FastQCFastQC Report
Thu 26 May 2016
SRR1547565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547565_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2436356
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177120.726987353243943No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37450.15371316835470678No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33620.1379929698287114No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25830.10601898901474169No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATA453.8562575E-1045.00000432
GCCCCGA253.8920964E-545.032
CGAACAC406.8212103E-945.028
CTCCGAT253.8920964E-545.018
GCGCGTT351.2126839E-745.09
GCGCGAT207.0348446E-445.09
TAGCGAA207.0348446E-445.043
TCGATAG1000.045.01
TCCGTAC253.8920964E-545.032
TACTCGC551.8189894E-1245.045
CGTCGCA207.0348446E-445.042
GACGCGT207.0348446E-445.09
ATACGCG351.2126839E-745.01
TGTAACG750.045.01
TCGCGTC207.0348446E-445.020
AGTCGTC253.8920964E-545.045
TAATCGC351.2126839E-745.016
CGCGGGC1050.044.9999964
GCGCATT302.1665073E-644.99999610
TATCGAT302.1665073E-644.99999612