FastQCFastQC Report
Thu 26 May 2016
SRR1547560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547560_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1176727
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64160.5452411646881562No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17170.14591319821844828No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15410.1309564580399702No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC13760.11693451412264698No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT13290.11294038464316701No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13130.11158068099057811No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT12950.11005101438141557No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT11930.10138290359616121No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC11850.10070305176986674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG302.165545E-645.00000444
AACCGTA302.165545E-645.00000435
CTATGCG600.045.0000041
TCTTGCG302.165545E-645.00000430
GTTAGCG302.165545E-645.0000041
TCGGCCC302.165545E-645.00000414
ATCCGCG302.165545E-645.00000415
ATTCCGT600.045.00000439
CCGTAAA302.165545E-645.00000437
CGGATCA302.165545E-645.00000431
TACGACT600.045.00000419
CGCTAGC302.165545E-645.00000424
TCCCGAC302.165545E-645.00000428
ACGTACT302.165545E-645.00000430
ACCCGGT302.165545E-645.00000430
GCTCGCG302.165545E-645.00000422
TCGGTCA302.165545E-645.00000427
TCGCGGC302.165545E-645.00000412
CGCCCGA302.165545E-645.00000437
AATGTCG302.165545E-645.0000041