##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547560_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1176727
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.338963922813022	31.0	31.0	33.0	30.0	34.0
2	31.739366055168276	31.0	31.0	34.0	30.0	34.0
3	31.889667696925454	31.0	31.0	34.0	30.0	34.0
4	35.619478434675166	37.0	35.0	37.0	33.0	37.0
5	35.5038789795764	37.0	35.0	37.0	33.0	37.0
6	35.541711883894905	37.0	35.0	37.0	33.0	37.0
7	35.915360997070685	37.0	35.0	37.0	35.0	37.0
8	35.84629909911135	37.0	35.0	37.0	35.0	37.0
9	37.499941787687376	39.0	37.0	39.0	35.0	39.0
10	37.08175898063017	39.0	37.0	39.0	33.0	39.0
11	36.784637388281226	39.0	35.0	39.0	32.0	39.0
12	36.10928193200292	38.0	35.0	39.0	32.0	39.0
13	35.762850686692836	38.0	35.0	39.0	31.0	39.0
14	36.79520483510619	39.0	35.0	41.0	31.0	41.0
15	37.09317454260844	39.0	35.0	41.0	32.0	41.0
16	37.20640811335169	39.0	35.0	41.0	32.0	41.0
17	37.14450590493802	39.0	35.0	41.0	32.0	41.0
18	37.0822586717225	38.0	35.0	41.0	32.0	41.0
19	37.085775205294006	38.0	35.0	41.0	32.0	41.0
20	36.95915705172058	38.0	35.0	41.0	32.0	41.0
21	36.78122283248366	38.0	35.0	41.0	31.0	41.0
22	36.70352001781212	38.0	35.0	41.0	31.0	41.0
23	36.6267732447713	38.0	35.0	40.0	31.0	41.0
24	36.57381023805861	38.0	35.0	40.0	31.0	41.0
25	36.49071874784891	38.0	35.0	40.0	31.0	41.0
26	36.48071472822498	38.0	35.0	40.0	31.0	41.0
27	36.44092470046154	38.0	35.0	40.0	31.0	41.0
28	36.41838251353118	38.0	35.0	40.0	31.0	41.0
29	36.41737038412478	38.0	35.0	40.0	31.0	41.0
30	36.362287089528834	38.0	35.0	40.0	31.0	41.0
31	36.199905330633186	38.0	35.0	40.0	30.0	41.0
32	36.02917754075499	38.0	35.0	40.0	30.0	41.0
33	35.93752671605224	38.0	35.0	40.0	30.0	41.0
34	35.877460107569554	38.0	35.0	40.0	30.0	41.0
35	35.77569053824719	38.0	35.0	40.0	30.0	41.0
36	35.68488867851252	38.0	35.0	40.0	29.0	41.0
37	35.64296306619972	38.0	35.0	40.0	29.0	41.0
38	35.565686008734396	38.0	34.0	40.0	29.0	41.0
39	35.532020596111074	38.0	35.0	40.0	29.0	41.0
40	35.440956993423285	37.0	34.0	40.0	28.0	41.0
41	35.416563909895835	37.0	34.0	40.0	28.0	41.0
42	35.39731390543431	37.0	34.0	40.0	28.0	41.0
43	35.3451905157271	37.0	34.0	40.0	28.0	41.0
44	35.21106849762094	37.0	34.0	40.0	27.0	41.0
45	35.13199323207507	37.0	34.0	40.0	27.0	41.0
46	35.094490905707104	37.0	34.0	40.0	27.0	41.0
47	35.04238876136946	37.0	34.0	40.0	27.0	41.0
48	34.95453575893134	37.0	34.0	40.0	27.0	41.0
49	34.92180769201352	36.0	34.0	40.0	27.0	41.0
50	34.79556090750021	36.0	34.0	40.0	27.0	41.0
51	34.3693872920397	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	4.0
13	7.0
14	14.0
15	20.0
16	80.0
17	213.0
18	430.0
19	836.0
20	1521.0
21	2402.0
22	3635.0
23	5534.0
24	8123.0
25	11389.0
26	14861.0
27	17975.0
28	20610.0
29	23642.0
30	28652.0
31	35360.0
32	44256.0
33	57966.0
34	101526.0
35	161519.0
36	84145.0
37	101762.0
38	154266.0
39	295862.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.6442292902262	23.700569460886	29.33671106382364	12.318490185064165
2	30.982377390847667	25.302300363635744	29.9721175769741	13.743204668542491
3	29.65930075540036	25.07616464991455	30.7908291387892	14.473705455895889
4	27.034987724425463	27.328853676341243	30.567582795329756	15.068575803903538
5	25.001210986065587	30.46484018808101	29.17456640325241	15.359382422600994
6	22.49765663573624	40.88832838882765	25.810489603790852	10.803525371645248
7	85.76390275739402	4.320203411666427	7.024229069274352	2.8916647616651954
8	85.09586335658143	4.43484342587533	7.185608896540999	3.2836843210022377
9	80.08314587835581	6.8524815016567135	9.208932913071596	3.855439706915878
10	45.65468456149982	30.021491815858738	14.407249939875605	9.916573682765842
11	36.56166638481143	23.754957606989556	24.2191264413921	15.464249566806915
12	37.24168817406247	21.509152080304098	25.309778733724986	15.939381011908454
13	23.21532521986833	34.62306890213278	25.99974335593557	16.161862522063316
14	15.564102803793913	39.25532430206836	27.38919052592487	17.79138236821285
15	14.704345187966283	30.019027352988413	39.48146001578956	15.795167443255743
16	17.82427020030984	25.016677615113785	40.45152359043346	16.707528594142907
17	18.593522541762024	25.869466749721898	27.53085465022898	28.006156058287097
18	21.077531152085403	28.918177283261116	31.065404295133874	18.938887269519608
19	25.358218176348462	29.84880945197994	25.59931063024814	19.19366174142346
20	27.483350003866658	27.5158979100505	26.463997171816402	18.536754914266435
21	23.37908452852701	30.249667085058814	28.02255748359645	18.34869090281773
22	22.53946752305335	24.52871396679094	27.037112261382635	25.89470624877308
23	18.644511428734106	31.266385491282172	27.727501790984654	22.361601288999065
24	19.1669775572414	27.29477610354823	34.99868703616047	18.5395593030499
25	18.426363973971874	28.600856443338174	31.6984313268923	21.274348255797648
26	17.07983245051741	36.63865960413928	25.563278483454532	20.718229461888782
27	17.233054055868525	36.394082909629844	28.870247729507355	17.502615304994276
28	14.909575458028923	31.990682630720634	35.059703737570395	18.040038173680045
29	16.260610999832586	27.797526529092988	34.30328359933952	21.638578871734907
30	19.31102116293754	31.749760139777532	30.51531918618337	18.423899511101556
31	26.64186340587069	29.342489804347142	24.880707249854893	19.134939539927274
32	26.026682484552495	30.47656763208459	25.515093985265914	17.98165589809701
33	24.521320578179985	30.02310646394618	24.99934139354328	20.456231564330555
34	20.294341848194186	29.58791631363944	26.43680309876462	23.680938739401746
35	19.376796827131525	29.09502373957596	29.596754387381267	21.931425045911244
36	26.240835809835243	28.190310921734607	27.58770725920286	17.981146009227288
37	19.151086020801767	31.82785811832311	29.53310326014445	19.487952600730672
38	18.806656089305335	33.905485299478975	25.945525172788592	21.342333438427094
39	19.22578474021587	31.959749372624234	27.98057663332277	20.833889253837125
40	22.96717930327085	28.236965753314063	27.737954512813936	21.05790043060115
41	16.700390149966815	27.02674452103164	29.658365959139203	26.614499369862337
42	21.46887085959615	26.781572956174205	26.43348882111144	25.31606736311821
43	22.516182598002764	26.7248903101569	25.587158278853124	25.17176881298721
44	18.42398449257984	31.225764344661084	28.100400517707165	22.24985064505191
45	18.65751359491199	35.62916462357029	23.819203604574383	21.894118176943337
46	22.16792849998343	33.61153436608491	25.216044163174633	19.004492970757024
47	20.350854531254914	29.314785842425646	27.093794907399932	23.24056471891951
48	20.58897263341455	26.939723487265947	31.82046472971216	20.650839149607343
49	21.42391565758243	27.204695736564215	30.422689374850755	20.948699231002603
50	18.645786150908407	30.72615823381294	29.80223960187877	20.82581601339988
51	17.766482795074815	31.93442489209477	27.138325202022216	23.1607671108082
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1558.0
1	2119.5
2	2681.0
3	6237.0
4	9793.0
5	6844.5
6	3896.0
7	4182.5
8	4469.0
9	5383.0
10	6297.0
11	6800.0
12	7303.0
13	7650.0
14	7997.0
15	7221.0
16	6445.0
17	6268.5
18	6092.0
19	5859.0
20	5626.0
21	5146.5
22	4667.0
23	5169.0
24	5671.0
25	7223.5
26	10843.0
27	12910.0
28	15554.0
29	18198.0
30	21287.0
31	24376.0
32	29034.0
33	33692.0
34	34198.0
35	34704.0
36	34240.0
37	33776.0
38	42190.0
39	50604.0
40	65599.0
41	80594.0
42	92157.0
43	103720.0
44	111708.5
45	119697.0
46	116888.0
47	114079.0
48	112342.0
49	110605.0
50	98565.0
51	86525.0
52	76934.5
53	67344.0
54	59503.0
55	51662.0
56	45884.0
57	40106.0
58	38000.0
59	35894.0
60	33520.0
61	31146.0
62	25658.5
63	20171.0
64	16021.0
65	11871.0
66	9177.5
67	6484.0
68	4823.0
69	3162.0
70	2598.0
71	2034.0
72	1559.0
73	1084.0
74	699.0
75	392.0
76	470.0
77	298.5
78	127.0
79	90.0
80	53.0
81	45.0
82	37.0
83	22.5
84	8.0
85	5.5
86	3.0
87	2.5
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1176727.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.476148028830732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.73367008409801	13.235328291563263
2	10.238379165365554	3.5785485973844975
3	4.099919699022079	2.14952410699287
4	2.151692466442098	1.5041318422424803
5	1.288338815224745	1.1257599923078023
6	0.8378735062816665	0.8785680855128304
7	0.5815510343661889	0.7114290374032203
8	0.4143813567859169	0.5793431945262725
9	0.29305184890043107	0.4609275742355823
>10	2.1345764177561795	7.972795544181717
>50	0.6532506210127572	8.37984284069279
>100	1.5590389726800575	56.76389656619261
>500	0.008860973005443964	0.9839357586260622
>1k	0.004922762780802203	1.1239941144049577
>5k	4.922762780802202E-4	0.5519744537330432
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6416	0.5452411646881562	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1717	0.14591319821844828	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1541	0.1309564580399702	No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	1376	0.11693451412264698	No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT	1329	0.11294038464316701	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1313	0.11158068099057811	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1295	0.11005101438141557	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1193	0.10138290359616121	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	1185	0.10070305176986674	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12347808795073113	0.0
2	0.0	0.0	0.0	0.4326407059581364	0.0
3	0.0	0.0	0.0	0.6095721437512694	0.0
4	0.0	0.0	0.0	0.9379405758514932	0.0
5	0.0	0.0	0.0	1.5830349775266481	0.0
6	0.0	0.0	0.0	2.1137443094277604	0.0
7	0.0	0.0	0.0	2.458004277967617	0.0
8	0.0	0.0	0.0	3.2491818408177937	0.0
9	0.0	0.0	0.0	3.4601058699256497	0.0
10	0.0	0.0	0.0	3.8987802608421496	0.0
11	0.0	0.0	0.0	4.648231917853503	0.0
12	0.0	0.0	0.0	5.183785194016964	0.0
13	0.0	0.0	0.0	5.391904834341355	0.0
14	0.0	0.0	0.0	5.471787423930954	0.0
15	0.0	0.0	0.0	5.604358530058374	0.0
16	0.0	0.0	0.0	5.92439877728649	0.0
17	0.0	0.0	0.0	6.286759800701437	0.0
18	0.0	0.0	0.0	6.694330970564965	0.0
19	0.0	0.0	0.0	6.938737702117823	0.0
20	0.0	0.0	0.0	7.180085100452357	0.0
21	0.0	0.0	0.0	7.495281403418125	0.0
22	0.0	0.0	0.0	7.819740687517156	0.0
23	0.0	0.0	0.0	8.165105415274741	0.0
24	0.0	0.0	0.0	8.413421294828792	0.0
25	0.0	0.0	0.0	8.641936489942017	0.0
26	0.0	0.0	0.0	8.854220222702462	0.0
27	0.0	0.0	0.0	9.061744992678845	0.0
28	0.0	0.0	0.0	9.271309318134113	0.0
29	0.0	0.0	0.0	9.512231809077212	0.0
30	0.0	0.0	0.0	9.802188612991799	0.0
31	0.0	0.0	0.0	10.068095658551218	0.0
32	0.0	0.0	0.0	10.305448927406271	0.0
33	0.0	0.0	0.0	10.534728955824079	0.0
34	0.0	0.0	0.0	10.790438223989081	0.0
35	0.0	0.0	0.0	11.117871859828151	0.0
36	0.0	0.0	0.0	11.37766023895092	0.0
37	0.0	0.0	0.0	11.644502080771495	0.0
38	0.0	0.0	0.0	11.91108897815721	0.0
39	0.0	0.0	0.0	12.205974707812432	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCG	30	2.165545E-6	45.000004	44
AACCGTA	30	2.165545E-6	45.000004	35
CTATGCG	60	0.0	45.000004	1
TCTTGCG	30	2.165545E-6	45.000004	30
GTTAGCG	30	2.165545E-6	45.000004	1
TCGGCCC	30	2.165545E-6	45.000004	14
ATCCGCG	30	2.165545E-6	45.000004	15
ATTCCGT	60	0.0	45.000004	39
CCGTAAA	30	2.165545E-6	45.000004	37
CGGATCA	30	2.165545E-6	45.000004	31
TACGACT	60	0.0	45.000004	19
CGCTAGC	30	2.165545E-6	45.000004	24
TCCCGAC	30	2.165545E-6	45.000004	28
ACGTACT	30	2.165545E-6	45.000004	30
ACCCGGT	30	2.165545E-6	45.000004	30
GCTCGCG	30	2.165545E-6	45.000004	22
TCGGTCA	30	2.165545E-6	45.000004	27
TCGCGGC	30	2.165545E-6	45.000004	12
CGCCCGA	30	2.165545E-6	45.000004	37
AATGTCG	30	2.165545E-6	45.000004	1
>>END_MODULE