FastQCFastQC Report
Thu 26 May 2016
SRR1547557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547557_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2812206
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81570.29005698729040474No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG1050.045.0000042
CTATACG302.1666274E-645.0000041
TAAGCGT302.1666274E-645.00000437
TACCGAA600.045.00000416
GTACCGT253.892251E-545.039
CGAACCG406.8212103E-945.05
CGCATTA502.1827873E-1145.037
TAGCGAT207.035031E-445.01
CGTACAC207.035031E-445.030
CCGCGAC207.035031E-445.012
TATCGCG453.8562575E-1045.01
ACGACGT253.892251E-545.015
CTTACGT207.035031E-445.035
TTAGTCG551.8189894E-1244.9999961
GCGTAAG1150.043.0434761
CGATAGA900.042.510
CCGTCGC750.042.024
GGACGTA650.041.538468
CGGTCTA8250.040.90909231
CACGCGA556.184564E-1140.9090942