##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547561_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2522055
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.406597397756986	31.0	31.0	33.0	30.0	34.0
2	31.828423250087727	31.0	31.0	34.0	30.0	34.0
3	31.90313692603849	31.0	31.0	34.0	30.0	34.0
4	35.65773625079548	37.0	35.0	37.0	33.0	37.0
5	35.580622944384636	37.0	35.0	37.0	33.0	37.0
6	35.629879998651894	37.0	35.0	37.0	33.0	37.0
7	36.02033500458951	37.0	35.0	37.0	35.0	37.0
8	35.986310369916595	37.0	35.0	37.0	35.0	37.0
9	37.653346973004155	39.0	38.0	39.0	35.0	39.0
10	37.19615749854781	39.0	37.0	39.0	33.0	39.0
11	36.93247609588213	39.0	37.0	39.0	33.0	39.0
12	36.703244774598495	39.0	35.0	39.0	32.0	39.0
13	36.62580712950352	39.0	35.0	39.0	32.0	39.0
14	37.67583141525462	40.0	36.0	41.0	32.0	41.0
15	37.88305052823987	40.0	36.0	41.0	33.0	41.0
16	37.93895692203382	40.0	36.0	41.0	33.0	41.0
17	37.86750368251287	40.0	36.0	41.0	33.0	41.0
18	37.741875177186856	39.0	36.0	41.0	33.0	41.0
19	37.70010606430074	39.0	36.0	41.0	33.0	41.0
20	37.561353340827225	39.0	35.0	41.0	32.0	41.0
21	37.43589136636592	39.0	35.0	41.0	32.0	41.0
22	37.37477493551885	39.0	35.0	41.0	32.0	41.0
23	37.308635616590436	39.0	35.0	41.0	32.0	41.0
24	37.24597560322832	39.0	35.0	41.0	32.0	41.0
25	37.177800246227775	39.0	35.0	41.0	32.0	41.0
26	37.1907817236341	39.0	35.0	41.0	32.0	41.0
27	37.15075166877804	39.0	35.0	41.0	32.0	41.0
28	37.08775462866591	39.0	35.0	41.0	32.0	41.0
29	37.04675354026776	39.0	35.0	41.0	31.0	41.0
30	36.918052936989874	39.0	35.0	41.0	31.0	41.0
31	36.764839386928514	39.0	35.0	41.0	31.0	41.0
32	36.60894508644736	39.0	35.0	41.0	30.0	41.0
33	36.4213349827819	39.0	35.0	41.0	30.0	41.0
34	36.300584245783696	39.0	35.0	41.0	30.0	41.0
35	36.167164475001535	39.0	35.0	41.0	29.0	41.0
36	36.07477156525135	39.0	35.0	41.0	29.0	41.0
37	36.05428549337742	39.0	35.0	41.0	29.0	41.0
38	35.935885617086065	39.0	35.0	41.0	29.0	41.0
39	35.86188723084945	39.0	35.0	41.0	28.0	41.0
40	35.794234067060394	39.0	35.0	41.0	27.0	41.0
41	35.734501428398666	39.0	35.0	41.0	27.0	41.0
42	35.73339558415657	38.0	35.0	41.0	27.0	41.0
43	35.703847061225865	38.0	35.0	41.0	27.0	41.0
44	35.60410617532131	38.0	35.0	40.0	27.0	41.0
45	35.534467725723665	38.0	35.0	40.0	27.0	41.0
46	35.49881108857658	38.0	34.0	40.0	27.0	41.0
47	35.43466776101235	38.0	34.0	40.0	26.0	41.0
48	35.332268328803295	38.0	34.0	40.0	26.0	41.0
49	35.259296486397005	38.0	34.0	40.0	26.0	41.0
50	35.18503958081802	38.0	34.0	40.0	26.0	41.0
51	34.76351744906435	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	5.0
12	6.0
13	11.0
14	17.0
15	43.0
16	117.0
17	342.0
18	762.0
19	1583.0
20	2765.0
21	4315.0
22	7027.0
23	10633.0
24	16256.0
25	24659.0
26	33939.0
27	39896.0
28	43003.0
29	47713.0
30	55623.0
31	66501.0
32	82661.0
33	105547.0
34	160701.0
35	213485.0
36	188339.0
37	244990.0
38	385426.0
39	785476.0
40	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.170991512873428	24.312237441292915	29.8739321703928	15.642838875440862
2	31.46826694897613	25.842299236138782	30.146170483990236	12.543263330894847
3	28.173929593129408	25.17399501596912	34.11138932338906	12.540686067512405
4	25.668631334368204	26.65401825098977	33.69851172952216	13.978838685119873
5	23.849916040689042	30.947778696340883	31.355144911589957	13.847160351380126
6	22.00384210495013	39.532286171395945	27.340284014424743	11.123587709229179
7	86.87165823108536	2.857392087008412	8.434788297638235	1.836161384267988
8	87.4892101877239	2.3277446368140264	8.047841938419266	2.1352032370428082
9	82.15653504780823	5.77667814540127	9.39301482322947	2.673771983561025
10	36.43719110011479	40.86691210144109	14.121619076507056	8.57427772193707
11	23.87866243995472	24.37583637153036	34.43715541492949	17.30834577358543
12	25.628743227249206	22.955328095541137	32.30448979106324	19.111438886146416
13	24.442686618650267	24.82289244286901	32.68961224081156	18.04480869766916
14	16.374860976465623	30.67006865433149	33.06276032838301	19.89231004081989
15	15.1323424746883	31.887211024343245	36.79368610121508	16.186760399753375
16	21.2882748393671	30.290933385671604	33.72472051561128	14.696071259350013
17	21.833029018003174	29.53781737511672	29.931187067688846	18.697966539191256
18	21.147278707244688	29.726750606152525	32.54901261074798	16.576958075854808
19	21.69040722743953	31.231832771291668	29.84700968059777	17.23075032067104
20	23.06571426872134	31.279294067734448	30.338592933143804	15.316398730400408
21	24.219733510966257	27.535997430666658	33.49784203754478	14.746427020822306
22	21.596475889701058	23.43965536041046	34.9363515070052	20.027517242883285
23	18.546978555186147	27.565893685902964	34.7668865270583	19.120241231852596
24	18.00377073457954	28.543073009906607	35.39272537672652	18.060430878787336
25	19.488948496365065	31.006698902284054	31.569811126244275	17.93454147510661
26	18.912870655080877	33.108675266796325	29.12252111869091	18.855932959431893
27	18.06796441790524	31.965282279728235	32.85507254996421	17.111680752402307
28	17.00050157510443	30.417100340793517	34.27760298645351	18.304795097648544
29	18.656333823013377	28.74084823685447	33.83130026902665	18.771517671105507
30	20.04968170797227	28.676218401264048	33.36663950627564	17.907460384488047
31	21.660352371379687	28.670588072028565	31.06621386131547	18.602845695276272
32	22.753667148416667	29.023554204805208	30.64861789294841	17.574160753829716
33	22.107448092924223	28.323252268487405	31.581032134509357	17.988267504079015
34	19.798616604316717	28.113740580597963	32.486960038540005	19.600682776545316
35	19.575703146838592	28.324600375487453	31.97261756781672	20.12707890985724
36	21.602026918524775	28.562065458524895	32.19707738332431	17.638830239626017
37	20.108879465356626	29.702801881798774	31.812549686664248	18.375768966180356
38	19.962808106881095	30.388988344821982	30.023770298427277	19.62443324986965
39	19.700244443519274	28.77550251679682	31.793953740104797	19.730299299579112
40	20.95560961200291	28.29264230954519	31.864689707401304	18.887058371050593
41	18.662439954719463	28.098118399479787	31.730790962132073	21.508650683668677
42	19.877996316495874	28.857578442976067	31.144641968553422	20.11978327197464
43	20.01137960908862	28.050419201801706	31.595345858833372	20.3428553302763
44	20.651690783904396	29.037709328305688	30.514084744385034	19.796515143404882
45	19.4322090517455	29.843916964538835	29.904026676658518	20.81984730705714
46	20.882018829882774	29.021373443481608	30.216866801080865	19.879740925554756
47	19.45699043042281	28.77811943038514	31.318151269500465	20.44673886969158
48	19.638905575017198	28.702268586529634	31.4863474428591	20.17247839559407
49	20.663784096698922	27.592300723021506	31.766634748250933	19.977280432028643
50	19.16623547067768	28.10866535424485	32.38866717815432	20.336431996923142
51	18.687459234632076	28.875381385417842	31.10717252399333	21.32998685595675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1909.0
1	2950.5
2	3992.0
3	23065.5
4	42139.0
5	28049.0
6	13959.0
7	13807.0
8	13655.0
9	14415.5
10	15176.0
11	16131.0
12	17086.0
13	17197.5
14	17309.0
15	16767.0
16	16225.0
17	15619.5
18	15014.0
19	15286.0
20	15558.0
21	16522.0
22	17486.0
23	18712.0
24	19938.0
25	24520.0
26	37862.5
27	46623.0
28	53260.0
29	59897.0
30	67403.0
31	74909.0
32	83583.5
33	92258.0
34	101084.0
35	109910.0
36	119382.0
37	128854.0
38	135904.0
39	142954.0
40	151188.5
41	159423.0
42	174083.0
43	188743.0
44	192625.5
45	196508.0
46	206419.5
47	216331.0
48	217021.5
49	217712.0
50	201056.5
51	184401.0
52	159371.0
53	134341.0
54	116637.0
55	98933.0
56	88934.5
57	78936.0
58	69748.5
59	60561.0
60	50124.5
61	39688.0
62	32617.0
63	25546.0
64	19907.0
65	14268.0
66	10432.5
67	6597.0
68	5125.5
69	3654.0
70	2420.0
71	1186.0
72	923.0
73	660.0
74	463.5
75	197.5
76	128.0
77	123.0
78	118.0
79	89.0
80	60.0
81	40.5
82	21.0
83	14.0
84	7.0
85	4.5
86	2.0
87	1.0
88	0.0
89	1.5
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2522055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.28645116640035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23755123751815	15.263029090594562
2	9.809490805651224	3.979995123921936
3	3.8025594159768907	2.314213076986531
4	1.9430624171594038	1.576713633558883
5	1.23592230344849	1.2536238727186415
6	0.8055017293168647	0.9804462897742566
7	0.54671671426442	0.7763659348046008
8	0.42536049038613377	0.6903243853067527
9	0.34145764728794403	0.6234267498390749
>10	3.5398899405082207	16.63190655725991
>50	1.1912362184625334	17.520796448481583
>100	1.1145263463666317	35.85481812669417
>500	0.004153511962256522	0.5747335676089901
>1k	0.002373435407003727	0.7903188411892124
>5k	0.0	0.0
>10k+	1.9778628391697726E-4	1.169288301260904
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29142	1.155486299862612	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9650205883694053E-5	0.0	0.0	0.10392318962116211	0.0
2	3.9650205883694053E-5	0.0	0.0	0.3237835812462456	0.0
3	3.9650205883694053E-5	0.0	0.0	0.49550862292852454	0.0
4	3.9650205883694053E-5	0.0	0.0	0.7039101050532205	0.0
5	3.9650205883694053E-5	0.0	0.0	1.2521535018070582	0.0
6	3.9650205883694053E-5	0.0	0.0	1.8117368574436323	0.0
7	3.9650205883694053E-5	0.0	0.0	2.2322669410460914	0.0
8	3.9650205883694053E-5	0.0	0.0	3.042915400338216	0.0
9	3.9650205883694053E-5	0.0	0.0	3.367373035084485	0.0
10	3.9650205883694053E-5	0.0	0.0	3.848131781424275	0.0
11	3.9650205883694053E-5	0.0	0.0	4.512550281417337	0.0
12	3.9650205883694053E-5	0.0	0.0	5.068842669965564	0.0
13	3.9650205883694053E-5	0.0	0.0	5.28057476938449	0.0
14	3.9650205883694053E-5	0.0	0.0	5.3843393581821175	0.0
15	3.9650205883694053E-5	0.0	0.0	5.5142730828629825	0.0
16	3.9650205883694053E-5	0.0	0.0	5.767915449900974	0.0
17	3.9650205883694053E-5	0.0	0.0	6.104069895382932	0.0
18	3.9650205883694053E-5	0.0	0.0	6.449462838835791	0.0
19	3.9650205883694053E-5	0.0	0.0	6.718608436374306	0.0
20	3.9650205883694053E-5	0.0	0.0	6.9909657005894	0.0
21	3.9650205883694053E-5	0.0	0.0	7.295399981364403	0.0
22	3.9650205883694053E-5	0.0	0.0	7.662917739700363	0.0
23	3.9650205883694053E-5	0.0	0.0	8.010055292212105	0.0
24	3.9650205883694053E-5	0.0	0.0	8.25933613660289	0.0
25	7.930041176738811E-5	0.0	0.0	8.484033853345784	0.0
26	7.930041176738811E-5	0.0	0.0	8.687082557676181	0.0
27	7.930041176738811E-5	0.0	0.0	8.915983196242747	0.0
28	7.930041176738811E-5	0.0	0.0	9.150752065280098	0.0
29	7.930041176738811E-5	0.0	0.0	9.419461510553894	0.0
30	1.1895061765108215E-4	0.0	0.0	9.722587334534735	0.0
31	1.1895061765108215E-4	0.0	0.0	9.988798816837857	0.0
32	1.1895061765108215E-4	0.0	0.0	10.222140278463396	0.0
33	1.1895061765108215E-4	0.0	0.0	10.473800135207203	0.0
34	1.1895061765108215E-4	0.0	0.0	10.739694415863255	0.0
35	1.1895061765108215E-4	0.0	0.0	11.031361330343708	0.0
36	1.5860082353477621E-4	0.0	0.0	11.288096413440627	0.0
37	1.9825102941847025E-4	0.0	0.0	11.571555735303155	0.0
38	1.9825102941847025E-4	0.0	0.0	11.885387114872595	0.0
39	1.9825102941847025E-4	0.0	0.0	12.364639153388804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	30	2.1665364E-6	45.000004	8
CTACGCG	65	0.0	45.000004	1
CGCGCGA	25	3.8921367E-5	45.0	12
TAAGTCG	20	7.034892E-4	45.0	1
TAACGCG	40	6.8212103E-9	45.0	1
CCGTCGA	20	7.034892E-4	45.0	36
TCGTCGA	20	7.034892E-4	45.0	22
CTCGCCG	20	7.034892E-4	45.0	30
ATCGGTC	20	7.034892E-4	45.0	17
CGTCCGT	25	3.8921367E-5	45.0	16
GCGTCCA	40	6.8212103E-9	45.0	38
GCGTAAT	35	1.212702E-7	45.0	15
GTACGTA	35	1.212702E-7	45.0	35
CGACGGT	355	0.0	44.3662	28
CGTTTTT	12195	0.0	42.95203	1
CGTTAGG	245	0.0	42.2449	2
GCGCGAC	80	0.0	42.1875	9
TCGTAAG	75	0.0	42.0	1
TATCGCG	60	3.6379788E-12	41.250004	1
CGCGTAA	50	1.0822987E-9	40.5	31
>>END_MODULE