FastQCFastQC Report
Thu 26 May 2016
SRR1547570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547570_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2277239
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78550.3449352483424006No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG253.8920152E-545.00000416
ACCGGAT253.8920152E-545.00000411
ACGTTGC302.1664437E-645.00000445
CGTGACG253.8920152E-545.00000424
GCTATCG253.8920152E-545.00000431
CCAATCG502.1827873E-1145.00000424
TATGCGT253.8920152E-545.00000426
CTAACGA253.8920152E-545.00000416
TCGGACA253.8920152E-545.00000412
TATTCGC600.045.00000415
TCTAACG600.045.0000041
GTATCGC253.8920152E-545.00000413
ACGTCGT253.8920152E-545.00000420
AACCTCG302.1664437E-645.00000425
CGTAAAC253.8920152E-545.00000421
CACGTTA253.8920152E-545.00000431
ATACGCG502.1827873E-1145.0000041
CCAGTCG502.1827873E-1145.00000428
AGGTGCG502.1827873E-1145.0000041
CGATAAG253.8920152E-545.00000412