FastQCFastQC Report
Thu 26 May 2016
SRR1547568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547568_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1549377
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53430.34484828418131935No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG32310.20853543069246544No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22840.14741408966313557No Hit
AGGCAGGAGACTCAGCCCGAGGAAATCGCAGATAAGTTTTTAATTAAAAAG16740.10804342648690408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT551.8189894E-1245.0000046
CTCGCGC2200.045.00000433
GCCCCCG207.0340483E-445.012
CTAGACG1000.045.01
TTAGGCG351.2123019E-745.01
AGCCGAT351.2123019E-745.038
CGAACGC207.0340483E-445.025
CGAACCA207.0340483E-445.015
CGAACAC351.2123019E-745.028
TCCGCAA351.2123019E-745.022
CTCGTAG253.8914368E-545.01
ACGCATA253.8914368E-545.035
TCCGATC207.0340483E-445.017
CGTGCGT351.2123019E-745.030
ACGTTAG502.1827873E-1145.01
CCGATAT253.8914368E-545.011
AACCCGC406.8175723E-945.019
GCGCGAT253.8914368E-545.040
CATGACG207.0340483E-445.030
CGTTGCG207.0340483E-445.015