FastQCFastQC Report
Thu 26 May 2016
SRR1547562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547562_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1177000
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72290.6141886151231946No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC33570.2852166525063721No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG21630.18377230246389126No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20090.17068819031435856No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCG14170.12039082412914187No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14040.11928632115548003No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC13440.11418861512319456No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT13290.1129141886151232No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC13230.11240441801189464No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12260.10416312659303313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA650.045.0000045
TCGCCTA207.0333557E-445.010
CGGGTCG207.0333557E-445.06
CGGCGTT207.0333557E-445.043
CTATGCG207.0333557E-445.01
GGTACGC207.0333557E-445.08
TGCCCGA207.0333557E-445.024
CGAACCG253.890864E-545.017
GCGACTA351.2119563E-745.033
GTCGATT207.0333557E-445.028
CTCCGGT207.0333557E-445.035
CTCCGAT253.890864E-545.030
CATGCGT453.8562575E-1045.031
GCGCGTA207.0333557E-445.018
CCGGCGT207.0333557E-445.042
ACCGCGA207.0333557E-445.026
ACCGCAT253.890864E-545.033
CGCATGT207.0333557E-445.039
CGCATCA253.890864E-545.035
CCGGATA406.8157533E-945.021