##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547572_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1958454
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.417692731103205	31.0	31.0	33.0	30.0	34.0
2	31.84698900254997	31.0	31.0	34.0	30.0	34.0
3	32.02294309695301	33.0	31.0	34.0	30.0	34.0
4	35.728386267944	37.0	35.0	37.0	35.0	37.0
5	35.625732337854245	37.0	35.0	37.0	33.0	37.0
6	35.65344143901261	37.0	35.0	37.0	33.0	37.0
7	36.001175416936015	37.0	35.0	37.0	35.0	37.0
8	35.95043896869674	37.0	35.0	37.0	35.0	37.0
9	37.60185687281907	39.0	37.0	39.0	35.0	39.0
10	37.13826314021161	39.0	37.0	39.0	33.0	39.0
11	36.91378199334781	39.0	37.0	39.0	33.0	39.0
12	36.64903081716497	39.0	35.0	39.0	32.0	39.0
13	36.53734169911573	39.0	35.0	39.0	32.0	39.0
14	37.56867661941511	40.0	36.0	41.0	32.0	41.0
15	37.76925574968828	40.0	36.0	41.0	33.0	41.0
16	37.83568161417118	40.0	36.0	41.0	33.0	41.0
17	37.77030453612901	40.0	36.0	41.0	33.0	41.0
18	37.68814993867612	40.0	36.0	41.0	33.0	41.0
19	37.70196083237084	40.0	36.0	41.0	33.0	41.0
20	37.60342647823232	40.0	36.0	41.0	32.0	41.0
21	37.49197377114806	39.0	35.0	41.0	32.0	41.0
22	37.45051760214945	39.0	35.0	41.0	32.0	41.0
23	37.35962039445399	39.0	35.0	41.0	32.0	41.0
24	37.31040095912388	39.0	35.0	41.0	32.0	41.0
25	37.2479205536612	39.0	35.0	41.0	32.0	41.0
26	37.27671571555931	39.0	35.0	41.0	32.0	41.0
27	37.249292554229	39.0	35.0	41.0	32.0	41.0
28	37.202074697695224	39.0	35.0	41.0	32.0	41.0
29	37.185967094453076	39.0	35.0	41.0	32.0	41.0
30	37.10343413733486	39.0	35.0	41.0	31.0	41.0
31	37.0034787643723	39.0	35.0	41.0	31.0	41.0
32	36.92539421400758	39.0	35.0	41.0	31.0	41.0
33	36.8626641218022	39.0	35.0	41.0	31.0	41.0
34	36.804966059963625	39.0	35.0	41.0	31.0	41.0
35	36.71787287319488	39.0	35.0	41.0	30.0	41.0
36	36.688786665400364	39.0	35.0	41.0	30.0	41.0
37	36.65696258375229	39.0	35.0	41.0	30.0	41.0
38	36.53431073693842	39.0	35.0	41.0	30.0	41.0
39	36.47550261583882	39.0	35.0	41.0	30.0	41.0
40	36.41391985719348	39.0	35.0	41.0	30.0	41.0
41	36.35452453823271	39.0	35.0	41.0	30.0	41.0
42	36.33808504054729	39.0	35.0	41.0	30.0	41.0
43	36.31792883570408	39.0	35.0	41.0	30.0	41.0
44	36.23274378668072	39.0	35.0	41.0	30.0	41.0
45	36.15806140966293	39.0	35.0	40.0	30.0	41.0
46	36.156123656721064	38.0	35.0	40.0	30.0	41.0
47	36.1088317621961	38.0	35.0	40.0	29.0	41.0
48	36.021111039626156	38.0	35.0	40.0	29.0	41.0
49	35.949761904032464	38.0	35.0	40.0	29.0	41.0
50	35.85816210133095	38.0	35.0	40.0	29.0	41.0
51	35.410515130812364	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	6.0
12	7.0
13	8.0
14	19.0
15	45.0
16	102.0
17	237.0
18	520.0
19	983.0
20	1737.0
21	2809.0
22	4417.0
23	6589.0
24	9630.0
25	14026.0
26	19257.0
27	23315.0
28	27411.0
29	32824.0
30	40326.0
31	50343.0
32	63421.0
33	81800.0
34	130417.0
35	169098.0
36	141579.0
37	189485.0
38	308267.0
39	639555.0
40	214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.56489353336867	24.917102980207858	29.787219919385393	11.730783567038081
2	29.96761731447356	25.774258675465443	30.45167259481203	13.806451415248965
3	29.31970830052684	25.387473997346888	31.434948178512233	13.857869523614033
4	27.068136397382837	27.920390267016735	30.368596862627356	14.642876472973072
5	25.227500875690723	31.73671681847008	28.49482295729182	14.540959348547375
6	23.41326372740948	40.92335076545071	25.156475464830937	10.506910042308883
7	89.14163927260994	3.4164703383383017	5.545854025675355	1.8960363633764183
8	89.18590888527379	3.2027813775559704	5.357746467366606	2.2535632698036308
9	83.82954105636384	6.211532157507912	6.950073884809141	3.0088529013191017
10	37.11790013959991	41.32376864608512	12.689141537151244	8.869189677163721
11	27.64726667054728	24.45607606816397	30.01127419893447	17.885383062354286
12	28.08097611687586	22.010167203314452	31.12899256250083	18.779864117308858
13	26.919396626114274	24.845209537727207	28.962998365036913	19.27239547112161
14	17.9645271219033	29.721198455516447	31.306632680675676	21.007641741904585
15	16.942802843467348	31.46624837754678	34.38896190566641	17.201986873319466
16	23.868163357423764	30.744097129674735	30.495329479272936	14.892410033628567
17	24.29906446615545	29.392010228476135	27.296837199137684	19.01208810623073
18	22.587561413237175	30.57636278411441	29.421880728370436	17.414195074277977
19	23.870767452286344	30.613994507912874	27.486578699320997	18.028659340479784
20	24.59608446254035	32.51038829607435	27.67677974565652	15.216747495728775
21	25.671933065571107	27.322112237509792	31.26864353209215	15.73731116482695
22	24.45735258525347	23.072382603829347	32.242319707279314	20.22794510363787
23	20.378829423616793	26.941046355952196	32.875472183671405	19.804652036759606
24	19.796124902601747	28.65556198920169	32.55537275830834	18.99294034988823
25	20.77628578460357	30.919950123924277	28.69860614545963	19.60515794601252
26	19.6146552331584	34.007640720690915	26.718779200328424	19.658924845822266
27	18.47380637993029	32.38891492983751	30.80077448844854	18.33650420178365
28	17.203722936561185	30.7906644730997	33.136698640866726	18.86891394947239
29	18.93779481162182	29.303317821097664	31.7614301893228	19.997457177957713
30	20.42738813370138	29.056541537355486	31.21926785107028	19.296802477872852
31	22.487839898205422	28.96468336759505	29.114801777320277	19.432674956879254
32	23.207591293949207	29.454151080393004	28.8935558353681	18.444701790289688
33	21.917594183983898	28.569218373267898	29.775373840794835	19.737813601953377
34	19.586061250353595	28.907444341301865	30.561606246559787	20.944888161784757
35	19.307116735956015	29.69051098468486	29.99084992550246	21.011522353856666
36	21.9344442095653	28.770448527256704	29.5537704740576	19.741336789120396
37	19.88522579544886	30.544756220978385	29.308117525354184	20.261900458218573
38	20.294272931608297	30.499567516010078	28.981329150442136	20.22483040193949
39	20.42473297815522	29.84200803286674	28.667969735311626	21.06528925366641
40	21.131361778218942	28.41373859176677	30.198666907673093	20.256232722341196
41	20.342984823743627	27.281570054747267	30.113497687461642	22.261947434047467
42	20.173871839726644	27.912220557643934	29.522163910921574	22.39174369170785
43	20.088038830628648	28.47005852575552	29.308372828772082	22.13352981484375
44	20.736662694145483	28.739914238475862	29.189248253979926	21.334174813398732
45	19.392337016851048	30.08638446448066	29.03029634599536	21.490982172672936
46	20.622848430445647	29.281514909209	29.255116535798138	20.84052012454722
47	19.61981236219998	29.074668080026388	29.625051188335288	21.680468369438344
48	19.20969295168536	29.711701168370563	30.439520152119986	20.63908572782409
49	21.032712537542366	28.55660638442363	30.061671093627933	20.349009984406067
50	19.28761155482845	29.50669252379683	30.390501895883183	20.815194025491536
51	18.698013841530106	29.333035138941227	29.657678965142914	22.311272054385757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1436.0
1	2405.5
2	3375.0
3	7811.5
4	12248.0
5	8843.5
6	5439.0
7	5484.5
8	5530.0
9	5907.0
10	6284.0
11	6298.0
12	6312.0
13	6376.5
14	6441.0
15	6242.0
16	6043.0
17	5801.5
18	5560.0
19	7388.0
20	9216.0
21	9413.5
22	9611.0
23	11449.0
24	13287.0
25	17764.0
26	26621.5
27	31002.0
28	36999.0
29	42996.0
30	51240.5
31	59485.0
32	67120.5
33	74756.0
34	82005.5
35	89255.0
36	94749.0
37	100243.0
38	106214.5
39	112186.0
40	122324.5
41	132463.0
42	139509.5
43	146556.0
44	149642.0
45	152728.0
46	159819.0
47	166910.0
48	165030.5
49	163151.0
50	151197.0
51	139243.0
52	123161.5
53	107080.0
54	99125.0
55	91170.0
56	82590.5
57	74011.0
58	66798.0
59	59585.0
60	50129.0
61	40673.0
62	34022.5
63	27372.0
64	21644.0
65	15916.0
66	12592.5
67	9269.0
68	7044.0
69	4819.0
70	3568.0
71	2317.0
72	1748.0
73	1179.0
74	849.5
75	376.0
76	232.0
77	241.5
78	251.0
79	141.5
80	32.0
81	23.5
82	15.0
83	10.5
84	6.0
85	4.5
86	3.0
87	3.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1958454.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.475395017330424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.90514361144265	13.26472368457155
2	9.672212447238957	3.380514664140819
3	3.4433952088108644	1.8052407442425853
4	1.6785299453687632	1.173318953749488
5	0.9681879271931296	0.8459733239355144
6	0.6407310588598346	0.6718217012068799
7	0.4519581750824061	0.5528703348613782
8	0.3723878107776108	0.5206099274382116
9	0.2921438399960828	0.459479610523019
>10	3.4572106731929604	14.556151083419122
>50	1.551800986818902	19.8138551318466
>100	1.5600840199942088	41.65289423039597
>500	0.004142863482467655	0.5191466338917536
>1k	0.0017755129210575665	0.4078602949999264
>5k	2.9591882017626105E-4	0.37553968077716054
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7262	0.37080268415801443	No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC	2133	0.10891243807615597	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.1060683580007495E-5	0.0	0.0	0.04758855709656699	0.0
2	5.1060683580007495E-5	0.0	0.0	0.1819802762791467	0.0
3	5.1060683580007495E-5	0.0	0.0	0.2691408631502195	0.0
4	5.1060683580007495E-5	0.0	0.0	0.39214604989445756	0.0
5	5.1060683580007495E-5	0.0	0.0	0.7115306256874044	0.0
6	5.1060683580007495E-5	0.0	0.0	0.967651014524722	0.0
7	5.1060683580007495E-5	0.0	0.0	1.1410530959624274	0.0
8	5.1060683580007495E-5	0.0	0.0	1.539071124468586	0.0
9	5.1060683580007495E-5	0.0	0.0	1.655642665081743	0.0
10	5.1060683580007495E-5	0.0	0.0	1.9205965521784019	0.0
11	5.1060683580007495E-5	0.0	0.0	2.365998895046807	0.0
12	5.1060683580007495E-5	0.0	0.0	2.7105053271611177	0.0
13	5.1060683580007495E-5	0.0	0.0	2.8419865873796373	0.0
14	5.1060683580007495E-5	0.0	0.0	2.8996851598250455	0.0
15	5.1060683580007495E-5	0.0	0.0	2.9991003107553205	0.0
16	5.1060683580007495E-5	0.0	0.0	3.206253504039411	0.0
17	5.1060683580007495E-5	0.0	0.0	3.469777691995829	0.0
18	5.1060683580007495E-5	0.0	0.0	3.7437182594025695	0.0
19	5.1060683580007495E-5	0.0	0.0	3.941680529642259	0.0
20	5.1060683580007495E-5	0.0	0.0	4.165581627140591	0.0
21	5.1060683580007495E-5	0.0	0.0	4.42507202109419	0.0
22	5.1060683580007495E-5	0.0	0.0	4.722908988416373	0.0
23	5.1060683580007495E-5	0.0	0.0	5.018754589078937	0.0
24	5.1060683580007495E-5	0.0	0.0	5.224171719121307	0.0
25	5.1060683580007495E-5	0.0	0.0	5.419836258599895	0.0
26	5.1060683580007495E-5	0.0	0.0	5.604267447690883	0.0
27	5.1060683580007495E-5	0.0	0.0	5.814075796521133	0.0
28	5.1060683580007495E-5	0.0	0.0	6.021790657324604	0.0
29	5.1060683580007495E-5	0.0	0.0	6.258099500932879	0.0
30	5.1060683580007495E-5	0.0	0.0	6.54005659566168	0.0
31	5.1060683580007495E-5	0.0	0.0	6.783922420439796	0.0
32	5.1060683580007495E-5	0.0	0.0	6.993883951320787	0.0
33	5.1060683580007495E-5	0.0	0.0	7.223554906063661	0.0
34	5.1060683580007495E-5	0.0	0.0	7.480236962420358	0.0
35	5.1060683580007495E-5	0.0	0.0	7.7500926751406976	0.0
36	5.1060683580007495E-5	0.0	0.0	7.991507587106973	0.0
37	5.1060683580007495E-5	0.0	0.0	8.246708883639851	0.0
38	5.1060683580007495E-5	0.0	0.0	8.528972342470132	0.0
39	5.1060683580007495E-5	0.0	0.0	9.002253818573221	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGT	35	1.2125201E-7	45.000004	41
CCGTCGA	25	3.8918144E-5	45.000004	10
CGTACGT	35	1.2125201E-7	45.000004	25
CGTCGCG	30	2.1662872E-6	45.000004	45
GCTACGC	25	3.8918144E-5	45.000004	10
CGGTATG	25	3.8918144E-5	45.000004	38
CGCGTGA	25	3.8918144E-5	45.000004	23
ATCGCAC	60	0.0	45.000004	19
CACGGTA	20	7.034506E-4	45.0	11
CCGTACA	20	7.034506E-4	45.0	25
CGACTCG	20	7.034506E-4	45.0	11
TCGACGC	20	7.034506E-4	45.0	25
TATCCGG	20	7.034506E-4	45.0	2
GTCGGTA	70	0.0	41.785717	42
CGTTTTT	3480	0.0	41.314655	1
TACGGGT	120	0.0	41.250004	4
CGGGATA	530	0.0	41.179245	6
TATAGCG	175	0.0	41.14286	1
CCGAACC	285	0.0	41.05263	18
TGCACGG	105	0.0	40.714287	2
>>END_MODULE