FastQCFastQC Report
Thu 26 May 2016
SRR1547559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547559_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1194012
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169101.4162336726934068No Hit
CCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC20690.1732813405560413No Hit
CTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCT20600.17252757928731033Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC17360.1453921736129955No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15260.12780441067593962No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCC12750.10678284640355373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACG351.2119744E-745.00000426
CTAGACG700.045.0000041
AGCCGTA253.8908962E-545.00000431
CCTTACG253.8908962E-545.0000041
TCCGCTG502.1827873E-1145.00000434
TCACCGA253.8908962E-545.00000414
CTCGTCC351.2119744E-745.00000437
TTAGCCG253.8908962E-545.00000429
GCGCGCG351.2119744E-745.0000041
CTTTTCG253.8908962E-545.00000432
TTTCGCA253.8908962E-545.00000421
CGCATAC351.2119744E-745.00000442
CACGCTT253.8908962E-545.00000428
GGTCCGA253.8908962E-545.0000048
CGCGCGT253.8908962E-545.00000439
CGCGCAT253.8908962E-545.00000412
GCGCATT253.8908962E-545.00000413
GCGCATC253.8908962E-545.00000444
CCTAACG253.8908962E-545.00000441
CCTCGCG253.8908962E-545.0000041