FastQCFastQC Report
Thu 26 May 2016
SRR1547566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547566_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1418661
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46460.327491909624639No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16420.11574294352209584No Hit
GGGAATGGTTACATCAGTGCGGCAGAACTGCGCCACGTCATGACAAACTTA15300.10784817514543643No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG14270.1005878077990443No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA302.1658634E-645.00000423
AGCCGTA351.2121927E-745.00000432
TCGTTGA253.8912687E-545.00000424
TCGTTAA351.2121927E-745.00000411
CTCGTTA253.8912687E-545.00000411
GTCGACG351.2121927E-745.00000424
CACGGCG253.8912687E-545.00000417
CGTGCCG253.8912687E-545.00000429
TTTCGCG302.1658634E-645.0000041
CGTTGTA253.8912687E-545.00000433
ATATGCG302.1658634E-645.0000041
TAACGAC253.8912687E-545.00000437
CCTCGGT253.8912687E-545.00000421
ACGGGTC700.045.0000045
AATGCGC302.1658634E-645.00000437
ATATCGT302.1658634E-645.00000418
ACGATAC253.8912687E-545.00000426
TGACCGG700.045.0000042
TATCTCG253.8912687E-545.0000041
CGCTATA351.2121927E-745.00000442