	15.491034695901828
21	23.236644946953877	28.79345813523037	32.83406174525908	15.135835172556671
22	21.97183116038141	23.968597513107852	34.49439500106993	19.565176325440802
23	18.39558821753323	27.988948247088803	35.0602058546915	18.55525768068647
24	18.045993847956645	29.259305252029083	35.265831385206916	17.42886951480736
25	19.79669085347292	31.24709097164566	31.018301601246883	17.93791657363453
26	18.881812081181206	33.56929688765321	29.003759788502993	18.545131242662592
27	17.778742593687358	32.288008284479204	32.58848788125498	17.344761240578464
28	16.172200233434523	31.26935674821804	33.95091185942151	18.607531158925926
29	17.7497258584324	29.549100992174154	32.719976737063476	19.981196412329975
30	19.034513811749207	29.779655521494526	32.81473790022269	18.37109276653358
31	19.533124755548712	29.89488633460414	30.871019218245177	19.700969691601962
32	19.53931829562448	30.970487471861972	30.006401023668307	19.483793208845242
33	19.773774755192587	29.14042025027476	30.674591094742272	20.41121389979038
34	17.57314803087232	29.495650741320294	31.114611149425038	21.816590078382347
35	17.572126096759817	30.430937228161653	30.608258280530826	21.388678394547703
36	19.145533017607306	30.26779938256599	30.41864305111126	20.168024548715444
37	18.08061573698017	31.185496215592174	31.128484679194752	19.6054033682329
38	18.270602578804283	30.788707070638253	30.552423516747794	20.388266833809666
39	18.167665942745057	29.656404073986792	30.569084139551606	21.606845843716545
40	19.732835455291774	29.689539513392138	30.912980452258488	19.664644579057597
41	17.716776225229324	29.444863712699014	30.787282556420827	22.05107750565083
42	18.664573663023727	30.057404826192236	30.653161446080123	20.624860064703913
43	19.0073241708166	29.50212299069947	30.78384514167878	20.706707696805154
44	19.084991163366695	29.720693019973236	30.66164659598392	20.53266922067614
45	19.01732673803896	29.939727564752687	29.301328421443152	21.741617275765204
46	19.676226498999277	30.24556457369399	29.18770792875323	20.8905009985535
47	18.717435527570387	29.845554787900674	30.862565036041754	20.57444464848718
48	18.05562480277446	29.332017412518567	31.605882748234766	21.006475036472207
49	19.149868495660343	28.875522541234268	30.671834969408557	21.30277399369683
50	17.717054934532733	28.815569073300857	32.108612396184654	21.358763595981756
51	16.90440054119153	29.48843526713203	31.268799329611223	22.33836486206522
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2291.0
1	3719.0
2	5147.0
3	39322.5
4	73498.0
5	47878.5
6	22259.0
7	21901.0
8	21543.0
9	23972.5
10	26402.0
11	28290.5
12	30179.0
13	30319.0
14	30459.0
15	29358.5
16	28258.0
17	27072.0
18	25886.0
19	24030.5
20	22175.0
21	23910.5
22	25646.0
23	27324.5
24	29003.0
25	32285.0
26	42742.5
27	49918.0
28	56542.5
29	63167.0
30	75821.0
31	88475.0
32	99567.0
33	110659.0
34	119844.5
35	129030.0
36	145855.0
37	162680.0
38	168077.0
39	173474.0
40	186137.5
41	198801.0
42	210636.0
43	222471.0
44	234120.5
45	245770.0
46	263305.5
47	280841.0
48	281941.0
49	283041.0
50	257361.5
51	231682.0
52	204402.5
53	177123.0
54	152729.5
55	128336.0
56	115446.0
57	102556.0
58	89408.5
59	76261.0
60	66148.5
61	56036.0
62	44911.5
63	33787.0
64	25205.5
65	16624.0
66	13630.5
67	10637.0
68	7865.5
69	5094.0
70	3769.5
71	2445.0
72	1691.0
73	937.0
74	647.0
75	297.0
76	237.0
77	257.5
78	278.0
79	156.0
80	34.0
81	45.0
82	56.0
83	46.0
84	36.0
85	21.0
86	6.0
87	4.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3229171.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.438763833448608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.45761813695351	15.218216727022924
2	10.027631422903514	4.0990478092318625
3	4.005321013586231	2.455914308215139
4	2.1851521187787553	1.7864723238351528
5	1.372594216974595	1.4027064519950532
6	0.9402034648566839	1.1529957944157516
7	0.6707154616559441	0.9596016444159807
8	0.4904579383465936	0.8019483177684898
9	0.3888634345270025	0.7153099111584998
>10	3.3829853733691677	15.534993050234194
>50	0.9804834834456239	14.450139100124446
>100	1.0910463581123078	37.80915530014653
>500	0.0030789228844725685	0.40957585474180447
>1k	0.003540761317143454	1.3288482041415555
>5k	1.5394614422362845E-4	0.1781216035717412
>10k+	1.5394614422362845E-4	1.6969535989809479
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53932	1.670150016830945	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTT	5661	0.17530815184454462	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT	4434	0.13731078347972284	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC	3716	0.1150759746077244	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.290310113648364E-5	0.0	0.0	0.09646438668004884	0.0
2	9.290310113648364E-5	0.0	0.0	0.31506538365419484	0.0
3	9.290310113648364E-5	0.0	0.0	0.519235432251807	0.0
4	9.290310113648364E-5	0.0	0.0	0.7649331670574274	0.0
5	9.290310113648364E-5	0.0	0.0	1.4215722858900939	0.0
6	9.290310113648364E-5	0.0	0.0	2.2778601690650633	0.0
7	9.290310113648364E-5	0.0	0.0	2.91467995965528	0.0
8	9.290310113648364E-5	0.0	0.0	3.8708696442523483	0.0
9	9.290310113648364E-5	0.0	0.0	4.288964567066904	0.0
10	9.290310113648364E-5	0.0	0.0	4.791012925608461	0.0
11	9.290310113648364E-5	0.0	0.0	5.519868721724554	0.0
12	9.290310113648364E-5	0.0	0.0	6.055114455072215	0.0
13	9.290310113648364E-5	0.0	0.0	6.291336073561914	0.0
14	9.290310113648364E-5	0.0	0.0	6.407836562387065	0.0
15	9.290310113648364E-5	0.0	0.0	6.550040242526642	0.0
16	9.290310113648364E-5	0.0	0.0	6.817074722893275	0.0
17	9.290310113648364E-5	0.0	0.0	7.157781362461139	0.0
18	9.290310113648364E-5	0.0	0.0	7.508118956846819	0.0
19	9.290310113648364E-5	0.0	0.0	7.7747818248089064	0.0
20	9.290310113648364E-5	0.0	0.0	8.037387924021367	0.0
21	9.290310113648364E-5	0.0	0.0	8.35115266425965	0.0
22	9.290310113648364E-5	0.0	0.0	8.72183603779422	0.0
23	1.2387080151531152E-4	0.0	0.0	9.090971026309848	0.0
24	1.2387080151531152E-4	0.0	0.0	9.359120343890119	0.0
25	1.2387080151531152E-4	0.0	0.0	9.603517435279828	0.0
26	1.2387080151531152E-4	0.0	0.0	9.828157133827846	0.0
27	1.2387080151531152E-4	0.0	0.0	10.055893602413747	0.0
28	1.2387080151531152E-4	0.0	0.0	10.312491967752715	0.0
29	1.2387080151531152E-4	0.0	0.0	10.585936762097765	0.0
30	1.2387080151531152E-4	0.0	0.0	10.912274388689852	0.0
31	1.2387080151531152E-4	0.0	0.0	11.194606913043627	0.0
32	1.2387080151531152E-4	0.0	0.0	11.454425919221991	0.0
33	1.2387080151531152E-4	0.0	0.0	11.713625571392782	0.0
34	1.2387080151531152E-4	0.0	0.0	11.983756821797297	0.0
35	1.2387080151531152E-4	0.0	0.0	12.288633832026857	0.0
36	1.2387080151531152E-4	0.0	0.0	12.570656679376842	0.0
37	1.2387080151531152E-4	0.0	0.0	12.864694994473814	0.0
38	1.2387080151531152E-4	0.0	0.0	13.181959084854906	0.0
39	1.2387080151531152E-4	0.0	0.0	13.639475890251708	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	35	1.2128476E-7	45.0	1
ATTCGAT	20	7.035185E-4	45.0	9
CCCACCG	35	1.2128476E-7	45.0	44
GCGATCG	20	7.035185E-4	45.0	9
CGCGGTA	30	2.1667274E-6	44.999996	38
CGTTTTT	19310	0.0	44.126102	1
CGACGGT	530	0.0	42.0283	28
CGACCGG	60	3.6379788E-12	41.249996	2
TACTCGC	55	6.184564E-11	40.909092	45
CGGTCTA	550	0.0	40.909092	31
CACGACG	555	0.0	40.54054	26
TACGGGA	1635	0.0	40.458714	4
CTCACGA	575	0.0	40.304348	24
CGGTTGA	45	1.9301297E-8	40.0	37
TGCGGGA	2340	0.0	39.903843	4
GTCTACG	85	0.0	39.705883	1
CTATGCG	125	0.0	39.600002	1
ACGGGAT	1940	0.0	39.54897	5
CGAGGGC	475	0.0	39.31579	4
TCACGAC	575	0.0	39.130436	25
>>END_MODULE