##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547567_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1885982
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.358318902301296	31.0	31.0	33.0	30.0	34.0
2	31.766867340197308	31.0	31.0	34.0	30.0	34.0
3	31.8902889847305	31.0	31.0	34.0	30.0	34.0
4	35.64496214704064	37.0	35.0	37.0	33.0	37.0
5	35.54485938890191	37.0	35.0	37.0	33.0	37.0
6	35.58897062644288	37.0	35.0	37.0	33.0	37.0
7	35.98109048760805	37.0	35.0	37.0	35.0	37.0
8	35.94248513506491	37.0	35.0	37.0	35.0	37.0
9	37.611257689628005	39.0	37.0	39.0	35.0	39.0
10	37.13456491101188	39.0	37.0	39.0	33.0	39.0
11	36.79257861421795	39.0	37.0	39.0	32.0	39.0
12	36.51597417154564	39.0	35.0	39.0	32.0	39.0
13	36.43999465530424	39.0	35.0	39.0	32.0	39.0
14	37.523747310419715	40.0	36.0	41.0	32.0	41.0
15	37.72705306837499	40.0	36.0	41.0	33.0	41.0
16	37.77815270771407	40.0	36.0	41.0	33.0	41.0
17	37.706301014537786	40.0	36.0	41.0	33.0	41.0
18	37.62898479412847	39.0	36.0	41.0	33.0	41.0
19	37.606973449375445	39.0	36.0	41.0	32.0	41.0
20	37.493117113524946	39.0	35.0	41.0	32.0	41.0
21	37.36561377574123	39.0	35.0	41.0	32.0	41.0
22	37.27372689665119	39.0	35.0	41.0	32.0	41.0
23	37.18173238132707	39.0	35.0	41.0	32.0	41.0
24	37.12676367006684	39.0	35.0	41.0	32.0	41.0
25	37.06682089224606	39.0	35.0	41.0	32.0	41.0
26	37.05905252542177	39.0	35.0	41.0	32.0	41.0
27	37.00661353077601	39.0	35.0	41.0	31.0	41.0
28	36.950842584923926	39.0	35.0	41.0	31.0	41.0
29	36.919497641016726	39.0	35.0	41.0	31.0	41.0
30	36.80949075866047	39.0	35.0	41.0	31.0	41.0
31	36.69945524400551	39.0	35.0	41.0	31.0	41.0
32	36.563294347454004	39.0	35.0	41.0	30.0	41.0
33	36.43918976957362	39.0	35.0	41.0	30.0	41.0
34	36.37404863885233	39.0	35.0	41.0	30.0	41.0
35	36.266302647639264	39.0	35.0	41.0	30.0	41.0
36	36.193769611799055	39.0	35.0	41.0	30.0	41.0
37	36.16421471678945	39.0	35.0	41.0	30.0	41.0
38	36.06536064501146	38.0	35.0	41.0	30.0	41.0
39	36.0124937565682	38.0	35.0	41.0	29.0	41.0
40	35.965184185214916	38.0	35.0	41.0	29.0	41.0
41	35.90726210536474	38.0	35.0	41.0	29.0	41.0
42	35.867891634172544	38.0	35.0	40.0	29.0	41.0
43	35.818179600865754	38.0	35.0	40.0	29.0	41.0
44	35.71891195143962	38.0	35.0	40.0	28.0	41.0
45	35.65868603199819	38.0	35.0	40.0	28.0	41.0
46	35.5955194694329	38.0	35.0	40.0	28.0	41.0
47	35.531805711825456	38.0	34.0	40.0	27.0	41.0
48	35.4345094491888	38.0	34.0	40.0	27.0	41.0
49	35.375601675943884	38.0	34.0	40.0	27.0	41.0
50	35.28697304640235	38.0	34.0	40.0	27.0	41.0
51	34.842671881279884	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	0.0
11	2.0
12	3.0
13	8.0
14	19.0
15	31.0
16	104.0
17	248.0
18	553.0
19	1056.0
20	1921.0
21	3091.0
22	5040.0
23	7702.0
24	11506.0
25	17134.0
26	22540.0
27	26741.0
28	29898.0
29	34406.0
30	41494.0
31	51401.0
32	64025.0
33	82255.0
34	129986.0
35	181317.0
36	147194.0
37	181545.0
38	279747.0
39	564836.0
40	177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.46489096926694	23.911999160119237	30.141273882783608	13.481835987830213
2	29.926531642401677	25.703426649883195	31.042555019082897	13.327486688632234
3	27.533984947894517	25.898232326713615	32.961661352017146	13.60612137337472
4	26.450570578086115	27.780063648539592	31.7120205813205	14.057345192053795
5	23.1608785237611	31.47935664285237	30.875162117135798	14.484602716250738
6	22.121154920884717	40.10748777029685	27.23472440352029	10.536632905298141
7	87.89102971290288	3.0274944299574438	7.146197577707529	1.9352782794321473
8	87.93392513820386	2.772348834718465	6.953247698016206	2.3404783290614652
9	82.9285221173903	5.583722432133499	8.545998848345318	2.941756602130879
10	41.82420616951805	36.20909425434601	13.275842505389765	8.690857070746167
11	26.351046828654777	25.8907561153818	30.372400160765057	17.385796895198364
12	26.73615124640638	24.452089150373652	30.466780700982298	18.344978902237667
13	24.052403469386242	27.41362324772983	30.294668772024337	18.239304510859593
14	16.23011248251574	32.36091330670176	31.178399369665243	20.230574841117253
15	16.195117450749798	32.34574879293652	34.799271679157066	16.659862077156625
16	20.450460290713274	30.695096771867387	32.955245596193386	15.899197341225952
17	20.310480163649494	30.491860473747895	28.621906253612178	20.575753108990437
18	20.342718011094483	31.228081710217808	30.94584147674792	17.483358801939787
19	21.99973276521197	32.27565268385382	28.00026723478803	17.724347316146176
20	23.719208348754123	31.80767366814742	28.19979193862932	16.27332604446914
21	23.3516544696609	29.63978447302254	30.864769653156817	16.143791404159742
22	22.54820035398005	25.417792958787516	31.021080795044703	21.01292589218773
23	18.21655774021173	29.326631961492737	32.28493166954934	20.171878628746192
24	18.520961493800048	29.714917745768517	33.61018291797058	18.153937842460852
25	19.1175207398586	31.538530060202056	30.02838839395074	19.315560805988603
26	18.955483138227194	34.88363091482316	27.38175655971266	18.779129387236992
27	18.03034175299658	34.520477926088375	30.46821231591818	16.980968004996868
28	17.36273198789808	31.871884249160388	32.593630267945294	18.17175349499624
29	17.87901475199657	29.196620116204713	33.1912499695119	19.73311516228681
30	19.8787687263187	30.523515070663453	30.70320925650404	18.894506946513808
31	21.985469638628576	30.11826199825873	28.932248558045625	18.964019805067068
32	22.74438462297095	30.863285015445534	28.34899802861321	18.043332332970305
33	21.194422852392016	30.41545465439225	28.926628143852913	19.463494349362826
34	19.699392677130533	29.62366554930005	29.401765234238713	21.275176539330705
35	19.486506233887706	30.33941999446442	29.20441446418895	20.969659307458926
36	21.06382775657456	30.788310811025767	29.040574088193843	19.10728734420583
37	19.154530637089856	31.837896650127096	30.105642577712832	18.90193013507022
38	18.549169610314415	33.030484914490174	28.350482666324496	20.069862808870923
39	18.880986138786053	31.662603354644954	28.78998845163952	20.666422054929473
40	20.312495029114803	29.416081383597508	29.730718532838594	20.540705054449088
41	18.50966764263922	29.001867462149693	30.00627789660771	22.482186998603378
42	20.196958401511786	29.03230253523098	29.245188978473813	21.52555008478342
43	20.838268870010424	29.456855897882377	28.61082449355296	21.094050738554238
44	19.416357102029604	30.48963351718097	28.76612820270819	21.32788117808123
45	19.339261986593723	31.68025993885413	28.101275621930643	20.8792024526215
46	21.020720240171965	30.536823787289592	28.215221566271577	20.227234406266867
47	19.76317907594028	29.88178041996159	28.992164294250955	21.36287620984718
48	19.103416681601416	29.742807725630467	30.61487331268273	20.538902280085388
49	20.59940126682015	29.15229307596785	30.096575683118925	20.151729974093072
50	19.26847658143079	29.53538262825414	30.70161857324195	20.494522217073122
51	18.18490314329617	30.46895463477382	29.177531917059653	22.168610304870352
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1366.0
1	2027.0
2	2688.0
3	12740.5
4	22793.0
5	15206.5
6	7620.0
7	7995.0
8	8370.0
9	9617.0
10	10864.0
11	12233.5
12	13603.0
13	13728.5
14	13854.0
15	13333.5
16	12813.0
17	11805.5
18	10798.0
19	10326.0
20	9854.0
21	10857.5
22	11861.0
23	13621.5
24	15382.0
25	17819.0
26	23131.0
27	26006.0
28	33998.5
29	41991.0
30	47023.5
31	52056.0
32	58192.0
33	64328.0
34	69292.0
35	74256.0
36	81448.0
37	88640.0
38	94109.5
39	99579.0
40	113045.0
41	126511.0
42	137413.0
43	148315.0
44	152817.5
45	157320.0
46	162101.0
47	166882.0
48	173812.0
49	180742.0
50	162397.0
51	144052.0
52	124104.5
53	104157.0
54	88857.5
55	73558.0
56	66406.0
57	59254.0
58	52412.0
59	45570.0
60	38402.0
61	31234.0
62	24995.0
63	18756.0
64	14609.5
65	10463.0
66	7533.0
67	4603.0
68	3660.5
69	2718.0
70	2047.0
71	1376.0
72	1075.0
73	774.0
74	604.0
75	278.5
76	123.0
77	94.5
78	66.0
79	61.5
80	57.0
81	39.0
82	21.0
83	13.5
84	6.0
85	3.5
86	1.0
87	2.5
88	4.0
89	3.5
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1885982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.799457240698892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28035044413764	15.65790430127669
2	10.072493258217467	4.190047856630441
3	3.9866980518215342	2.487634669813187
4	2.055600472054425	1.7102149648982587
5	1.2219193799224224	1.2707629947138865
6	0.7882004479294568	0.983648490828507
7	0.5669066673718005	0.8253945691226813
8	0.4248953466035132	0.7070074074761368
9	0.31857062469115527	0.5963486477765777
>10	3.0689448427783055	14.481412613752195
>50	1.0233820538235825	15.507572568257908
>100	1.1855907775360721	39.42206559432236
>500	0.0036106745430850877	0.5385211503212666
>1k	0.002579053245060777	0.7217116800898815
>5k	0.0	0.0
>10k+	2.579053245060777E-4	0.8997524907200523
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16773	0.889351011833623	No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	2145	0.11373385323932043	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0604555080589317E-4	0.0	0.0	0.124073294442895	0.0
2	1.0604555080589317E-4	0.0	0.0	0.37725704699196494	0.0
3	1.0604555080589317E-4	0.0	0.0	0.5741836348385085	0.0
4	1.0604555080589317E-4	0.0	0.0	0.8386082157730032	0.0
5	1.0604555080589317E-4	0.0	0.0	1.534638188487483	0.0
6	1.0604555080589317E-4	0.0	0.0	2.2339025505015426	0.0
7	1.0604555080589317E-4	0.0	0.0	2.744405832081112	0.0
8	1.0604555080589317E-4	0.0	0.0	3.6910214413499176	0.0
9	1.0604555080589317E-4	0.0	0.0	4.02379237977881	0.0
10	1.0604555080589317E-4	0.0	0.0	4.56038286685663	0.0
11	1.0604555080589317E-4	0.0	0.0	5.384621910495434	0.0
12	1.0604555080589317E-4	0.0	0.0	5.998731695212362	0.0
13	1.0604555080589317E-4	0.0	0.0	6.228638449359538	0.0
14	1.0604555080589317E-4	0.0	0.0	6.323549217330812	0.0
15	1.0604555080589317E-4	0.0	0.0	6.4649079365550675	0.0
16	1.0604555080589317E-4	0.0	0.0	6.769099599041772	0.0
17	1.0604555080589317E-4	0.0	0.0	7.173239193163031	0.0
18	1.0604555080589317E-4	0.0	0.0	7.5700086215032805	0.0
19	1.0604555080589317E-4	0.0	0.0	7.858770656347728	0.0
20	1.0604555080589317E-4	0.0	0.0	8.144298301892595	0.0
21	1.0604555080589317E-4	0.0	0.0	8.499869033744755	0.0
22	1.0604555080589317E-4	0.0	0.0	8.88179208497218	0.0
23	1.0604555080589317E-4	0.0	0.0	9.277554080579772	0.0
24	1.0604555080589317E-4	0.0	0.0	9.564460318285116	0.0
25	1.0604555080589317E-4	0.0	0.0	9.817325934181769	0.0
26	1.0604555080589317E-4	0.0	0.0	10.050042895425301	0.0
27	1.0604555080589317E-4	0.0	0.0	10.286047268743816	0.0
28	1.0604555080589317E-4	0.0	0.0	10.537375224153783	0.0
29	1.0604555080589317E-4	0.0	0.0	10.814366202858777	0.0
30	1.0604555080589317E-4	0.0	0.0	11.14215300039979	0.0
31	1.0604555080589317E-4	0.0	0.0	11.435368948378086	0.0
32	1.0604555080589317E-4	0.0	0.0	11.698998187681537	0.0
33	1.0604555080589317E-4	0.0	0.0	11.958226536626542	0.0
34	1.0604555080589317E-4	0.0	0.0	12.236755175818221	0.0
35	1.0604555080589317E-4	0.0	0.0	12.544499364256923	0.0
36	1.0604555080589317E-4	0.0	0.0	12.822921957897796	0.0
37	1.0604555080589317E-4	0.0	0.0	13.115554655346658	0.0
38	1.0604555080589317E-4	0.0	0.0	13.41126267376889	0.0
39	1.0604555080589317E-4	0.0	0.0	13.771605455407316	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAG	45	3.8562575E-10	45.000004	1
ACGACGT	45	3.8562575E-10	45.000004	15
ACCGGTG	25	3.89176E-5	45.0	14
CGACGTG	25	3.89176E-5	45.0	16
GCGCGGT	25	3.89176E-5	45.0	29
CCGGCCC	25	3.89176E-5	45.0	40
TAACGCC	55	1.8189894E-12	45.0	12
CGTTCCG	20	7.0344395E-4	45.0	28
TCTAGCG	55	1.8189894E-12	45.0	1
CCCCGTA	20	7.0344395E-4	45.0	18
CTACGCG	35	1.2124838E-7	45.0	1
ATCCCGG	20	7.0344395E-4	45.0	27
GTCGTAC	25	3.89176E-5	45.0	44
TTAACGC	35	1.2124838E-7	45.0	21
TCCCGGT	20	7.0344395E-4	45.0	28
ATGCGAT	25	3.89176E-5	45.0	1
ACCGTAG	25	3.89176E-5	45.0	36
CGATATC	40	6.8193913E-9	45.0	10
ATCGCTT	25	3.89176E-5	45.0	25
CGCCCGA	20	7.0344395E-4	45.0	33
>>END_MODULE