FastQCFastQC Report
Thu 26 May 2016
SRR1547571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547571_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2537182
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129480.510329964503926No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA207.0348993E-445.039
CGCGAAG551.8189894E-1245.02
TACGACG207.0348993E-445.01
GTCCGCG207.0348993E-445.09
ACGTACT406.8212103E-945.010
CGACTTA207.0348993E-445.015
CGCGTCA207.0348993E-445.033
CCGCCGA253.8921426E-545.038
AATCGCG453.8562575E-1045.01
TACCGAT253.8921426E-545.023
ACGCGTC207.0348993E-445.032
GCGAACG302.1665437E-644.9999961
CGTCGGG1150.043.043483
CGTTTTT70950.042.1458741
TTTCGCG750.042.01
CTAAGCG700.041.7857131
GCTATCG650.041.538469
CGTAAGG3050.041.3114782
TCGACGT603.6379788E-1241.24999626
CTCGCGC1050.040.71428733