##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547558_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1211052
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.422400524502663	31.0	31.0	33.0	30.0	34.0
2	31.841662455451953	31.0	31.0	34.0	30.0	34.0
3	31.96891710677989	33.0	31.0	34.0	30.0	34.0
4	35.708590547722146	37.0	35.0	37.0	33.0	37.0
5	35.61178793313582	37.0	35.0	37.0	33.0	37.0
6	35.653955404061925	37.0	35.0	37.0	33.0	37.0
7	36.00088683227475	37.0	35.0	37.0	35.0	37.0
8	35.95932792316102	37.0	35.0	37.0	35.0	37.0
9	37.631911759362936	39.0	37.0	39.0	35.0	39.0
10	37.16188157073354	39.0	37.0	39.0	33.0	39.0
11	36.92503872666079	39.0	37.0	39.0	33.0	39.0
12	36.37438937386669	38.0	35.0	39.0	32.0	39.0
13	36.13655152710206	38.0	35.0	39.0	32.0	39.0
14	37.18251817428153	39.0	35.0	41.0	32.0	41.0
15	37.41710265124867	39.0	35.0	41.0	32.0	41.0
16	37.53203082939461	39.0	35.0	41.0	33.0	41.0
17	37.486735499384004	39.0	35.0	41.0	33.0	41.0
18	37.43975320630328	39.0	35.0	41.0	33.0	41.0
19	37.44273408573703	39.0	36.0	41.0	33.0	41.0
20	37.31902676350809	39.0	35.0	41.0	32.0	41.0
21	37.16189891102942	39.0	35.0	41.0	32.0	41.0
22	37.06877326489697	39.0	35.0	41.0	32.0	41.0
23	36.999841460152	39.0	35.0	41.0	32.0	41.0
24	36.92864798538791	38.0	35.0	41.0	32.0	41.0
25	36.88042792547306	38.0	35.0	41.0	32.0	41.0
26	36.88983379739268	38.0	35.0	41.0	32.0	41.0
27	36.847999095001704	38.0	35.0	41.0	32.0	41.0
28	36.825240369529965	38.0	35.0	41.0	32.0	41.0
29	36.77941079326074	38.0	35.0	41.0	32.0	41.0
30	36.73416748413776	38.0	35.0	40.0	31.0	41.0
31	36.5905972658482	38.0	35.0	40.0	31.0	41.0
32	36.43620752866103	38.0	35.0	41.0	31.0	41.0
33	36.334604955030834	38.0	35.0	41.0	30.0	41.0
34	36.2973001985051	38.0	35.0	41.0	30.0	41.0
35	36.20413822032415	38.0	35.0	41.0	30.0	41.0
36	36.13029250601956	38.0	35.0	41.0	30.0	41.0
37	36.06537208972034	38.0	35.0	40.0	30.0	41.0
38	35.963246830028766	38.0	35.0	40.0	30.0	41.0
39	35.954272814049276	38.0	35.0	40.0	30.0	41.0
40	35.8991075527723	38.0	35.0	40.0	30.0	41.0
41	35.87348107265419	38.0	35.0	40.0	30.0	41.0
42	35.845611088541204	38.0	35.0	40.0	30.0	41.0
43	35.81139785905147	38.0	35.0	40.0	30.0	41.0
44	35.70004508476927	38.0	35.0	40.0	29.0	41.0
45	35.63112484022156	38.0	35.0	40.0	29.0	41.0
46	35.60864355948382	38.0	35.0	40.0	29.0	41.0
47	35.530657643106984	38.0	35.0	40.0	29.0	41.0
48	35.47103262287664	37.0	34.0	40.0	29.0	41.0
49	35.43493508123516	37.0	34.0	40.0	28.0	41.0
50	35.31870142652834	37.0	34.0	40.0	28.0	41.0
51	34.857321568355445	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	4.0
11	1.0
12	5.0
13	4.0
14	9.0
15	17.0
16	42.0
17	106.0
18	293.0
19	595.0
20	1065.0
21	1754.0
22	2852.0
23	4429.0
24	6681.0
25	9574.0
26	12982.0
27	15292.0
28	17668.0
29	21432.0
30	25572.0
31	32587.0
32	41847.0
33	55687.0
34	96134.0
35	157427.0
36	94318.0
37	110824.0
38	165085.0
39	336643.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.95077337719602	25.080591089399963	30.140984862747427	11.827650670656586
2	30.88496612862206	26.47582432463676	29.433500791047784	13.205708755693395
3	27.824073615336086	26.304403113986847	32.222150659096386	13.649372611580675
4	27.04590719473648	27.85272638994857	31.41805636752179	13.68331004779316
5	24.2050712933879	32.408600126171294	29.51912882353524	13.867199756905565
6	21.48231454966426	41.49342885359175	26.50282564249925	10.52143095424474
7	86.43906289738177	4.674200612360163	6.882528578459059	2.004207911798998
8	87.01385241921899	4.072574918335463	6.334822947321833	2.5787497151237107
9	81.95684413220901	6.801937489059099	7.895201857558552	3.346016521173327
10	43.04290814927848	28.35146632844833	16.505814779216745	12.09981074305645
11	34.78603726347011	26.368562208724317	22.122666904476436	16.722733623329137
12	32.76465420147112	23.91722238186304	25.961312974174522	17.356810442491323
13	24.30192923177535	33.67485458923316	26.947893236624026	15.07532294236746
14	18.271387190640866	38.66481373219317	26.63428159979918	16.429517477366783
15	15.742016032342129	32.06955605539646	37.442322873006276	14.746105039255127
16	18.212512757503394	28.35584268883582	37.32655575483134	16.10508879882945
17	18.565263919303217	27.87336959932356	27.558519369936217	26.002847111437
18	20.42207931616479	30.027942648209986	30.924270799272037	18.625707236353186
19	23.95380214887552	32.44906081654628	25.950578505299525	17.64655852927868
20	27.188097620911407	30.16253637333492	25.81573706166209	16.833628944091583
21	23.734571265313132	31.809947054296593	27.443743125811277	17.011738554578994
22	22.51653933935124	27.507654502036242	27.212704326486392	22.763101832126118
23	18.55717178122822	32.008699874159	27.18578558146141	22.248342763151378
24	18.529014443640733	30.701076419509647	33.11269871153345	17.657210425316173
25	17.9973279429785	31.97055122323402	30.675396267047162	19.356724566740322
26	17.252355803053874	36.35244399084432	26.8009135858741	19.594286620227702
27	17.765380842441118	37.17041051911891	28.52420870449824	16.53999993394173
28	17.10719275472895	33.09279865769595	33.129460997545934	16.670547590029162
29	17.23683210960388	29.77865525179761	32.92765298269604	20.05685965590247
30	18.382695375590806	33.8001175837206	29.883275036910057	17.93391200377853
31	24.74286818402513	32.01522312832149	25.673216344137163	17.568692343516215
32	24.562281388412718	31.340437900271827	26.571691388974216	17.525589322341236
33	23.76305889425062	32.68265937383366	24.553446094800226	19.0008356371155
34	20.190379934139905	32.201755168233895	25.991452059862002	21.616412837764194
35	19.309327758015346	31.841407305384077	28.410093043073292	20.43917189352728
36	25.257874971512372	30.57936405703471	26.606784844911697	17.55597612654122
37	20.287733309552355	35.09163933505746	27.968906372311015	16.651720983079173
38	20.272127043264867	34.7953679941076	24.34610569983783	20.586399262789705
39	19.393882343615303	33.52011309175824	27.311213721623844	19.774790843002613
40	22.156521767851423	32.116209708583945	25.97551550222451	19.751753021340125
41	18.568236541453214	30.03149328022248	27.66099226127367	23.73927791705063
42	20.570710423664714	30.57779517312221	26.190700316749403	22.66079408646367
43	22.084022816526456	29.17752499479791	26.31654132109934	22.42191086757629
44	19.559771174152722	31.930503397046532	27.467689248686266	21.04203618011448
45	18.905216291290547	36.034043129444484	25.110069592387447	19.950670986877526
46	22.84088544505108	33.38320732718331	25.736136846312128	18.03977038145348
47	20.052813586864975	31.38618325224681	26.453612231349272	22.107390929538948
48	20.54288337742723	30.02802522104749	30.006638856134998	19.42245254539029
49	21.177538206451914	28.969936881323015	29.220710588810388	20.631814323414684
50	20.081796652827457	31.841737596734077	28.36583400217332	19.710631748265143
51	19.07828895869046	32.615775375458696	26.027784108361985	22.27815155748886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1373.0
1	1832.0
2	2291.0
3	6452.5
4	10614.0
5	7090.0
6	3566.0
7	4452.5
8	5339.0
9	6444.5
10	7550.0
11	8798.0
12	10046.0
13	10130.5
14	10215.0
15	9797.0
16	9379.0
17	8739.5
18	8100.0
19	6941.5
20	5783.0
21	5626.5
22	5470.0
23	5579.0
24	5688.0
25	7017.0
26	10430.0
27	12514.0
28	15473.5
29	18433.0
30	21924.5
31	25416.0
32	31163.0
33	36910.0
34	39690.0
35	42470.0
36	45944.5
37	49419.0
38	54025.5
39	58632.0
40	70245.5
41	81859.0
42	96988.0
43	112117.0
44	113471.0
45	114825.0
46	119092.0
47	123359.0
48	120127.5
49	116896.0
50	107980.5
51	99065.0
52	86156.0
53	73247.0
54	60117.5
55	46988.0
56	41368.0
57	35748.0
58	31790.5
59	27833.0
60	23257.0
61	18681.0
62	15023.0
63	11365.0
64	8514.0
65	5663.0
66	4444.0
67	3225.0
68	2184.0
69	1143.0
70	819.0
71	495.0
72	487.0
73	479.0
74	378.0
75	184.0
76	91.0
77	95.5
78	100.0
79	56.0
80	12.0
81	7.0
82	2.0
83	10.0
84	18.0
85	11.5
86	5.0
87	3.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1211052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.624296374348052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.71332401285409	14.465721171665958
2	10.86806308547648	4.26556182009004
3	4.322674223170005	2.5448832025572874
4	2.3245143037757225	1.8246783049482438
5	1.435518247899821	1.4085517773785463
6	0.9152042197277985	1.0776143310595363
7	0.6649948601398689	0.9135039355962044
8	0.46259417206649683	0.7262468106943274
9	0.34618920771794026	0.6114347652471841
>10	2.878563040070734	12.357604384625647
>50	0.8213860104715621	11.702232383183556
>100	1.2388884331087096	46.12257227705507
>500	0.00468147466994016	0.5578218611377815
>1k	0.0029791202445073744	0.7485787305551634
>5k	4.2558860635819637E-4	0.6729942442054565
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8058	0.6653719245746673	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	1460	0.12055634274993972	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	1447	0.11948289586244025	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	1436	0.1185745946499407	Illumina Single End Adapter 1 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1321	0.10907871833744547	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1289787721749355	0.0
2	0.0	0.0	0.0	0.4829685265372585	0.0
3	0.0	0.0	0.0	0.6770972674996615	0.0
4	0.0	0.0	0.0	0.9723777343995138	0.0
5	0.0	0.0	0.0	1.7062025412616468	0.0
6	0.0	0.0	0.0	2.262330601823869	0.0
7	0.0	0.0	0.0	2.6732956140611632	0.0
8	0.0	0.0	0.0	3.5842391573607078	0.0
9	0.0	0.0	0.0	3.835756020385582	0.0
10	0.0	0.0	0.0	4.426729818372786	0.0
11	0.0	0.0	0.0	5.407364836522296	0.0
12	0.0	0.0	0.0	6.096930602484451	0.0
13	0.0	0.0	0.0	6.350429213609325	0.0
14	0.0	0.0	0.0	6.435974673259282	0.0
15	0.0	0.0	0.0	6.582954324009209	0.0
16	0.0	0.0	0.0	6.957422142071521	0.0
17	0.0	0.0	0.0	7.422802654221289	0.0
18	0.0	0.0	0.0	7.886614282458557	0.0
19	0.0	0.0	0.0	8.18016071977091	0.0
20	0.0	0.0	0.0	8.469578515208266	0.0
21	0.0	0.0	0.0	8.849991577570576	0.0
22	0.0	0.0	0.0	9.246341197570377	0.0
23	0.0	0.0	0.0	9.678362283370161	0.0
24	0.0	0.0	0.0	9.986111248732508	0.0
25	0.0	0.0	0.0	10.249436027519875	0.0
26	0.0	0.0	0.0	10.485264051419758	0.0
27	0.0	0.0	0.0	10.71811945316964	0.0
28	0.0	0.0	0.0	10.964929664457017	0.0
29	0.0	0.0	0.0	11.25773294623187	0.0
30	0.0	0.0	0.0	11.566720504156716	0.0
31	0.0	0.0	0.0	11.865386457394067	0.0
32	0.0	0.0	0.0	12.14175774450643	0.0
33	0.0	0.0	0.0	12.425643159831287	0.0
34	0.0	0.0	0.0	12.714070081218644	0.0
35	0.0	0.0	0.0	13.051462695243474	0.0
36	0.0	0.0	0.0	13.348064327543327	0.0
37	0.0	0.0	0.0	13.664400868005668	0.0
38	0.0	0.0	0.0	13.969507502568016	0.0
39	0.0	0.0	0.0	14.316891429930342	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCA	35	1.2119926E-7	45.000004	39
TCGCACG	35	1.2119926E-7	45.000004	1
ACTGCGC	45	3.8562575E-10	45.000004	33
GTCGATG	65	0.0	45.000004	1
ACTTGCG	35	1.2119926E-7	45.000004	1
TAGAACG	70	0.0	45.000004	1
TATGCGA	35	1.2119926E-7	45.000004	39
TTATCGG	45	3.8562575E-10	45.000004	1
TCAGGCG	35	1.2119926E-7	45.000004	13
GTGGACG	75	0.0	45.000004	1
TGCGCAA	45	3.8562575E-10	45.000004	1
GTCACGT	35	1.2119926E-7	45.000004	33
CGGGCTT	90	0.0	45.000004	6
CAAATCG	35	1.2119926E-7	45.000004	1
ATAACGC	35	1.2119926E-7	45.000004	11
GCTAACG	90	0.0	45.000004	1
CGATCCG	35	1.2119926E-7	45.000004	16
TGCACGT	45	3.8562575E-10	45.000004	40
AGTCGGC	35	1.2119926E-7	45.000004	30
AGTCGAT	45	3.8562575E-10	45.000004	10
>>END_MODULE