##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547565_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2436356
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39085790418149	31.0	31.0	33.0	30.0	34.0
2	31.809982202929294	31.0	31.0	34.0	30.0	34.0
3	31.94173060094666	31.0	31.0	34.0	30.0	34.0
4	35.673108938102644	37.0	35.0	37.0	33.0	37.0
5	35.57528415387571	37.0	35.0	37.0	33.0	37.0
6	35.626878830515736	37.0	35.0	37.0	33.0	37.0
7	36.01115107972726	37.0	35.0	37.0	35.0	37.0
8	35.980898932668296	37.0	35.0	37.0	35.0	37.0
9	37.67215956945537	39.0	38.0	39.0	35.0	39.0
10	37.15772448689764	39.0	37.0	39.0	33.0	39.0
11	36.8606632199892	39.0	37.0	39.0	32.0	39.0
12	36.487859327618786	39.0	35.0	39.0	32.0	39.0
13	36.335467805197595	39.0	35.0	39.0	32.0	39.0
14	37.20950632830342	40.0	36.0	41.0	31.0	41.0
15	37.482972110808106	40.0	36.0	41.0	32.0	41.0
16	37.59617970444385	40.0	35.0	41.0	32.0	41.0
17	37.57189261339476	40.0	35.0	41.0	32.0	41.0
18	37.50383482545244	39.0	36.0	41.0	32.0	41.0
19	37.49971145431948	39.0	36.0	41.0	32.0	41.0
20	37.35516484454653	39.0	35.0	41.0	32.0	41.0
21	37.21636041695056	39.0	35.0	41.0	32.0	41.0
22	37.19892002646576	39.0	35.0	41.0	32.0	41.0
23	37.14836871130492	39.0	35.0	41.0	32.0	41.0
24	37.08185667447614	39.0	35.0	41.0	32.0	41.0
25	37.00273810559705	39.0	35.0	41.0	32.0	41.0
26	37.029208785579776	39.0	35.0	41.0	32.0	41.0
27	37.02389593310666	39.0	35.0	41.0	32.0	41.0
28	36.97310409480388	39.0	35.0	41.0	31.0	41.0
29	36.94006581960929	39.0	35.0	41.0	31.0	41.0
30	36.84958437929432	39.0	35.0	41.0	31.0	41.0
31	36.68936969802443	39.0	35.0	41.0	31.0	41.0
32	36.6001446422444	39.0	35.0	41.0	30.0	41.0
33	36.49392576454344	39.0	35.0	41.0	30.0	41.0
34	36.38731819159433	39.0	35.0	41.0	30.0	41.0
35	36.30584323473253	39.0	35.0	41.0	30.0	41.0
36	36.23920395869897	39.0	35.0	41.0	30.0	41.0
37	36.20905072986049	39.0	35.0	41.0	30.0	41.0
38	36.070769214351266	38.0	35.0	41.0	29.0	41.0
39	36.01776669747771	38.0	35.0	41.0	29.0	41.0
40	35.93375516550127	38.0	35.0	41.0	29.0	41.0
41	35.864358492765426	38.0	35.0	41.0	29.0	41.0
42	35.87454789037398	38.0	35.0	40.0	29.0	41.0
43	35.86954451648281	38.0	35.0	40.0	29.0	41.0
44	35.77439873319006	38.0	35.0	40.0	29.0	41.0
45	35.71123924418271	38.0	35.0	40.0	28.0	41.0
46	35.695381545225736	38.0	35.0	40.0	29.0	41.0
47	35.63219742927552	38.0	34.0	40.0	28.0	41.0
48	35.544660960877636	38.0	34.0	40.0	28.0	41.0
49	35.48954627320474	38.0	34.0	40.0	28.0	41.0
50	35.38783946188488	38.0	34.0	40.0	28.0	41.0
51	34.98344289586579	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	1.0
11	7.0
12	7.0
13	8.0
14	20.0
15	58.0
16	137.0
17	367.0
18	707.0
19	1462.0
20	2576.0
21	4230.0
22	6391.0
23	9868.0
24	14649.0
25	21307.0
26	28320.0
27	33657.0
28	38077.0
29	43941.0
30	53435.0
31	67241.0
32	84020.0
33	110273.0
34	178026.0
35	234058.0
36	178402.0
37	232957.0
38	361973.0
39	729933.0
40	245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.06085481760465	24.4949834917393	31.50795696523825	12.936204725417797
2	29.87022422010576	26.151432713445814	31.086343703465342	12.891999362983078
3	28.90230327587594	25.2647396357511	32.73392722574205	13.099029862630912
4	26.233974016933487	27.56062742883224	31.8603274726682	14.345071081566077
5	23.269628904807014	31.999100295687494	30.456222325472957	14.27504847403253
6	21.720881513210713	40.97664709098342	26.933009790030688	10.369461605775182
7	89.86502793516219	3.0217258889915923	5.818238385523298	1.295007790322925
8	90.84210189315519	2.0894729670048218	5.4923828865732265	1.576042253266764
9	85.94285892537872	4.875272743392181	6.9056000026268745	2.276268328602224
10	40.649847559223694	37.358251421385056	12.933085312655457	9.058815706735796
11	29.04304625432408	24.777167212016636	28.773463319810404	17.40632321384888
12	30.386856436415698	22.246625698379056	29.44885722776146	17.917660637443788
13	26.054361513670415	26.104272117867833	29.80685088714457	18.034515481317182
14	17.356740968889603	30.878861709865063	31.885118595147837	19.8792787260975
15	16.355163202750337	30.663663274168474	36.492244975693204	16.488928547387985
16	23.01703856086713	28.651231593412458	32.82783796785035	15.503891877870066
17	23.494103488981086	27.336234934467708	27.40855605666824	21.761105519882975
18	22.597272319808763	29.61640252902285	30.832727236906265	16.95359791426212
19	25.140168349781394	30.157210194240907	26.379888653382345	18.32273280259535
20	24.588976323657132	31.971518119683655	27.10034986676824	16.33915568989097
21	25.81753241316129	26.72162853047748	31.961626297634666	15.499212758726557
22	23.31592755738488	22.534350480800015	33.079443234075804	21.070278727739296
23	19.543982899050878	27.298884071129176	33.185667447614385	19.971465582205557
24	18.997264767546287	29.922925877827378	32.67080016220946	18.40900919241687
25	21.209133640568126	29.762604479805088	30.03686653346227	18.99139534616452
26	19.219522926862904	33.10320823393626	26.45906427467907	21.21820456452177
27	17.719290612701922	32.3351759759247	32.16644037242505	17.779093038948332
28	16.199069429919106	30.599304863492854	33.60834787691125	19.59327782967678
29	18.232803416249514	29.380394326609082	32.82525213884998	19.561550118291414
30	20.607086977436794	28.460126516814455	32.19270911147632	18.74007739427243
31	23.42461446520952	28.306290213745445	28.844635184677443	19.424460136367593
32	25.064071096342243	28.291719272552946	28.394454669186274	18.249754961918537
33	23.173378603126967	28.17470024906048	27.650762039701917	21.001159108110638
34	18.931100381060894	29.12172933676359	30.281740435305842	21.665429846869667
35	18.859107618098506	30.1192026124261	29.446394533475402	21.575295235999995
36	23.04195281806107	29.403092158945572	28.430615230286545	19.124339792706813
37	19.259377529392257	32.55640801262213	28.512622950012233	19.671591507973382
38	20.672184196398227	31.707353112599307	26.323410864422115	21.29705182658035
39	19.48721779575727	29.940862501210823	27.825038705345197	22.74688099768671
40	22.025106347348252	27.702848023852017	29.19084895639225	21.081196672407483
41	18.738312463367425	27.222335323737585	29.635898858787467	24.403453354107526
42	19.231425949245512	28.04425954170901	29.341524801794154	23.382789707251323
43	20.104984657414597	27.761624327479232	29.002370753699374	23.13102026140679
44	20.40592589917073	29.033072342465548	29.62017866026147	20.94082309810225
45	19.79304338118075	30.431554337707627	26.94868894365191	22.826713337459715
46	21.16870440937203	29.885164565441176	28.045983427709253	20.900147597477545
47	20.01792841440249	29.060120934707406	28.97515798183845	21.94679266905165
48	19.168463065331995	29.84313458295914	30.047948657749522	20.94045369395934
49	21.42925746483683	27.69857935375618	29.879746638011852	20.992416543395137
50	19.221287857767912	29.443644524855973	29.235669992398485	22.09939762497763
51	17.760417607279066	30.576853300584972	29.103997937903987	22.558731154231975
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1975.0
1	2858.5
2	3742.0
3	14221.0
4	24700.0
5	16522.5
6	8345.0
7	8362.5
8	8380.0
9	9379.5
10	10379.0
11	11181.5
12	11984.0
13	12340.5
14	12697.0
15	12395.0
16	12093.0
17	11941.5
18	11790.0
19	11692.0
20	11594.0
21	12345.5
22	13097.0
23	14990.5
24	16884.0
25	18897.0
26	26636.0
27	32362.0
28	38632.5
29	44903.0
30	53389.0
31	61875.0
32	67750.5
33	73626.0
34	80288.5
35	86951.0
36	93307.0
37	99663.0
38	113514.5
39	127366.0
40	141841.5
41	156317.0
42	177319.0
43	198321.0
44	200801.5
45	203282.0
46	204563.0
47	205844.0
48	215647.5
49	225451.0
50	207349.5
51	189248.0
52	169677.0
53	150106.0
54	132614.5
55	115123.0
56	104943.0
57	94763.0
58	87856.0
59	80949.0
60	67552.0
61	54155.0
62	43552.5
63	32950.0
64	25394.0
65	17838.0
66	13381.0
67	8924.0
68	6481.5
69	4039.0
70	2964.5
71	1890.0
72	1370.5
73	851.0
74	651.0
75	314.5
76	178.0
77	168.5
78	159.0
79	150.0
80	141.0
81	82.0
82	23.0
83	16.0
84	9.0
85	16.5
86	24.0
87	13.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2436356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.40991913074997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8932812245095	13.603693316489847
2	10.637781287727812	3.9168138647534856
3	4.169752502986186	2.3029441912565387
4	2.1531978499834925	1.5856079316280323
5	1.3256955451048924	1.2202973888688284
6	0.8652754470554542	0.9557790603668663
7	0.6079427868993568	0.7834524280057944
8	0.450163318278924	0.662997622811604
9	0.3661362173849536	0.6066484337606118
>10	3.2215414050837636	13.135446629842079
>50	0.9771509003533956	12.961900706985777
>100	1.3201113012663361	45.416522648167366
>500	0.008356564047913684	1.0641408117965057
>1k	0.0033877962356406825	1.0473011670195125
>5k	0.0	0.0
>10k+	2.258530823760455E-4	0.7364537982471656
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17712	0.726987353243943	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	3745	0.15371316835470678	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	3362	0.1379929698287114	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2583	0.10601898901474169	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07018678715261645	0.0
2	0.0	0.0	0.0	0.2179073994112519	0.0
3	0.0	0.0	0.0	0.3164562157582882	0.0
4	0.0	0.0	0.0	0.4778447813045384	0.0
5	0.0	0.0	0.0	0.8696183973113946	0.0
6	0.0	0.0	0.0	1.2276531015992738	0.0
7	0.0	0.0	0.0	1.4939934886363078	0.0
8	0.0	0.0	0.0	2.0644766200013462	0.0
9	0.0	0.0	0.0	2.258085435790172	0.0
10	0.0	0.0	0.0	2.625724647793672	0.0
11	0.0	0.0	0.0	3.2639318720252706	0.0
12	0.0	0.0	0.0	3.746086368330408	0.0
13	0.0	0.0	0.0	3.922251099592999	0.0
14	0.0	0.0	0.0	3.997978948889243	0.0
15	0.0	0.0	0.0	4.108841236666563	0.0
16	0.0	0.0	0.0	4.346162876032895	0.0
17	0.0	0.0	0.0	4.666066863791663	0.0
18	0.0	0.0	0.0	5.025784409175014	0.0
19	0.0	0.0	0.0	5.2806322228771165	0.0
20	0.0	0.0	0.0	5.543360658294601	0.0
21	0.0	0.0	0.0	5.874305725435856	0.0
22	4.1044904767611955E-5	0.0	0.0	6.259717381203732	0.0
23	4.1044904767611955E-5	0.0	0.0	6.617834175301146	0.0
24	4.1044904767611955E-5	0.0	0.0	6.870876013193474	0.0
25	4.1044904767611955E-5	0.0	0.0	7.09370880117684	0.0
26	4.1044904767611955E-5	0.0	0.0	7.310384853445063	0.0
27	4.1044904767611955E-5	0.0	0.0	7.57528866881523	0.0
28	4.1044904767611955E-5	0.0	0.0	7.837278295946898	0.0
29	4.1044904767611955E-5	0.0	0.0	8.144991946989684	0.0
30	4.1044904767611955E-5	0.0	0.0	8.507952039849677	0.0
31	4.1044904767611955E-5	0.0	0.0	8.810740302320351	0.0
32	4.1044904767611955E-5	0.0	0.0	9.073961276595046	0.0
33	4.1044904767611955E-5	0.0	0.0	9.350152440776307	0.0
34	4.1044904767611955E-5	0.0	0.0	9.64711232676998	0.0
35	4.1044904767611955E-5	0.0	0.0	9.983926815293003	0.0
36	4.1044904767611955E-5	0.0	0.0	10.281379240143886	0.0
37	4.1044904767611955E-5	0.0	0.0	10.612939980856657	0.0
38	4.1044904767611955E-5	0.0	0.0	11.028191282390587	0.0
39	4.1044904767611955E-5	0.0	0.0	11.862100612554158	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGATA	45	3.8562575E-10	45.000004	32
GCCCCGA	25	3.8920964E-5	45.0	32
CGAACAC	40	6.8212103E-9	45.0	28
CTCCGAT	25	3.8920964E-5	45.0	18
GCGCGTT	35	1.2126839E-7	45.0	9
GCGCGAT	20	7.0348446E-4	45.0	9
TAGCGAA	20	7.0348446E-4	45.0	43
TCGATAG	100	0.0	45.0	1
TCCGTAC	25	3.8920964E-5	45.0	32
TACTCGC	55	1.8189894E-12	45.0	45
CGTCGCA	20	7.0348446E-4	45.0	42
GACGCGT	20	7.0348446E-4	45.0	9
ATACGCG	35	1.2126839E-7	45.0	1
TGTAACG	75	0.0	45.0	1
TCGCGTC	20	7.0348446E-4	45.0	20
AGTCGTC	25	3.8920964E-5	45.0	45
TAATCGC	35	1.2126839E-7	45.0	16
CGCGGGC	105	0.0	44.999996	4
GCGCATT	30	2.1665073E-6	44.999996	10
TATCGAT	30	2.1665073E-6	44.999996	12
>>END_MODULE