##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547570_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2277239
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.505077420507906	31.0	31.0	33.0	30.0	34.0
2	31.942964704187833	31.0	31.0	34.0	30.0	34.0
3	32.10095953916124	33.0	31.0	34.0	30.0	34.0
4	35.79466055165927	37.0	35.0	37.0	35.0	37.0
5	35.715051428506186	37.0	35.0	37.0	33.0	37.0
6	35.74726807331159	37.0	35.0	37.0	33.0	37.0
7	36.04250410255577	37.0	35.0	37.0	35.0	37.0
8	35.98544772858712	37.0	35.0	37.0	35.0	37.0
9	37.67953297831277	39.0	38.0	39.0	35.0	39.0
10	37.2569220885467	39.0	37.0	39.0	34.0	39.0
11	37.01667896957675	39.0	37.0	39.0	33.0	39.0
12	36.425221507272624	38.0	35.0	39.0	33.0	39.0
13	36.140441561030705	38.0	35.0	39.0	32.0	39.0
14	37.07601090618947	39.0	35.0	41.0	31.0	41.0
15	37.363455043585674	39.0	35.0	41.0	32.0	41.0
16	37.50097903645599	39.0	35.0	41.0	33.0	41.0
17	37.45676365107044	39.0	35.0	41.0	33.0	41.0
18	37.391387992213375	39.0	35.0	41.0	32.0	41.0
19	37.3856090643099	39.0	35.0	41.0	32.0	41.0
20	37.27603821996725	39.0	35.0	41.0	32.0	41.0
21	37.14580068231749	39.0	35.0	41.0	32.0	41.0
22	37.103875350808586	39.0	35.0	41.0	32.0	41.0
23	37.06739784449502	39.0	35.0	41.0	32.0	41.0
24	37.02745517708067	39.0	35.0	41.0	32.0	41.0
25	36.95579603194921	38.0	35.0	41.0	32.0	41.0
26	36.99024432657266	39.0	35.0	41.0	32.0	41.0
27	36.97075098397665	39.0	35.0	41.0	32.0	41.0
28	36.94458816136558	39.0	35.0	41.0	32.0	41.0
29	36.9370795072454	39.0	35.0	41.0	32.0	41.0
30	36.85185744667117	38.0	35.0	41.0	32.0	41.0
31	36.700374444667425	38.0	35.0	41.0	31.0	41.0
32	36.617800327501854	38.0	35.0	41.0	31.0	41.0
33	36.53566972987903	38.0	35.0	41.0	31.0	41.0
34	36.43719258277238	38.0	35.0	41.0	30.0	41.0
35	36.36522516960231	38.0	35.0	41.0	30.0	41.0
36	36.311660304430056	38.0	35.0	41.0	30.0	41.0
37	36.27651906541211	38.0	35.0	41.0	30.0	41.0
38	36.15060562373998	38.0	35.0	41.0	30.0	41.0
39	36.121864679113614	38.0	35.0	41.0	30.0	41.0
40	36.033063723219215	38.0	35.0	41.0	30.0	41.0
41	35.99202718730884	38.0	35.0	41.0	30.0	41.0
42	36.00611486102249	38.0	35.0	40.0	30.0	41.0
43	35.971497062890634	38.0	35.0	40.0	30.0	41.0
44	35.87181582609467	38.0	35.0	40.0	30.0	41.0
45	35.78456674947162	38.0	35.0	40.0	29.0	41.0
46	35.80158779996302	38.0	35.0	40.0	29.0	41.0
47	35.74734448162885	38.0	35.0	40.0	29.0	41.0
48	35.67527870372851	38.0	35.0	40.0	29.0	41.0
49	35.625781922758215	38.0	35.0	40.0	29.0	41.0
50	35.528612499610276	37.0	34.0	40.0	29.0	41.0
51	35.11275145033086	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	5.0
11	9.0
12	12.0
13	28.0
14	30.0
15	42.0
16	115.0
17	311.0
18	606.0
19	1173.0
20	2134.0
21	3358.0
22	5435.0
23	8152.0
24	12167.0
25	17722.0
26	23942.0
27	28586.0
28	32872.0
29	38472.0
30	47447.0
31	59088.0
32	77010.0
33	103448.0
34	187944.0
35	267552.0
36	150271.0
37	201666.0
38	327914.0
39	679510.0
40	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.82049007592088	23.63871337176291	31.035609349743265	12.50518720257294
2	31.23449053876207	25.759175914341885	29.69661945891494	13.309714087981103
3	30.485118162827877	24.361562400784457	31.547588988244097	13.605730448143563
4	27.033438299625118	27.54756966660065	30.74126167696935	14.677730356804886
5	24.549553208951718	31.239452688101686	28.932887588874074	15.278106514072523
6	22.696914992234017	40.80621313792711	25.931577669274063	10.565294200564807
7	88.26987417657962	3.7687743798520925	5.8313598177442065	2.129991625824079
8	88.49765000511584	2.9903317130964293	6.026640155029841	2.4853781267578854
9	84.23819370737986	5.621544335047837	7.112692168015741	3.027569789556564
10	43.23239677521771	31.38757065024795	14.115426619691654	11.264605954842684
11	36.28424596627758	24.329769514750097	23.782747441089846	15.60323707788247
12	36.487781914853905	21.27646680914915	25.981243075496245	16.254508200500695
13	26.56401018953215	28.33352142660476	25.996085610689086	19.106382773174005
14	16.844960059089097	32.709610190234756	30.19204396200838	20.253385788667767
15	17.439451897670818	27.761995996028528	38.721363897245745	16.077188209054913
16	23.47526983333765	26.880226449661194	34.87143861491921	14.77306510208195
17	22.847579898289112	26.876230382493887	26.32617832383865	23.95001139537835
18	23.37927639567037	28.733479445943093	30.959288858130396	16.92795530025614
19	27.12135177730576	29.466340599295904	25.67222851883355	17.74007910456478
20	29.13484267571388	29.38896619985869	25.493239839999227	15.982951284428204
21	25.29896949771192	28.383406396957017	30.09209836999981	16.22552573533125
22	23.564764172754813	23.531829553244084	31.29447545909762	21.608930814903488
23	20.837645938788153	27.34895195453793	30.922709474060472	20.89069263261344
24	21.803508546972893	26.750244484658836	34.12426188028574	17.321985088082542
25	21.638176757029015	27.543485773781317	30.288564353587834	20.52977311560183
26	19.0437630832776	32.7956793292228	27.707061050684622	20.453496536814978
27	18.085409568341312	32.587752098045044	31.405838385869906	17.920999947743738
28	16.790420329179327	29.952850798708436	33.929245019956184	19.327483852156053
29	19.00037721117546	28.692552692097756	32.88811582798292	19.41895426874386
30	20.72654648897195	28.71714387466577	31.964848661032065	18.59146097533021
31	24.808682795262158	27.39225000098804	28.313409352290208	19.485657851459596
32	26.16352521628165	27.421671594417624	28.865832703550222	17.548970485750505
33	25.40980547057204	27.794579312931138	27.66727603031566	19.12833918618116
34	19.70983282826265	28.718022131186054	30.452886148533377	21.11925889201792
35	19.82233748851131	28.912819427385532	30.679915459027356	20.584927625075807
36	24.940333447653057	28.019237330820353	28.38806115651453	18.65236806501206
37	21.119698020278065	31.4721028403255	28.911414216953073	18.496784922443364
38	21.635849377250256	30.160163250321993	27.43045415962049	20.773533212807262
39	20.45384783942309	28.944129272333736	29.385277522473487	21.216745365769686
40	23.684997490381992	27.207377003467798	27.619411049960064	21.48821445619015
41	18.329784445110946	26.72639982013306	29.327488243438655	25.616327491317335
42	20.812966930568113	27.338808091728623	28.180309576640834	23.667915401062427
43	21.26136079700023	26.58877702340422	28.651098984340244	23.498763195255307
44	21.1633912821623	27.96004284135306	29.188065020843222	21.688500855641415
45	19.975549338475233	31.244107447659204	26.325783108404522	22.454560105461045
46	21.995407596655422	30.23586896237066	26.89340029746548	20.875323143508435
47	19.678215593532343	28.42319141732598	29.234744354896435	22.66384863424524
48	21.41852480130544	27.350093688014304	30.798787479048094	20.432594031632163
49	21.830427109319665	26.81896805737123	29.80420588265	21.54639895065911
50	20.225018103062524	29.268952446361578	29.207123187333433	21.29890626324246
51	18.416775753445293	30.013274847304128	28.850726691401295	22.719222707849283
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1674.0
1	2867.0
2	4060.0
3	9060.5
4	14061.0
5	10186.0
6	6311.0
7	6348.5
8	6386.0
9	6888.5
10	7391.0
11	7437.5
12	7484.0
13	7385.5
14	7287.0
15	7342.5
16	7398.0
17	7233.0
18	7068.0
19	6748.0
20	6428.0
21	7329.5
22	8231.0
23	10841.5
24	13452.0
25	14977.0
26	23799.0
27	31096.0
28	33130.0
29	35164.0
30	40025.5
31	44887.0
32	54712.5
33	64538.0
34	71596.0
35	78654.0
36	91561.0
37	104468.0
38	116243.5
39	128019.0
40	140171.0
41	152323.0
42	168716.0
43	185109.0
44	189639.5
45	194170.0
46	195131.0
47	196092.0
48	194607.0
49	193122.0
50	182228.0
51	171334.0
52	149713.0
53	128092.0
54	119856.0
55	111620.0
56	106765.5
57	101911.0
58	93704.5
59	85498.0
60	75880.5
61	66263.0
62	55249.0
63	44235.0
64	34555.0
65	24875.0
66	18511.5
67	12148.0
68	8528.5
69	4909.0
70	3842.0
71	2775.0
72	1990.5
73	1206.0
74	855.0
75	365.0
76	226.0
77	145.5
78	65.0
79	112.0
80	159.0
81	87.0
82	15.0
83	14.0
84	13.0
85	9.0
86	5.0
87	5.0
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2277239.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.968552664041765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.93807240675841	10.467764109555798
2	10.47614472993457	2.9267315875242925
3	3.9892803961526826	1.6717341991586445
4	2.088798466369766	1.16709965528223
5	1.2137933689182983	0.8477468298499963
6	0.8211434751207345	0.6882111526174949
7	0.550787632201369	0.538559423297538
8	0.3947905466769157	0.4411722034017868
9	0.29470915913578755	0.37049943689673415
>10	2.5227542908437357	7.61123238334349
>50	0.7696602728571494	7.967640412804042
>100	1.909826338012021	62.125384291660346
>500	0.026737568732159975	2.2692334445465687
>1k	0.003183043896685711	0.5577378045024621
>5k	3.1830438966857117E-4	0.349253065558581
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7855	0.3449352483424006	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.391282601430943E-5	0.0	0.0	0.047689329051540046	0.0
2	4.391282601430943E-5	0.0	0.0	0.20208682531785202	0.0
3	4.391282601430943E-5	0.0	0.0	0.29715809363883194	0.0
4	4.391282601430943E-5	0.0	0.0	0.46292901184285007	0.0
5	4.391282601430943E-5	0.0	0.0	0.810894245180238	0.0
6	4.391282601430943E-5	0.0	0.0	1.087632874722416	0.0
7	4.391282601430943E-5	0.0	0.0	1.27241804659063	0.0
8	4.391282601430943E-5	0.0	0.0	1.6742643174475758	0.0
9	4.391282601430943E-5	0.0	0.0	1.7883059266067374	0.0
10	4.391282601430943E-5	0.0	0.0	2.061268053111685	0.0
11	4.391282601430943E-5	0.0	0.0	2.544484790573146	0.0
12	4.391282601430943E-5	0.0	0.0	2.893460018908863	0.0
13	4.391282601430943E-5	0.0	0.0	3.02304676847709	0.0
14	4.391282601430943E-5	0.0	0.0	3.082109519466336	0.0
15	4.391282601430943E-5	0.0	0.0	3.182582065387076	0.0
16	4.391282601430943E-5	0.0	0.0	3.4002140311139937	0.0
17	4.391282601430943E-5	0.0	0.0	3.6422615281048674	0.0
18	4.391282601430943E-5	0.0	0.0	3.9182975524308166	0.0
19	4.391282601430943E-5	0.0	0.0	4.099877087999986	0.0
20	4.391282601430943E-5	0.0	0.0	4.305389113746954	0.0
21	4.391282601430943E-5	0.0	0.0	4.5718521420017835	0.0
22	4.391282601430943E-5	0.0	0.0	4.845472960896946	0.0
23	4.391282601430943E-5	0.0	0.0	5.105612542205715	0.0
24	4.391282601430943E-5	0.0	0.0	5.308182408609724	0.0
25	4.830410861574038E-4	0.0	0.0	5.488883687658608	0.0
26	4.830410861574038E-4	0.0	0.0	5.6599680578103575	0.0
27	4.830410861574038E-4	0.0	0.0	5.861220539433937	0.0
28	4.830410861574038E-4	0.0	0.0	6.04956265020931	0.0
29	4.830410861574038E-4	0.0	0.0	6.276723699181333	0.0
30	4.830410861574038E-4	0.0	0.0	6.539805439833061	0.0
31	4.830410861574038E-4	0.0	0.0	6.770479514886229	0.0
32	4.830410861574038E-4	0.0	0.0	6.980558474538685	0.0
33	4.830410861574038E-4	0.0	0.0	7.191120475277298	0.0
34	4.830410861574038E-4	0.0	0.0	7.425395402063639	0.0
35	4.830410861574038E-4	0.0	0.0	7.701431426389589	0.0
36	4.830410861574038E-4	0.0	0.0	7.943435010554448	0.0
37	4.830410861574038E-4	0.0	0.0	8.21002977728732	0.0
38	4.830410861574038E-4	0.0	0.0	8.51399435895837	0.0
39	4.830410861574038E-4	0.0	0.0	9.027510946369704	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	25	3.8920152E-5	45.000004	16
ACCGGAT	25	3.8920152E-5	45.000004	11
ACGTTGC	30	2.1664437E-6	45.000004	45
CGTGACG	25	3.8920152E-5	45.000004	24
GCTATCG	25	3.8920152E-5	45.000004	31
CCAATCG	50	2.1827873E-11	45.000004	24
TATGCGT	25	3.8920152E-5	45.000004	26
CTAACGA	25	3.8920152E-5	45.000004	16
TCGGACA	25	3.8920152E-5	45.000004	12
TATTCGC	60	0.0	45.000004	15
TCTAACG	60	0.0	45.000004	1
GTATCGC	25	3.8920152E-5	45.000004	13
ACGTCGT	25	3.8920152E-5	45.000004	20
AACCTCG	30	2.1664437E-6	45.000004	25
CGTAAAC	25	3.8920152E-5	45.000004	21
CACGTTA	25	3.8920152E-5	45.000004	31
ATACGCG	50	2.1827873E-11	45.000004	1
CCAGTCG	50	2.1827873E-11	45.000004	28
AGGTGCG	50	2.1827873E-11	45.000004	1
CGATAAG	25	3.8920152E-5	45.000004	12
>>END_MODULE