FastQCFastQC Report
Thu 26 May 2016
SRR1547567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547567_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1885982
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167730.889351011833623No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT21450.11373385323932043TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAG453.8562575E-1045.0000041
ACGACGT453.8562575E-1045.00000415
ACCGGTG253.89176E-545.014
CGACGTG253.89176E-545.016
GCGCGGT253.89176E-545.029
CCGGCCC253.89176E-545.040
TAACGCC551.8189894E-1245.012
CGTTCCG207.0344395E-445.028
TCTAGCG551.8189894E-1245.01
CCCCGTA207.0344395E-445.018
CTACGCG351.2124838E-745.01
ATCCCGG207.0344395E-445.027
GTCGTAC253.89176E-545.044
TTAACGC351.2124838E-745.021
TCCCGGT207.0344395E-445.028
ATGCGAT253.89176E-545.01
ACCGTAG253.89176E-545.036
CGATATC406.8193913E-945.010
ATCGCTT253.89176E-545.025
CGCCCGA207.0344395E-445.033