##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547559_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1194012
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30140651852745	31.0	31.0	33.0	30.0	34.0
2	31.69869900805017	31.0	31.0	34.0	30.0	34.0
3	31.783209883987766	31.0	31.0	34.0	30.0	34.0
4	35.53747533525626	37.0	35.0	37.0	33.0	37.0
5	35.435652238000955	37.0	35.0	37.0	33.0	37.0
6	35.484696133707196	37.0	35.0	37.0	33.0	37.0
7	35.92280730846926	37.0	35.0	37.0	35.0	37.0
8	35.884429972228084	37.0	35.0	37.0	35.0	37.0
9	37.55893659360208	39.0	37.0	39.0	35.0	39.0
10	37.040569106508144	39.0	37.0	39.0	33.0	39.0
11	36.71878758337437	39.0	35.0	39.0	32.0	39.0
12	36.10534148735523	37.0	35.0	39.0	32.0	39.0
13	35.843938754384375	38.0	35.0	39.0	31.0	39.0
14	36.84711962693842	39.0	35.0	41.0	31.0	41.0
15	37.12280948600181	39.0	35.0	41.0	32.0	41.0
16	37.25161974921525	39.0	35.0	41.0	32.0	41.0
17	37.213606730920624	39.0	35.0	41.0	32.0	41.0
18	37.168810698719945	39.0	35.0	41.0	32.0	41.0
19	37.11989494242939	38.0	35.0	41.0	32.0	41.0
20	36.95135727279123	38.0	35.0	41.0	32.0	41.0
21	36.79864021467121	38.0	35.0	41.0	32.0	41.0
22	36.71131780920125	38.0	35.0	41.0	31.0	41.0
23	36.64458397403041	38.0	35.0	40.0	31.0	41.0
24	36.52263126333739	38.0	35.0	40.0	31.0	41.0
25	36.437900121606816	38.0	35.0	40.0	31.0	41.0
26	36.3898068026117	38.0	35.0	40.0	31.0	41.0
27	36.32523877481968	38.0	35.0	40.0	31.0	41.0
28	36.284354763603716	38.0	35.0	40.0	31.0	41.0
29	36.25280315440716	37.0	35.0	40.0	31.0	41.0
30	36.13385543863881	37.0	35.0	40.0	31.0	41.0
31	35.99129405734616	37.0	35.0	40.0	30.0	41.0
32	35.78817884577374	37.0	35.0	40.0	30.0	41.0
33	35.657443978787484	37.0	35.0	40.0	30.0	41.0
34	35.61035483730482	37.0	35.0	40.0	30.0	41.0
35	35.488815020284555	37.0	35.0	40.0	29.0	41.0
36	35.37016378394857	37.0	35.0	40.0	29.0	41.0
37	35.28653145864531	37.0	34.0	40.0	28.0	41.0
38	35.197505552708016	37.0	34.0	40.0	27.0	41.0
39	35.18362462018807	37.0	34.0	40.0	27.0	41.0
40	35.07783925119681	36.0	34.0	40.0	27.0	41.0
41	35.03529864021467	36.0	34.0	40.0	27.0	41.0
42	34.991759714307726	36.0	34.0	40.0	27.0	41.0
43	34.95140082344231	36.0	34.0	40.0	27.0	41.0
44	34.835984060461705	36.0	34.0	40.0	26.0	41.0
45	34.775199076726196	36.0	34.0	40.0	26.0	41.0
46	34.71511006589549	36.0	34.0	40.0	26.0	41.0
47	34.62684880888969	35.0	34.0	40.0	26.0	41.0
48	34.538417536842175	35.0	34.0	40.0	25.0	41.0
49	34.51652077198554	36.0	34.0	40.0	26.0	41.0
50	34.3493038595927	35.0	34.0	40.0	25.0	41.0
51	33.9313641738944	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	3.0
11	1.0
12	4.0
13	5.0
14	12.0
15	29.0
16	73.0
17	182.0
18	410.0
19	801.0
20	1469.0
21	2489.0
22	3903.0
23	5845.0
24	8737.0
25	12462.0
26	15995.0
27	18631.0
28	21535.0
29	25163.0
30	30126.0
31	36943.0
32	46582.0
33	60604.0
34	102782.0
35	170375.0
36	109168.0
37	108432.0
38	144356.0
39	266798.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.26241612312104	25.61197039895746	29.545096699195657	13.580516778725842
2	30.332274717507023	26.503251223605794	29.86176018331474	13.302713875572438
3	28.51755258741118	24.566587270479694	33.575793208108465	13.340066934000664
4	26.047644412283965	27.404833452260114	32.91030575907109	13.637216376384828
5	23.651521090240298	31.099184932814744	30.849019942848145	14.40027403409681
6	20.223079835043535	41.32186276184829	27.356006472296762	11.099050930811416
7	85.25500581233689	4.2015490631584935	8.358877465218105	2.184567659286506
8	85.19336489080511	3.5599307209642785	8.036351393453332	3.210352994777272
9	81.32338703463617	5.24383339530926	10.041858875790192	3.3909206942643793
10	47.3703781871539	24.71616700669675	16.294894858678138	11.618559947471214
11	36.36261612111101	25.41121864771878	22.512671564439888	15.713493666730319
12	32.89514678244439	25.168088762927006	25.637263277085992	16.299501177542606
13	21.691323035279378	37.15046414943903	26.778709091700918	14.379503723580667
14	15.208306114176406	41.57872785198139	27.03641169435483	16.176554339487375
15	14.150611551642697	31.9372837123915	39.848008227723	14.064096508242798
16	16.648995152477532	28.63781938539981	38.89667775533244	15.816507706790217
17	16.99974539619367	30.09567743037758	25.778300385590764	27.126276787837977
18	19.28129700539023	32.48074558714652	30.969202989584694	17.268754417878547
19	23.761067727962534	34.47561666046907	24.25344133894802	17.509874272620376
20	25.510128876426702	32.65997326660033	24.38836460605086	17.4415332509221
21	20.674666586265463	35.11045115124471	27.07627729034549	17.138604972144332
22	20.231371208999573	30.23051694622835	25.70987561264041	23.82823623213167
23	17.573273970445857	34.996633199666334	25.841951337172492	21.588141492715316
24	17.50803174507459	32.818765640546324	32.395403061275765	17.277799553103318
25	16.995641584841696	32.834510875937596	30.805469291765913	19.3643782474548
26	15.313665189294582	40.884597474732246	25.63374572449858	18.16799161147459
27	17.82821278178109	39.60554835294788	26.975440782839705	15.59079808243133
28	14.92129057329407	35.11983129147781	33.91481827653323	16.044059858694887
29	15.324469100813056	32.12153646697018	33.33827465720612	19.215719775010637
30	19.459017162306576	37.61402732970858	27.412371064947422	15.514584443037425
31	26.199401681055136	33.713480266529984	22.27515301353755	17.811965038877332
32	25.091205113516445	35.406260573595574	23.11065550429979	16.39187880858819
33	24.257210145291673	34.79797522972968	23.3286600134672	17.61615461151144
34	17.977290010485657	35.08021694924339	24.460725687849035	22.48176735242192
35	19.44888326080475	33.813060505254555	27.40943977112458	19.32861646281612
36	26.851991437271987	34.40610312124166	23.524051684572683	15.217853756913666
37	19.70532959467744	36.455663762173245	26.299986934804675	17.53901970834464
38	18.255595421151547	38.600700830477415	23.87346190825553	19.27024184011551
39	18.897297514597845	37.807408970764115	26.001999979899697	17.293293534738343
40	22.834527626188013	33.35200986254744	24.067597310579796	19.74586520068475
41	18.920831616432665	31.15739205301119	25.6599598664	24.261816464156137
42	20.99652264801359	32.64858309631729	23.638958402428116	22.715935853241007
43	20.999621444340594	31.495412106410992	25.72964090813158	21.77532554111684
44	18.336164125653678	35.6843984817573	26.218413215277568	19.76102417731145
45	18.894868728287488	38.1677906084696	22.872634445884966	20.06470621735795
46	22.52799804357075	36.23263417788095	23.23309983484253	18.00626794370576
47	20.08522527411785	32.97353795439242	25.59044632717259	21.35079044431714
48	20.013617953588405	31.677403577183476	28.681202533977885	19.627775935250234
49	20.761767888429933	29.71938305477667	29.402300814397176	20.116548242396224
50	18.876359701577538	33.98717935833141	27.615802856252703	19.520658083838356
51	16.805861247625653	35.44796869713202	25.322274818008527	22.423895237233797
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1894.0
1	2202.5
2	2511.0
3	11089.5
4	19668.0
5	12583.5
6	5499.0
7	6450.5
8	7402.0
9	10446.0
10	13490.0
11	16424.5
12	19359.0
13	19680.0
14	20001.0
15	18264.5
16	16528.0
17	14542.5
18	12557.0
19	10630.0
20	8703.0
21	7618.5
22	6534.0
23	7049.5
24	7565.0
25	7446.5
26	9066.5
27	10805.0
28	12547.5
29	14290.0
30	17153.5
31	20017.0
32	21589.5
33	23162.0
34	25776.0
35	28390.0
36	31721.0
37	35052.0
38	40839.0
39	46626.0
40	59575.0
41	72524.0
42	87075.5
43	101627.0
44	107905.5
45	114184.0
46	120742.0
47	127300.0
48	129788.0
49	132276.0
50	118884.0
51	105492.0
52	86692.0
53	67892.0
54	59357.5
55	50823.0
56	43308.5
57	35794.0
58	29358.5
59	22923.0
60	19855.0
61	16787.0
62	12756.5
63	8726.0
64	6673.5
65	4621.0
66	3909.5
67	3198.0
68	2057.0
69	916.0
70	860.5
71	805.0
72	569.0
73	333.0
74	232.0
75	171.0
76	211.0
77	122.0
78	33.0
79	21.5
80	10.0
81	9.0
82	8.0
83	8.5
84	9.0
85	5.5
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1194012.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.554217366600888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.02124615072746	16.243828406969747
2	11.198728033552129	5.051570925963633
3	5.029866796890786	3.4033412718637
4	2.7326470914314025	2.465308659454142
5	1.7967631995330875	2.026229387928927
6	1.126593624688463	1.5245662497030223
7	0.7808487110528107	1.2328002091660655
8	0.5365798306003824	0.9681710507115925
9	0.4152490588938339	0.8429055782011184
>10	2.882412661813765	13.551784883104926
>50	0.5939551779851593	9.688812235685486
>100	0.8738391662751102	39.41633074550024
>500	0.007889347588898237	1.1420234181335036
>1k	0.003005465748151709	1.0095019293398546
>5k	0.0	0.0
>10k+	3.7568321851896364E-4	1.4328250482740426
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16910	1.4162336726934068	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	2069	0.1732813405560413	No Hit
CTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCT	2060	0.17252757928731033	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	1736	0.1453921736129955	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1526	0.12780441067593962	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCC	1275	0.10678284640355373	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.375125208121862E-5	0.0	0.0	0.1867652921411175	0.0
2	8.375125208121862E-5	0.0	0.0	0.6814001869327947	0.0
3	8.375125208121862E-5	0.0	0.0	0.9875947645417299	0.0
4	8.375125208121862E-5	0.0	0.0	1.4056810149311731	0.0
5	8.375125208121862E-5	0.0	0.0	2.523090220198792	0.0
6	8.375125208121862E-5	0.0	0.0	3.5867311216302684	0.0
7	8.375125208121862E-5	0.0	0.0	4.408247153294941	0.0
8	8.375125208121862E-5	0.0	0.0	6.0558855354887555	0.0
9	8.375125208121862E-5	0.0	0.0	6.5905535287752555	0.0
10	8.375125208121862E-5	0.0	0.0	7.48225310968399	0.0
11	8.375125208121862E-5	0.0	0.0	8.916242047818615	0.0
12	8.375125208121862E-5	0.0	0.0	9.877287665450599	0.0
13	8.375125208121862E-5	0.0	0.0	10.21689899263994	0.0
14	8.375125208121862E-5	0.0	0.0	10.341772109493037	0.0
15	8.375125208121862E-5	0.0	0.0	10.530044924171616	0.0
16	8.375125208121862E-5	0.0	0.0	10.990509308114156	0.0
17	8.375125208121862E-5	0.0	0.0	11.550051423268778	0.0
18	8.375125208121862E-5	0.0	0.0	12.107332254617207	0.0
19	8.375125208121862E-5	0.0	0.0	12.461767553424924	0.0
20	8.375125208121862E-5	0.0	0.0	12.787811177777108	0.0
21	8.375125208121862E-5	0.0	0.0	13.227672753707667	0.0
22	8.375125208121862E-5	0.0	0.0	13.683195813777417	0.0
23	8.375125208121862E-5	0.0	0.0	14.172973135948382	0.0
24	8.375125208121862E-5	0.0	0.0	14.519870822068789	0.0
25	1.6750250416243723E-4	0.0	0.0	14.807640124219857	0.0
26	1.6750250416243723E-4	0.0	0.0	15.076732897156813	0.0
27	1.6750250416243723E-4	0.0	0.0	15.328405409660874	0.0
28	1.6750250416243723E-4	0.0	0.0	15.593561873750012	0.0
29	1.6750250416243723E-4	0.0	0.0	15.890292559873771	0.0
30	1.6750250416243723E-4	0.0	0.0	16.235766474708797	0.0
31	1.6750250416243723E-4	0.0	0.0	16.560721332783924	0.0
32	1.6750250416243723E-4	0.0	0.0	16.84438682358301	0.0
33	1.6750250416243723E-4	0.0	0.0	17.13542242456525	0.0
34	1.6750250416243723E-4	0.0	0.0	17.441198245913775	0.0
35	1.6750250416243723E-4	0.0	0.0	17.795884798477736	0.0
36	1.6750250416243723E-4	0.0	0.0	18.097389305970125	0.0
37	1.6750250416243723E-4	0.0	0.0	18.398307554697944	0.0
38	1.6750250416243723E-4	0.0	0.0	18.711872242490024	0.0
39	1.6750250416243723E-4	0.0	0.0	19.098216768340688	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACG	35	1.2119744E-7	45.000004	26
CTAGACG	70	0.0	45.000004	1
AGCCGTA	25	3.8908962E-5	45.000004	31
CCTTACG	25	3.8908962E-5	45.000004	1
TCCGCTG	50	2.1827873E-11	45.000004	34
TCACCGA	25	3.8908962E-5	45.000004	14
CTCGTCC	35	1.2119744E-7	45.000004	37
TTAGCCG	25	3.8908962E-5	45.000004	29
GCGCGCG	35	1.2119744E-7	45.000004	1
CTTTTCG	25	3.8908962E-5	45.000004	32
TTTCGCA	25	3.8908962E-5	45.000004	21
CGCATAC	35	1.2119744E-7	45.000004	42
CACGCTT	25	3.8908962E-5	45.000004	28
GGTCCGA	25	3.8908962E-5	45.000004	8
CGCGCGT	25	3.8908962E-5	45.000004	39
CGCGCAT	25	3.8908962E-5	45.000004	12
GCGCATT	25	3.8908962E-5	45.000004	13
GCGCATC	25	3.8908962E-5	45.000004	44
CCTAACG	25	3.8908962E-5	45.000004	41
CCTCGCG	25	3.8908962E-5	45.000004	1
>>END_MODULE