FastQCFastQC Report
Thu 26 May 2016
SRR1547564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547564_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2128653
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82090.385642939455139No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT32620.15324244956787225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA551.8189894E-1245.0000049
TTAGCCG551.8189894E-1245.0000041
AGTTCGC453.8562575E-1045.00000415
CGCCGGT900.045.00000428
TCCGGCA207.034643E-445.036
AACGTCG253.89193E-545.022
AACCGTT502.1827873E-1145.029
TAATACG850.045.01
AAATCCG253.89193E-545.031
TCCGCGT207.034643E-445.040
GTCGACT502.1827873E-1145.035
GATCGCG207.034643E-445.09
CGAAACG253.89193E-545.019
ACGCACT207.034643E-445.010
CGCGCCA207.034643E-445.010
ATTCGAC253.89193E-545.037
CAATTCG502.1827873E-1145.029
TAAGTCG351.2125747E-745.045
CGTTCGC207.034643E-445.026
TCGATAG1650.045.01