FastQCFastQC Report
Thu 26 May 2016
SRR1547558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547558_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1211052
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80580.6653719245746673No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT14600.12055634274993972Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC14470.11948289586244025Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC14360.1185745946499407Illumina Single End Adapter 1 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13210.10907871833744547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCA351.2119926E-745.00000439
TCGCACG351.2119926E-745.0000041
ACTGCGC453.8562575E-1045.00000433
GTCGATG650.045.0000041
ACTTGCG351.2119926E-745.0000041
TAGAACG700.045.0000041
TATGCGA351.2119926E-745.00000439
TTATCGG453.8562575E-1045.0000041
TCAGGCG351.2119926E-745.00000413
GTGGACG750.045.0000041
TGCGCAA453.8562575E-1045.0000041
GTCACGT351.2119926E-745.00000433
CGGGCTT900.045.0000046
CAAATCG351.2119926E-745.0000041
ATAACGC351.2119926E-745.00000411
GCTAACG900.045.0000041
CGATCCG351.2119926E-745.00000416
TGCACGT453.8562575E-1045.00000440
AGTCGGC351.2119926E-745.00000430
AGTCGAT453.8562575E-1045.00000410