FastQCFastQC Report
Thu 26 May 2016
SRR1547561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547561_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2522055
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291421.155486299862612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC302.1665364E-645.0000048
CTACGCG650.045.0000041
CGCGCGA253.8921367E-545.012
TAAGTCG207.034892E-445.01
TAACGCG406.8212103E-945.01
CCGTCGA207.034892E-445.036
TCGTCGA207.034892E-445.022
CTCGCCG207.034892E-445.030
ATCGGTC207.034892E-445.017
CGTCCGT253.8921367E-545.016
GCGTCCA406.8212103E-945.038
GCGTAAT351.212702E-745.015
GTACGTA351.212702E-745.035
CGACGGT3550.044.366228
CGTTTTT121950.042.952031
CGTTAGG2450.042.24492
GCGCGAC800.042.18759
TCGTAAG750.042.01
TATCGCG603.6379788E-1241.2500041
CGCGTAA501.0822987E-940.531