##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547571_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2537182
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.525853486269412	31.0	31.0	33.0	30.0	34.0
2	31.955357558109746	31.0	31.0	34.0	30.0	34.0
3	32.097151091250055	33.0	31.0	34.0	30.0	34.0
4	35.80465650473636	37.0	35.0	37.0	35.0	37.0
5	35.74006161166207	37.0	35.0	37.0	33.0	37.0
6	35.77691509714321	37.0	35.0	37.0	35.0	37.0
7	36.08637259762997	37.0	35.0	37.0	35.0	37.0
8	36.05388970913399	37.0	35.0	37.0	35.0	37.0
9	37.737272690725376	39.0	38.0	39.0	35.0	39.0
10	37.31664224324467	39.0	37.0	39.0	34.0	39.0
11	37.06683517382671	39.0	37.0	39.0	33.0	39.0
12	36.85123534693215	39.0	35.0	39.0	33.0	39.0
13	36.77837813763459	39.0	35.0	39.0	33.0	39.0
14	37.88921094347981	40.0	37.0	41.0	33.0	41.0
15	38.07240276811045	40.0	37.0	41.0	33.0	41.0
16	38.12034887524821	40.0	37.0	41.0	33.0	41.0
17	38.057887451511164	40.0	37.0	41.0	33.0	41.0
18	37.96169569230745	40.0	36.0	41.0	33.0	41.0
19	37.955723712370656	40.0	36.0	41.0	33.0	41.0
20	37.86113530680889	40.0	36.0	41.0	33.0	41.0
21	37.747322817204285	40.0	36.0	41.0	33.0	41.0
22	37.69607462137127	40.0	36.0	41.0	33.0	41.0
23	37.59405434848584	39.0	35.0	41.0	33.0	41.0
24	37.55030699413759	39.0	35.0	41.0	33.0	41.0
25	37.51174649670382	39.0	35.0	41.0	33.0	41.0
26	37.527398901616046	39.0	35.0	41.0	33.0	41.0
27	37.50830764210057	39.0	35.0	41.0	33.0	41.0
28	37.449801787967914	39.0	35.0	41.0	33.0	41.0
29	37.43348880766141	39.0	35.0	41.0	32.0	41.0
30	37.36333656789304	39.0	35.0	41.0	32.0	41.0
31	37.2338566961298	39.0	35.0	41.0	32.0	41.0
32	37.12808580543296	39.0	35.0	41.0	31.0	41.0
33	37.03588902963997	40.0	35.0	41.0	31.0	41.0
34	36.95367695340736	40.0	35.0	41.0	31.0	41.0
35	36.85494339783271	40.0	35.0	41.0	31.0	41.0
36	36.81288295439586	39.0	35.0	41.0	31.0	41.0
37	36.79660111099637	39.0	35.0	41.0	31.0	41.0
38	36.666104757167595	39.0	35.0	41.0	30.0	41.0
39	36.61464451505647	39.0	35.0	41.0	30.0	41.0
40	36.5593315733755	39.0	35.0	41.0	30.0	41.0
41	36.51635830618379	39.0	35.0	41.0	30.0	41.0
42	36.50853545390122	39.0	35.0	41.0	30.0	41.0
43	36.4954445522631	39.0	35.0	41.0	30.0	41.0
44	36.38896736615663	39.0	35.0	41.0	30.0	41.0
45	36.32870838591792	39.0	35.0	41.0	30.0	41.0
46	36.295437221295124	39.0	35.0	41.0	30.0	41.0
47	36.25977797414612	39.0	35.0	41.0	30.0	41.0
48	36.1578932847545	39.0	35.0	40.0	30.0	41.0
49	36.10283889764313	39.0	35.0	40.0	29.0	41.0
50	36.01694123637957	38.0	35.0	40.0	29.0	41.0
51	35.597641793139005	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	11.0
12	5.0
13	5.0
14	20.0
15	41.0
16	88.0
17	219.0
18	526.0
19	1076.0
20	1887.0
21	3109.0
22	4843.0
23	7767.0
24	11808.0
25	18156.0
26	25012.0
27	29514.0
28	33045.0
29	37878.0
30	45990.0
31	57743.0
32	73821.0
33	97423.0
34	155653.0
35	210324.0
36	180549.0
37	246761.0
38	410575.0
39	883060.0
40	266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.77801907785882	24.612030197281868	29.702480941453945	12.907469783405368
2	30.728619389543198	25.032496683328198	30.963919813399276	13.274964113729325
3	29.74705795642567	24.455557386107895	32.842381823613756	12.955002833852674
4	26.461483646029333	28.010012683362877	31.302208513224517	14.22629515738327
5	24.254428732349513	32.06498390734287	29.483891971486475	14.19669538882114
6	22.45136533366546	40.80736029185135	26.45226081534553	10.289013559137658
7	89.0549830481219	3.408860696631144	5.830287302999943	1.7058689522470205
8	89.85118135001746	2.7708299995822134	5.712597677265565	1.6653909731347611
9	84.52952921784878	5.940921857399272	7.035522087102936	2.4940268376490136
10	38.120324044550216	40.4094385030321	13.134611549348845	8.335625903068838
11	25.725115502159486	25.722790087585363	30.91201183044811	17.640082579807046
12	26.38569089643549	23.447431047516496	31.59446188724341	18.572416168804605
13	25.038881719955448	25.43211326582011	30.739576427706012	18.78942858651843
14	18.03075222825954	30.2739023057865	31.442482249992317	20.252863215961643
15	17.380857975501954	31.752511250671024	34.136534154822165	16.730096619004865
16	22.762222024277328	30.58712382477883	31.665722049108027	14.984932101835815
17	22.872265371581545	29.36919779503402	28.435918274684273	19.322618558700164
18	21.945213232633687	29.711073151236295	31.02110924640014	17.322604369729884
19	23.479159161621045	29.938017848148064	28.94061206488143	17.642210925349463
20	24.51318037097851	31.102656411719774	29.088689735304758	15.295473481996954
21	25.16441469315169	26.622134320675457	32.704669984258125	15.508781001914723
22	24.460050560030773	22.785121445761476	32.957036586259875	19.797791407947873
23	20.15673294229582	27.00779841572264	33.516003187788655	19.31946545419288
24	19.15373039852876	28.799155913923403	33.458616685756084	18.58849700179175
25	20.29846498989824	30.958401880511527	29.786353521347703	18.95677960824253
26	20.534593103687477	33.533148193547014	27.336036594930913	18.5962221078346
27	18.947596191365065	31.95111742082358	31.21612876017566	17.8851576276357
28	17.13842365269815	30.44645594994762	33.53882378166013	18.876296615694105
29	18.627122532005984	29.502692357111158	32.85046165391367	19.01972345696919
30	20.3816675350842	28.330880480785375	32.51619316233522	18.771258821795204
31	21.75965303238002	28.749336862708315	30.536674152662286	18.954335952249384
32	22.165496996273816	28.715795713512076	30.90491734530672	18.213789944907383
33	22.3133381838591	28.86245448690713	29.42504715861929	19.399160170614486
34	19.694211924883593	29.056764552168506	31.286285335462733	19.962738187485172
35	19.843629664722513	29.3085005332688	31.058473534811455	19.78939626719723
36	22.016197497853916	29.087862045371597	30.471483716974184	18.4244567398003
37	20.5331742066592	29.631772572878102	30.62653763111988	19.208515589342824
38	20.363497770360976	30.583103616532043	29.25272999729621	19.80066861581077
39	20.461086354861415	29.547269372082884	29.567409827123164	20.424234445932534
40	21.54279826989156	29.092118736456428	29.575489657423077	19.789593336228933
41	19.338896460719017	29.25336061819767	29.809410598057212	21.5983323230261
42	20.106204442566593	28.736409134228445	30.315405043863624	20.84198137934133
43	20.445320832325	28.454442763664566	30.29230855334777	20.807927850662665
44	20.281990018847683	29.412947120072587	30.412954214557725	19.892108646522008
45	19.958875634463745	30.1800974466948	29.01589243499284	20.845134483848614
46	20.64952376297798	30.053145576470275	29.56212837707346	19.735202283478284
47	20.636517206885436	29.502101150016042	29.92560249915063	19.93577914394789
48	19.546843702974403	29.76057689200065	30.570175888052177	20.122403516972767
49	20.992896843821214	28.221152443931892	30.24166181219952	20.544288900047373
50	20.022134793641133	29.739254022770144	30.083888345416295	20.15472283817243
51	19.528634524444836	29.85867785598353	29.915551978533667	20.69713564103797
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1446.0
1	2659.0
2	3872.0
3	12644.0
4	21416.0
5	15213.5
6	9011.0
7	8901.5
8	8792.0
9	9301.5
10	9811.0
11	10072.0
12	10333.0
13	10243.0
14	10153.0
15	9697.5
16	9242.0
17	9428.5
18	9615.0
19	10048.0
20	10481.0
21	13166.0
22	15851.0
23	17818.0
24	19785.0
25	25563.5
26	36708.5
27	42075.0
28	48602.0
29	55129.0
30	65345.0
31	75561.0
32	87271.0
33	98981.0
34	112041.5
35	125102.0
36	127762.0
37	130422.0
38	143724.5
39	157027.0
40	166437.5
41	175848.0
42	187871.0
43	199894.0
44	200033.5
45	200173.0
46	205337.5
47	210502.0
48	206410.0
49	202318.0
50	186761.0
51	171204.0
52	158092.0
53	144980.0
54	126247.5
55	107515.0
56	98023.0
57	88531.0
58	78888.5
59	69246.0
60	57657.0
61	46068.0
62	37781.0
63	29494.0
64	23296.0
65	17098.0
66	12918.0
67	8738.0
68	6937.5
69	5137.0
70	3959.0
71	2781.0
72	1934.0
73	1087.0
74	813.5
75	364.0
76	188.0
77	205.5
78	223.0
79	135.5
80	48.0
81	61.5
82	75.0
83	51.0
84	27.0
85	20.5
86	14.0
87	9.0
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2537182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.987969322815434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.21995018156898	12.948221754710145
2	9.195792651727242	3.1243568693302795
3	3.307173400115055	1.685464807991573
4	1.6453762747669933	1.1180640672092008
5	0.9822300578292025	0.8343047045174862
6	0.6465494463588181	0.6590137296256143
7	0.503231482311351	0.5984216688646118
8	0.3929980700463481	0.5340991326298427
9	0.3088199449179955	0.47216013754864145
>10	3.6604074379199174	14.962234325785545
>50	1.5075110843677015	18.607388174707488
>100	1.6231380834884308	43.00700665913215
>500	0.005175222786332435	0.609541259116045
>1k	0.0014114243962724822	0.3222939916587493
>5k	0.0	0.0
>10k+	2.3523739937874704E-4	0.5174287171726579
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12948	0.510329964503926	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.882761268210164E-5	0.0	0.0	0.049227844119972476	0.0
2	7.882761268210164E-5	0.0	0.0	0.16439498624852297	0.0
3	7.882761268210164E-5	0.0	0.0	0.23900532165213217	0.0
4	7.882761268210164E-5	0.0	0.0	0.335805630025753	0.0
5	7.882761268210164E-5	0.0	0.0	0.5844279204251015	0.0
6	7.882761268210164E-5	0.0	0.0	0.802701579941841	0.0
7	7.882761268210164E-5	0.0	0.0	0.9536170444217246	0.0
8	7.882761268210164E-5	0.0	0.0	1.274208945199832	0.0
9	7.882761268210164E-5	0.0	0.0	1.3772760487816798	0.0
10	7.882761268210164E-5	0.0	0.0	1.5856568429068154	0.0
11	7.882761268210164E-5	0.0	0.0	1.9270198196266566	0.0
12	7.882761268210164E-5	0.0	0.0	2.2007092908589136	0.0
13	7.882761268210164E-5	0.0	0.0	2.3035399116027153	0.0
14	7.882761268210164E-5	0.0	0.0	2.3471709952222586	0.0
15	7.882761268210164E-5	0.0	0.0	2.4185099846995604	0.0
16	7.882761268210164E-5	0.0	0.0	2.5754557615496245	0.0
17	7.882761268210164E-5	0.0	0.0	2.7923105240380863	0.0
18	7.882761268210164E-5	0.0	0.0	3.016141530248914	0.0
19	7.882761268210164E-5	0.0	0.0	3.189049898667104	0.0
20	7.882761268210164E-5	0.0	0.0	3.3645595783038034	0.0
21	7.882761268210164E-5	0.0	0.0	3.58236027214445	0.0
22	7.882761268210164E-5	0.0	0.0	3.8376040820090953	0.0
23	7.882761268210164E-5	0.0	0.0	4.087999993693791	0.0
24	7.882761268210164E-5	0.0	0.0	4.277777471225951	0.0
25	7.882761268210164E-5	0.0	0.0	4.446232079527602	0.0
26	7.882761268210164E-5	0.0	0.0	4.611415341902946	0.0
27	7.882761268210164E-5	0.0	0.0	4.7966208178995435	0.0
28	7.882761268210164E-5	0.0	0.0	4.9906944003228775	0.0
29	7.882761268210164E-5	0.0	0.0	5.2129488542800635	0.0
30	7.882761268210164E-5	0.0	0.0	5.470675733944195	0.0
31	7.882761268210164E-5	0.0	0.0	5.693324325964791	0.0
32	7.882761268210164E-5	0.0	0.0	5.896936049522659	0.0
33	7.882761268210164E-5	0.0	0.0	6.113278432528688	0.0
34	7.882761268210164E-5	0.0	0.0	6.338134197704382	0.0
35	7.882761268210164E-5	0.0	0.0	6.581002072377937	0.0
36	7.882761268210164E-5	0.0	0.0	6.820598601125186	0.0
37	7.882761268210164E-5	0.0	0.0	7.070915685197199	0.0
38	7.882761268210164E-5	0.0	0.0	7.34326508701386	0.0
39	7.882761268210164E-5	0.0	0.0	7.747296015815972	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGTA	20	7.0348993E-4	45.0	39
CGCGAAG	55	1.8189894E-12	45.0	2
TACGACG	20	7.0348993E-4	45.0	1
GTCCGCG	20	7.0348993E-4	45.0	9
ACGTACT	40	6.8212103E-9	45.0	10
CGACTTA	20	7.0348993E-4	45.0	15
CGCGTCA	20	7.0348993E-4	45.0	33
CCGCCGA	25	3.8921426E-5	45.0	38
AATCGCG	45	3.8562575E-10	45.0	1
TACCGAT	25	3.8921426E-5	45.0	23
ACGCGTC	20	7.0348993E-4	45.0	32
GCGAACG	30	2.1665437E-6	44.999996	1
CGTCGGG	115	0.0	43.04348	3
CGTTTTT	7095	0.0	42.145874	1
TTTCGCG	75	0.0	42.0	1
CTAAGCG	70	0.0	41.785713	1
GCTATCG	65	0.0	41.53846	9
CGTAAGG	305	0.0	41.311478	2
TCGACGT	60	3.6379788E-12	41.249996	26
CTCGCGC	105	0.0	40.714287	33
>>END_MODULE