FastQCFastQC Report
Thu 26 May 2016
SRR1547569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547569_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1158476
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51770.44688021158832814No Hit
GGGAATGGTTACATCAGTGCGGCAGAACTGCGCCACGTCATGACAAACTTA13510.1166187301247501No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAC302.165516E-645.00000438
GTCATCG302.165516E-645.0000041
CGGCGTA302.165516E-645.00000435
TTTCGCG302.165516E-645.0000041
ACTAGGT302.165516E-645.00000443
CGGGTTA207.0333114E-445.06
GTTTCGC207.0333114E-445.017
CGCATCG351.211938E-745.021
GGCGTAG1000.045.01
CTAAGCG453.8562575E-1045.01
TCGGATA253.890825E-545.039
CCATACG551.8189894E-1245.02
GTCGTAC207.0333114E-445.030
TATAGCG800.045.01
TTACGAT207.0333114E-445.010
CCGCGTT207.0333114E-445.045
CACGTTA207.0333114E-445.040
CGTCCTA207.0333114E-445.040
CGATAAT253.890825E-545.043
GTTGCGC253.890825E-545.035