##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547566_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1418661
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.436095726886126	31.0	31.0	33.0	30.0	34.0
2	31.860008839321022	31.0	31.0	34.0	30.0	34.0
3	32.0243031985795	33.0	31.0	34.0	30.0	34.0
4	35.750257461084786	37.0	35.0	37.0	35.0	37.0
5	35.65564994033106	37.0	35.0	37.0	33.0	37.0
6	35.70430497490239	37.0	35.0	37.0	33.0	37.0
7	35.96366855788663	37.0	35.0	37.0	35.0	37.0
8	35.88816073748415	37.0	35.0	37.0	35.0	37.0
9	37.52721545175345	39.0	37.0	39.0	35.0	39.0
10	37.18558415294422	39.0	37.0	39.0	33.0	39.0
11	36.95598525652006	39.0	37.0	39.0	33.0	39.0
12	36.458564096708095	39.0	35.0	39.0	32.0	39.0
13	36.24738750131286	39.0	35.0	39.0	32.0	39.0
14	37.16105256999382	39.0	35.0	41.0	31.0	41.0
15	37.44221487726807	40.0	35.0	41.0	32.0	41.0
16	37.55369817031694	40.0	35.0	41.0	33.0	41.0
17	37.50171464500681	39.0	35.0	41.0	33.0	41.0
18	37.457739375368746	39.0	36.0	41.0	32.0	41.0
19	37.45895390089669	39.0	35.0	41.0	32.0	41.0
20	37.33885473696676	39.0	35.0	41.0	32.0	41.0
21	37.19769839306219	39.0	35.0	41.0	32.0	41.0
22	37.1693188154182	39.0	35.0	41.0	32.0	41.0
23	37.10118062031733	39.0	35.0	41.0	32.0	41.0
24	37.022943465704635	39.0	35.0	41.0	32.0	41.0
25	36.882121239676	39.0	35.0	41.0	31.0	41.0
26	36.927702248810675	39.0	35.0	41.0	32.0	41.0
27	36.94467529593046	39.0	35.0	41.0	32.0	41.0
28	36.91952975376076	39.0	35.0	41.0	31.0	41.0
29	36.89503834954228	39.0	35.0	41.0	31.0	41.0
30	36.81605683105407	39.0	35.0	41.0	31.0	41.0
31	36.655314412675054	39.0	35.0	41.0	31.0	41.0
32	36.56762820716154	39.0	35.0	41.0	31.0	41.0
33	36.48240136297537	39.0	35.0	41.0	30.0	41.0
34	36.41563347409987	39.0	35.0	41.0	30.0	41.0
35	36.329168138124615	39.0	35.0	41.0	30.0	41.0
36	36.248659122933525	39.0	35.0	41.0	30.0	41.0
37	36.21898466229776	39.0	35.0	41.0	30.0	41.0
38	36.0962837492537	38.0	35.0	41.0	30.0	41.0
39	36.041805618114545	38.0	35.0	41.0	29.0	41.0
40	35.97025927970107	38.0	35.0	41.0	29.0	41.0
41	35.897051515478324	38.0	35.0	40.0	29.0	41.0
42	35.90188071709873	38.0	35.0	40.0	29.0	41.0
43	35.882311559985084	38.0	35.0	40.0	29.0	41.0
44	35.78371577142108	38.0	35.0	40.0	29.0	41.0
45	35.73968270080026	38.0	35.0	40.0	29.0	41.0
46	35.70389472890282	38.0	35.0	40.0	29.0	41.0
47	35.6622470061558	38.0	35.0	40.0	29.0	41.0
48	35.57697363922741	38.0	34.0	40.0	29.0	41.0
49	35.51535990627782	38.0	34.0	40.0	28.0	41.0
50	35.428196729169265	38.0	34.0	40.0	28.0	41.0
51	34.99475702792986	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	9.0
11	12.0
12	12.0
13	12.0
14	14.0
15	44.0
16	93.0
17	235.0
18	459.0
19	910.0
20	1571.0
21	2466.0
22	3798.0
23	5791.0
24	8361.0
25	11976.0
26	15995.0
27	18999.0
28	21699.0
29	25019.0
30	30654.0
31	37991.0
32	48185.0
33	63283.0
34	109284.0
35	150367.0
36	97668.0
37	131111.0
38	211222.0
39	421275.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.526528183970655	24.44452903124848	27.60892137022164	12.420021414559223
2	32.51580187232891	24.814525809901024	28.140267477572163	14.529404840197904
3	30.814408798155448	25.357150157789633	29.832356003301708	13.996085040753217
4	28.57821565546667	27.227787329037735	28.4683232992237	15.725673716271892
5	24.775192946024454	31.8503856805819	27.931620027617594	15.442801345776052
6	22.899903500554398	39.889586025132154	25.857410614657063	11.353099859656394
7	86.31159945892641	4.25443428697906	6.788654935886727	2.645311318207803
8	85.6929174764091	4.631057031947731	6.827000953716215	2.8490245379269608
9	80.26653301951629	7.039102364835574	8.658375750091107	4.035988865557029
10	40.572483489713186	34.617149551584205	14.748837107667018	10.061529851035589
11	33.592662376705924	24.11675516561039	26.10235990134359	16.188222556340097
12	33.15450273180133	22.548868263806504	28.107560580011715	16.189068424380455
13	26.5208531143099	27.90871110152461	27.281006526576824	18.28942925758867
14	18.388607285320454	32.554993758198755	29.520371674416936	19.536027282063863
15	17.785009949522827	29.717106482803153	36.252917363626686	16.244966204047337
16	23.39198723303171	26.672052026523602	33.92170504440455	16.014255696040138
17	23.590272799491917	26.686079338192847	27.08892399241256	22.634723869902675
18	23.168537092370904	28.43385417657918	29.701457924056555	18.696150806993355
19	26.69052014540472	29.27041766849163	25.74180864914169	18.297253536961968
20	27.99463719662414	29.33103821138383	26.849261380978263	15.825063211013765
21	25.90090232973205	26.98481173444537	30.36574629174976	16.748539644072828
22	23.8942213819933	23.537969958996545	31.303250036478058	21.264558622532093
23	22.287142594319572	26.901423243466905	30.415300061113964	20.39613410109956
24	20.518432521934415	28.171000683038443	32.85591131355553	18.45465548147161
25	21.418647583883676	28.414328722647625	30.136304585803092	20.03071910766561
26	19.90123080848772	32.91434669734348	26.78349514084055	20.400927353328242
27	18.43534149455014	33.292802156399596	30.25444415543953	18.017412193610735
28	17.202065891710564	29.12859379372521	34.14374540499809	19.52559490956613
29	19.160250405135546	28.510828168251614	33.12912669059063	19.19979473602221
30	22.028236484967163	29.23834517196145	30.241756134834187	18.4916622082372
31	24.726837489717415	27.727483873878256	27.903706382285833	19.641972254118496
32	26.21443741669081	28.699104296234268	27.368624357757064	17.717833929317855
33	25.194461538027763	27.89278058676456	27.009341907615703	19.903415967591975
34	20.374212021053655	28.193134230094437	30.160552802959973	21.272100945891935
35	20.81646002815331	29.254064219711406	29.070299387943983	20.859176364191303
36	25.000616778779428	27.711059936094667	28.352227910684796	18.936095374441113
37	21.27541392905	30.91111971076952	28.405799553240698	19.407666806939783
38	20.802221249473977	31.31234311791189	27.1115509624921	20.773884670122037
39	20.148858677302048	29.92554246574763	28.975209722407257	20.950389134543066
40	23.670277818308953	27.865360364456343	27.31843618736259	21.14592562987211
41	18.769952793514445	27.506712315345244	29.50141013251228	24.22192475862803
42	20.40135028734842	26.433235283129658	29.129369172762203	24.03604525675972
43	21.029407307313022	27.197406568588267	28.30944108564343	23.463745038455276
44	20.15957300581323	28.749715400648924	29.05613109826801	22.034580495269836
45	19.74044539181665	31.24051482348496	26.579077031087767	22.439962753610622
46	20.87447247792108	30.72298456079359	27.808264271732284	20.594278689553036
47	20.089295469460286	29.220440965107237	29.330121854340113	21.36014171109236
48	20.493338436737176	28.76973427760402	30.071313724702375	20.665613560956423
49	21.752694970821075	26.780816558712754	30.37371154912978	21.092776921336387
50	19.350782181225817	29.417457729506907	29.780687563836604	21.45107252543067
51	18.364077112150117	30.84013728438295	28.64017548942277	22.15561011404416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1108.0
1	2041.0
2	2974.0
3	5900.5
4	8827.0
5	6525.5
6	4224.0
7	4305.0
8	4386.0
9	4616.0
10	4846.0
11	4755.5
12	4665.0
13	4709.0
14	4753.0
15	4546.5
16	4340.0
17	4374.5
18	4409.0
19	4750.0
20	5091.0
21	6009.5
22	6928.0
23	8102.5
24	9277.0
25	11159.5
26	17228.0
27	21414.0
28	22745.5
29	24077.0
30	28595.0
31	33113.0
32	38558.5
33	44004.0
34	46149.5
35	48295.0
36	54998.5
37	61702.0
38	67036.0
39	72370.0
40	85766.5
41	99163.0
42	102032.5
43	104902.0
44	112449.0
45	119996.0
46	118746.5
47	117497.0
48	115673.5
49	113850.0
50	106553.0
51	99256.0
52	94871.5
53	90487.0
54	81580.5
55	72674.0
56	65355.0
57	58036.0
58	56317.5
59	54599.0
60	46570.0
61	38541.0
62	33186.5
63	27832.0
64	22097.0
65	16362.0
66	13211.0
67	10060.0
68	7633.0
69	5206.0
70	4205.5
71	3205.0
72	2352.0
73	1499.0
74	1135.5
75	699.5
76	627.0
77	399.0
78	171.0
79	100.0
80	29.0
81	27.0
82	25.0
83	16.5
84	8.0
85	8.0
86	8.0
87	5.5
88	3.0
89	4.0
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1418661.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.727547211828954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62221726565157	12.365346339442087
2	8.469310414270678	2.6640295878415348
3	2.7650306140101786	1.3046144857199247
4	1.4209147757028953	0.8939001687541054
5	0.8456036001658082	0.6649635272050142
6	0.5374564175735985	0.5071722709013536
7	0.4470880850410138	0.492211927573012
8	0.3602721876205374	0.4532958271928721
9	0.30036405710700304	0.4251590899998191
>10	2.927913257085175	10.838342184100044
>50	1.3238893414706911	15.229096774599778
>100	1.973592569650003	52.999213428883266
>500	0.004080480846967614	0.425843115845764
>1k	0.002266933803870897	0.73681127194144
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4646	0.327491909624639	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1642	0.11574294352209584	No Hit
GGGAATGGTTACATCAGTGCGGCAGAACTGCGCCACGTCATGACAAACTTA	1530	0.10784817514543643	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1427	0.1005878077990443	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.409780067260607E-4	0.0	0.0	0.045817852185969725	0.0
2	1.409780067260607E-4	0.0	0.0	0.13886333662516978	0.0
3	1.409780067260607E-4	0.0	0.0	0.19469062728868983	0.0
4	1.409780067260607E-4	0.0	0.0	0.29527843508773416	0.0
5	1.409780067260607E-4	0.0	0.0	0.5020931709548652	0.0
6	1.409780067260607E-4	0.0	0.0	0.6677423288579865	0.0
7	1.409780067260607E-4	0.0	0.0	0.7771412620774096	0.0
8	1.409780067260607E-4	0.0	0.0	1.031254119201134	0.0
9	1.409780067260607E-4	0.0	0.0	1.1013906775473492	0.0
10	1.409780067260607E-4	0.0	0.0	1.273101889739691	0.0
11	1.409780067260607E-4	0.0	0.0	1.5695081488812337	0.0
12	1.409780067260607E-4	0.0	0.0	1.8004301238985212	0.0
13	1.409780067260607E-4	0.0	0.0	1.8841005708904381	0.0
14	1.409780067260607E-4	0.0	0.0	1.9245612588208176	0.0
15	1.409780067260607E-4	0.0	0.0	1.9931470590930462	0.0
16	1.409780067260607E-4	0.0	0.0	2.139200274061245	0.0
17	1.409780067260607E-4	0.0	0.0	2.3092902391762373	0.0
18	1.409780067260607E-4	0.0	0.0	2.48938964276878	0.0
19	1.409780067260607E-4	0.0	0.0	2.6159174038054194	0.0
20	1.409780067260607E-4	0.0	0.0	2.7560495424911235	0.0
21	1.409780067260607E-4	0.0	0.0	2.9273378206632876	0.0
22	1.409780067260607E-4	0.0	0.0	3.114979547615674	0.0
23	1.409780067260607E-4	0.0	0.0	3.2938101491476823	0.0
24	1.409780067260607E-4	0.0	0.0	3.42703436550381	0.0
25	1.409780067260607E-4	0.0	0.0	3.548980341321852	0.0
26	1.409780067260607E-4	0.0	0.0	3.674450767308046	0.0
27	1.409780067260607E-4	0.0	0.0	3.834037870921947	0.0
28	1.409780067260607E-4	0.0	0.0	3.9771305477488985	0.0
29	2.1146701008909106E-4	0.0	0.0	4.159556088452421	0.0
30	2.1146701008909106E-4	0.0	0.0	4.371375543558327	0.0
31	2.819560134521214E-4	0.0	0.0	4.563246610712496	0.0
32	2.819560134521214E-4	0.0	0.0	4.724102516386931	0.0
33	2.819560134521214E-4	0.0	0.0	4.8836896200008315	0.0
34	2.819560134521214E-4	0.0	0.0	5.082468609484577	0.0
35	2.819560134521214E-4	0.0	0.0	5.2893538343550714	0.0
36	2.819560134521214E-4	0.0	0.0	5.4679024798736275	0.0
37	2.819560134521214E-4	0.0	0.0	5.676549929828197	0.0
38	2.819560134521214E-4	0.0	0.0	5.9252351336929685	0.0
39	2.819560134521214E-4	0.0	0.0	6.477445986038948	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	30	2.1658634E-6	45.000004	23
AGCCGTA	35	1.2121927E-7	45.000004	32
TCGTTGA	25	3.8912687E-5	45.000004	24
TCGTTAA	35	1.2121927E-7	45.000004	11
CTCGTTA	25	3.8912687E-5	45.000004	11
GTCGACG	35	1.2121927E-7	45.000004	24
CACGGCG	25	3.8912687E-5	45.000004	17
CGTGCCG	25	3.8912687E-5	45.000004	29
TTTCGCG	30	2.1658634E-6	45.000004	1
CGTTGTA	25	3.8912687E-5	45.000004	33
ATATGCG	30	2.1658634E-6	45.000004	1
TAACGAC	25	3.8912687E-5	45.000004	37
CCTCGGT	25	3.8912687E-5	45.000004	21
ACGGGTC	70	0.0	45.000004	5
AATGCGC	30	2.1658634E-6	45.000004	37
ATATCGT	30	2.1658634E-6	45.000004	18
ACGATAC	25	3.8912687E-5	45.000004	26
TGACCGG	70	0.0	45.000004	2
TATCTCG	25	3.8912687E-5	45.000004	1
CGCTATA	35	1.2121927E-7	45.000004	42
>>END_MODULE