FastQCFastQC Report
Thu 26 May 2016
SRR1547572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547572_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1958454
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72620.37080268415801443No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC21330.10891243807615597No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGT351.2125201E-745.00000441
CCGTCGA253.8918144E-545.00000410
CGTACGT351.2125201E-745.00000425
CGTCGCG302.1662872E-645.00000445
GCTACGC253.8918144E-545.00000410
CGGTATG253.8918144E-545.00000438
CGCGTGA253.8918144E-545.00000423
ATCGCAC600.045.00000419
CACGGTA207.034506E-445.011
CCGTACA207.034506E-445.025
CGACTCG207.034506E-445.011
TCGACGC207.034506E-445.025
TATCCGG207.034506E-445.02
GTCGGTA700.041.78571742
CGTTTTT34800.041.3146551
TACGGGT1200.041.2500044
CGGGATA5300.041.1792456
TATAGCG1750.041.142861
CCGAACC2850.041.0526318
TGCACGG1050.040.7142872
Produced by FastQC (version 0.11.5)