##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547569_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1158476
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.543427744726692	31.0	31.0	33.0	30.0	34.0
2	31.97821448178469	31.0	31.0	34.0	30.0	34.0
3	32.135969152576315	33.0	31.0	34.0	30.0	34.0
4	35.842144334453195	37.0	35.0	37.0	35.0	37.0
5	35.762491411129794	37.0	35.0	37.0	35.0	37.0
6	35.803116335599526	37.0	35.0	37.0	35.0	37.0
7	36.08527410149196	37.0	35.0	37.0	35.0	37.0
8	36.038104371605456	37.0	35.0	37.0	35.0	37.0
9	37.70008960047511	39.0	38.0	39.0	35.0	39.0
10	37.330049133516795	39.0	37.0	39.0	34.0	39.0
11	37.13665798859881	39.0	37.0	39.0	33.0	39.0
12	36.81748607653503	39.0	35.0	39.0	33.0	39.0
13	36.708929662763836	39.0	35.0	39.0	33.0	39.0
14	37.74582468691626	40.0	36.0	41.0	33.0	41.0
15	37.962547346686506	40.0	37.0	41.0	33.0	41.0
16	38.025738988118874	40.0	36.0	41.0	33.0	41.0
17	37.98523922808932	40.0	36.0	41.0	33.0	41.0
18	37.88131994102597	40.0	36.0	41.0	33.0	41.0
19	37.895567107130404	40.0	36.0	41.0	33.0	41.0
20	37.80654497805738	40.0	36.0	41.0	33.0	41.0
21	37.660636042524835	40.0	35.0	41.0	33.0	41.0
22	37.61760278158546	39.0	35.0	41.0	33.0	41.0
23	37.53380648369064	39.0	35.0	41.0	33.0	41.0
24	37.48623622759556	39.0	35.0	41.0	32.0	41.0
25	37.43272454500568	39.0	35.0	41.0	32.0	41.0
26	37.47193554290292	39.0	35.0	41.0	33.0	41.0
27	37.447670042366006	39.0	35.0	41.0	33.0	41.0
28	37.418140729717315	39.0	35.0	41.0	33.0	41.0
29	37.371675373507955	39.0	35.0	41.0	32.0	41.0
30	37.271586981517096	39.0	35.0	41.0	32.0	41.0
31	37.14389767245933	39.0	35.0	41.0	32.0	41.0
32	37.061593852613264	39.0	35.0	41.0	31.0	41.0
33	36.97876779493058	39.0	35.0	41.0	31.0	41.0
34	36.889086178738275	39.0	35.0	41.0	31.0	41.0
35	36.80509911297256	39.0	35.0	41.0	31.0	41.0
36	36.75357711337999	39.0	35.0	41.0	31.0	41.0
37	36.718285057264886	39.0	35.0	41.0	31.0	41.0
38	36.607359151160665	39.0	35.0	41.0	30.0	41.0
39	36.53167782500457	39.0	35.0	41.0	30.0	41.0
40	36.462848604545975	39.0	35.0	41.0	30.0	41.0
41	36.38451551866417	39.0	35.0	41.0	30.0	41.0
42	36.38258367027025	39.0	35.0	41.0	30.0	41.0
43	36.36417413912761	39.0	35.0	41.0	30.0	41.0
44	36.2648867995539	39.0	35.0	41.0	30.0	41.0
45	36.207220520753125	39.0	35.0	41.0	30.0	41.0
46	36.19190125647834	39.0	35.0	41.0	30.0	41.0
47	36.12607770899009	39.0	35.0	40.0	30.0	41.0
48	36.04898072985543	38.0	35.0	40.0	29.0	41.0
49	35.98071345457308	38.0	35.0	40.0	29.0	41.0
50	35.86496828592047	38.0	35.0	40.0	29.0	41.0
51	35.427963980263726	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	5.0
12	5.0
13	13.0
14	14.0
15	20.0
16	61.0
17	144.0
18	326.0
19	622.0
20	1055.0
21	1718.0
22	2521.0
23	3940.0
24	5590.0
25	8170.0
26	11201.0
27	13141.0
28	15039.0
29	17488.0
30	21303.0
31	26805.0
32	34511.0
33	45925.0
34	75117.0
35	98731.0
36	83170.0
37	111925.0
38	185718.0
39	394067.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.17789924003605	24.04624696584133	29.840324702453913	12.93552909166871
2	31.594266950718016	25.148816203356823	30.04619862647133	13.210718219453835
3	29.13845431411613	25.766783256623356	31.45054364527189	13.64421878398862
4	26.919504590513743	27.831651238351075	30.77275662163049	14.476087549504694
5	24.169943960858923	31.323134877200737	29.489777949651092	15.01714321228925
6	23.66307113828858	40.18581308546746	25.74692958680197	10.404186189441992
7	88.09979662936479	4.006643210562843	5.944534025737261	1.9490261343351092
8	88.1441652654004	3.4182840214212464	6.191582734558161	2.245967978620187
9	82.36053228551994	7.1752025937524815	7.534467697215998	2.929797423511579
10	35.56008065769166	42.20777987632027	13.091423559918375	9.1407159060697
11	29.64066584029363	23.78866717998474	30.199417165310287	16.37124981441135
12	28.398430351599863	23.002116573843566	30.985104568415743	17.614348506140825
13	26.451130623336176	25.141996899374696	30.231010396417364	18.175862080871767
14	18.417299969960535	30.48401520618468	31.46832562780757	19.63035919604722
15	17.96075188437223	31.750161418967675	33.79836958210615	16.49071711455395
16	23.549042017270967	30.053449531971317	31.189856328486737	15.207652122270984
17	23.8464154630739	29.56306388738308	27.541615018351695	19.048905631191325
18	23.01808583000425	30.2468070119709	29.83462756241821	16.90047959560664
19	23.475151837413982	30.81600309371968	28.26161267043944	17.447232398426898
20	25.612442553837973	31.67074673968213	27.17967398547747	15.53713672100242
21	26.54176694208598	27.11484743749547	30.648196423577183	15.695189196841367
22	24.234166266715924	23.507004029431773	32.94975467769725	19.30907502615505
23	20.59507490875944	27.199786616209572	32.86231221017958	19.34282626485141
24	20.251174819331606	28.16225800102894	33.18204261460747	18.40452456503199
25	21.24144134190091	30.309043950845766	29.333624520490716	19.115890186762606
26	20.088978968921236	33.387312296499886	27.01497484626354	19.508733888315337
27	18.252687151050175	32.50382398944821	31.125806663236872	18.11768219626475
28	17.00984742023141	31.370870005075634	32.65436659887646	18.964915975816503
29	19.68664003397567	28.83909550133106	32.60550930705513	18.86875515763814
30	21.063362555633436	29.095207842026937	31.605574910485846	18.23585469185378
31	22.81834064754039	28.889247597705953	29.79612870702544	18.496283047728223
32	23.237857322896634	29.45870264036545	29.20224501845528	18.101195018282638
33	23.138675294093275	28.51133730867105	28.831930916134645	19.51805648110103
34	20.170896937010347	29.183945114098176	30.207790234756697	20.437367714134776
35	20.56469016190236	29.48882842631181	29.784475466043318	20.162005945742507
36	22.761282926879797	29.262755551258724	29.337854215365706	18.638107306495773
37	20.822615228973238	31.12338969473688	28.674051080902842	19.37994399538704
38	21.22409096088309	30.928996371094435	27.41765906242339	20.42925360559908
39	19.437692278476206	30.212537851453114	29.45585407034759	20.893915799723082
40	22.370856193827063	28.854719476277452	28.70003349227779	20.0743908376177
41	20.118155231528316	28.32341800779645	29.38282709352632	22.17559966714891
42	20.15941633663537	28.744488448616977	29.633242294186502	21.46285292056115
43	20.428390402563366	28.214740745600253	29.43669096295478	21.9201778888816
44	20.63115679565222	28.667490737831425	29.889527275489524	20.81182519102683
45	20.05091171504632	29.651801159454315	28.76287467327765	21.534412452221712
46	21.25939596504373	29.246182052973047	29.412780238865544	20.081641743117682
47	20.385748172599175	29.51576036102604	29.62055320956153	20.47793825681326
48	20.222689119153095	30.272703103042275	29.476570943204695	20.02803683459994
49	21.560222222989513	28.028547850797082	30.746687889951975	19.664542036261434
50	19.759580690493372	29.020022857616386	30.42531739975623	20.79507905213401
51	19.243730556351622	30.303864732631492	29.211999212758833	21.240405498258056
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	842.0
1	1534.0
2	2226.0
3	5686.5
4	9147.0
5	6447.0
6	3747.0
7	3817.5
8	3888.0
9	4036.5
10	4185.0
11	4391.5
12	4598.0
13	4593.0
14	4588.0
15	4516.5
16	4445.0
17	4317.5
18	4190.0
19	4350.0
20	4510.0
21	5739.5
22	6969.0
23	7938.5
24	8908.0
25	11107.0
26	16412.0
27	19518.0
28	22046.0
29	24574.0
30	28162.0
31	31750.0
32	36217.0
33	40684.0
34	46943.0
35	53202.0
36	56900.0
37	60598.0
38	64040.0
39	67482.0
40	71603.5
41	75725.0
42	80179.5
43	84634.0
44	88941.0
45	93248.0
46	93823.0
47	94398.0
48	95096.0
49	95794.0
50	89092.0
51	82390.0
52	74107.0
53	65824.0
54	59815.0
55	53806.0
56	48274.5
57	42743.0
58	38708.5
59	34674.0
60	29628.5
61	24583.0
62	20064.5
63	15546.0
64	12914.0
65	10282.0
66	7913.5
67	5545.0
68	4312.5
69	3080.0
70	2239.5
71	1399.0
72	1109.5
73	820.0
74	572.5
75	227.0
76	129.0
77	114.0
78	99.0
79	74.5
80	50.0
81	27.0
82	4.0
83	5.0
84	6.0
85	5.0
86	4.0
87	3.5
88	3.0
89	2.5
90	2.0
91	2.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1158476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.351733249071437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.90648230223486	14.689219973882107
2	8.575153412899821	3.3188816283260376
3	2.955323220367099	1.715718798759926
4	1.4833194468650996	1.1481920903557439
5	0.9422171640626417	0.9116767610818408
6	0.6354214402562589	0.7377903727547943
7	0.5164776770720979	0.6996316765059508
8	0.4241086653900275	0.6565790208998006
9	0.36265212210972714	0.6316152416349409
>10	5.162243881258629	25.036810249644358
>50	2.3580432800424274	33.02691145780659
>100	0.6731469630896272	16.193855654791374
>500	0.003606949567794385	0.4726381651068952
>1k	0.0013526060879228943	0.30878096110638775
>5k	4.5086869597429815E-4	0.45169794734325214
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5177	0.44688021158832814	No Hit
GGGAATGGTTACATCAGTGCGGCAGAACTGCGCCACGTCATGACAAACTTA	1351	0.1166187301247501	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.059388368857015594	0.0
2	0.0	0.0	0.0	0.212261626481688	0.0
3	0.0	0.0	0.0	0.3007399376422127	0.0
4	0.0	0.0	0.0	0.4194303550526727	0.0
5	0.0	0.0	0.0	0.731737213373432	0.0
6	0.0	0.0	0.0	0.981202890694326	0.0
7	0.0	0.0	0.0	1.1528076541939583	0.0
8	0.0	0.0	0.0	1.53676036447885	0.0
9	0.0	0.0	0.0	1.6565729458357359	0.0
10	0.0	0.0	0.0	1.9407393851922699	0.0
11	0.0	0.0	0.0	2.4724724551911303	0.0
12	0.0	0.0	0.0	2.8144734979403974	0.0
13	0.0	0.0	0.0	2.9636349825115067	0.0
14	0.0	0.0	0.0	3.020520062564956	0.0
15	0.0	0.0	0.0	3.11866624772546	0.0
16	0.0	0.0	0.0	3.326698179332157	0.0
17	0.0	0.0	0.0	3.5773723409030485	0.0
18	0.0	0.0	0.0	3.8365058922239217	0.0
19	0.0	0.0	0.0	4.016052123652108	0.0
20	0.0	0.0	0.0	4.225378859812374	0.0
21	0.0	0.0	0.0	4.460083765222586	0.0
22	0.0	0.0	0.0	4.728885190543438	0.0
23	0.0	0.0	0.0	4.987759780953597	0.0
24	0.0	0.0	0.0	5.182153104596038	0.0
25	0.0	0.0	0.0	5.355656914774237	0.0
26	0.0	0.0	0.0	5.523031983398879	0.0
27	0.0	0.0	0.0	5.72787006377344	0.0
28	0.0	0.0	0.0	5.918896895576602	0.0
29	0.0	0.0	0.0	6.141430638183269	0.0
30	0.0	0.0	0.0	6.381401082111325	0.0
31	0.0	0.0	0.0	6.592540544646587	0.0
32	0.0	0.0	0.0	6.783653696753321	0.0
33	0.0	0.0	0.0	6.996605885663579	0.0
34	0.0	0.0	0.0	7.221297635859526	0.0
35	0.0	0.0	0.0	7.465584094966145	0.0
36	0.0	0.0	0.0	7.688894720304952	0.0
37	0.0	0.0	0.0	7.934648624572283	0.0
38	0.0	0.0	0.0	8.213894806625255	0.0
39	0.0	0.0	0.0	8.665867916124288	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAC	30	2.165516E-6	45.000004	38
GTCATCG	30	2.165516E-6	45.000004	1
CGGCGTA	30	2.165516E-6	45.000004	35
TTTCGCG	30	2.165516E-6	45.000004	1
ACTAGGT	30	2.165516E-6	45.000004	43
CGGGTTA	20	7.0333114E-4	45.0	6
GTTTCGC	20	7.0333114E-4	45.0	17
CGCATCG	35	1.211938E-7	45.0	21
GGCGTAG	100	0.0	45.0	1
CTAAGCG	45	3.8562575E-10	45.0	1
TCGGATA	25	3.890825E-5	45.0	39
CCATACG	55	1.8189894E-12	45.0	2
GTCGTAC	20	7.0333114E-4	45.0	30
TATAGCG	80	0.0	45.0	1
TTACGAT	20	7.0333114E-4	45.0	10
CCGCGTT	20	7.0333114E-4	45.0	45
CACGTTA	20	7.0333114E-4	45.0	40
CGTCCTA	20	7.0333114E-4	45.0	40
CGATAAT	25	3.890825E-5	45.0	43
GTTGCGC	25	3.890825E-5	45.0	35
>>END_MODULE