##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547557_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2812206
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.378592820013896	31.0	31.0	33.0	30.0	34.0
2	31.79706607552932	31.0	31.0	34.0	30.0	34.0
3	31.966200200127588	33.0	31.0	34.0	30.0	34.0
4	35.69554861912676	37.0	35.0	37.0	33.0	37.0
5	35.59416664355314	37.0	35.0	37.0	33.0	37.0
6	35.63598470382326	37.0	35.0	37.0	33.0	37.0
7	35.95840454077688	37.0	35.0	37.0	35.0	37.0
8	35.89088921650832	37.0	35.0	37.0	35.0	37.0
9	37.51765553448076	39.0	37.0	39.0	35.0	39.0
10	37.041640619499425	39.0	37.0	39.0	33.0	39.0
11	36.87479544528388	39.0	37.0	39.0	33.0	39.0
12	36.53320347086949	39.0	35.0	39.0	32.0	39.0
13	36.39434735577692	39.0	35.0	39.0	32.0	39.0
14	37.42217319783828	40.0	36.0	41.0	32.0	41.0
15	37.63196472804624	40.0	36.0	41.0	32.0	41.0
16	37.69804879158924	40.0	36.0	41.0	33.0	41.0
17	37.626775918976065	40.0	36.0	41.0	32.0	41.0
18	37.562675707256155	39.0	36.0	41.0	32.0	41.0
19	37.572776318662285	39.0	36.0	41.0	32.0	41.0
20	37.46408762373738	39.0	35.0	41.0	32.0	41.0
21	37.33067065499469	39.0	35.0	41.0	32.0	41.0
22	37.288560297503096	39.0	35.0	41.0	32.0	41.0
23	37.20970832150987	39.0	35.0	41.0	32.0	41.0
24	37.14695509503927	39.0	35.0	41.0	32.0	41.0
25	37.08394761976896	39.0	35.0	41.0	32.0	41.0
26	37.113908796155044	39.0	35.0	41.0	32.0	41.0
27	37.09089092335341	39.0	35.0	41.0	32.0	41.0
28	37.03192440383101	39.0	35.0	41.0	31.0	41.0
29	36.99885001312137	39.0	35.0	41.0	31.0	41.0
30	36.91407635144794	39.0	35.0	41.0	31.0	41.0
31	36.79546164114578	39.0	35.0	41.0	31.0	41.0
32	36.71094507301385	39.0	35.0	41.0	30.0	41.0
33	36.60832421237989	39.0	35.0	41.0	30.0	41.0
34	36.548610236945656	39.0	35.0	41.0	30.0	41.0
35	36.47862140966914	39.0	35.0	41.0	30.0	41.0
36	36.42350702615669	39.0	35.0	41.0	30.0	41.0
37	36.38489961261728	39.0	35.0	41.0	30.0	41.0
38	36.25996957548629	39.0	35.0	41.0	30.0	41.0
39	36.21799541000908	39.0	35.0	41.0	30.0	41.0
40	36.13290207047421	39.0	35.0	41.0	29.0	41.0
41	36.07317564929454	39.0	35.0	41.0	29.0	41.0
42	36.0608817419492	39.0	35.0	41.0	29.0	41.0
43	36.05176896713825	38.0	35.0	41.0	29.0	41.0
44	35.93570812380032	38.0	35.0	40.0	29.0	41.0
45	35.871410558117006	38.0	35.0	40.0	29.0	41.0
46	35.83651837738772	38.0	35.0	40.0	29.0	41.0
47	35.80636055822369	38.0	35.0	40.0	29.0	41.0
48	35.71227072269954	38.0	35.0	40.0	28.0	41.0
49	35.648737325786236	38.0	35.0	40.0	28.0	41.0
50	35.56535332048932	38.0	34.0	40.0	28.0	41.0
51	35.12148256564419	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	4.0
11	6.0
12	11.0
13	14.0
14	29.0
15	74.0
16	175.0
17	424.0
18	886.0
19	1909.0
20	3202.0
21	5052.0
22	7534.0
23	10939.0
24	16016.0
25	22980.0
26	30312.0
27	37070.0
28	42980.0
29	50628.0
30	60831.0
31	75343.0
32	94153.0
33	120907.0
34	195862.0
35	259938.0
36	200585.0
37	269082.0
38	434493.0
39	870514.0
40	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.032924330578915	24.448173426839997	28.22332361142818	12.295578631152908
2	32.01774692181156	26.402973324144817	28.02440504002907	13.55487471401455
3	31.231211369295135	25.54706163062023	29.56362371746593	13.6581032826187
4	28.81631004272091	26.79302298622505	29.40168678965908	14.988980181394961
5	25.356606166120123	30.91231581185731	27.98913024152569	15.741947780496876
6	23.06715795357808	40.796797958613276	25.44351302856192	10.692531059246727
7	87.46891230585526	4.265690351275831	5.934558136921691	2.330839205947217
8	86.82379598080652	4.322300713390129	6.3379780855314305	2.5159252202719147
9	81.15447445884121	6.811343123512289	8.335164635876604	3.699017781769899
10	36.95461854501413	35.51521474600367	18.309789538888687	9.220377170093514
11	30.61073762021701	25.956135503586864	26.43935046010143	16.993776416094697
12	30.312110848209556	23.29036350822095	29.013023939213557	17.38450170435594
13	26.367911881277546	26.800668229852292	27.89632053981821	18.935099349051953
14	18.911630229079947	31.43994429995527	30.381202515036236	19.26722295592855
15	19.038007884201942	29.830033788420906	34.530827400268684	16.60113092710847
16	23.14581506475699	29.188082238641123	31.928279791736454	15.737822904865434
17	22.821123345871534	28.018502200763386	26.99517745143848	22.1651970019266
18	22.73300746815845	29.455594647049328	29.578060782175985	18.233337102616236
19	25.941449523968018	29.43397460925693	26.78505770914364	17.839518157631414
20	26.311728230435467	29.981978560603313	27.42466234692622	16.281630862035
21	25.870793249143198	27.032976958302484	30.356062109248043	16.74016768330627
22	24.975090729484258	24.045322426593216	30.70781443464668	20.271772409275847
23	21.522889859419973	27.53336704352384	30.69654214520558	20.247200951850612
24	20.338090452833114	27.916198173249047	32.4181087729704	19.32760260094744
25	21.03458992691147	29.5162587662497	29.76844512813073	19.680706178708103
26	20.067377709883274	32.784760433623994	26.504886199659627	20.642975656833105
27	19.428235342645593	32.29144664366693	29.723640444547804	18.55667756913967
28	17.575170524492158	31.010886115739744	32.178368156529075	19.235575203239023
29	19.11609604701789	29.130796250345814	31.917043061568034	19.83606464106826
30	20.920053509593536	28.93842058512072	30.158281434574853	19.983244470710893
31	23.58436757477937	28.82039935907967	28.149004731516825	19.446228334624134
32	24.535471441281327	28.325485401851786	27.40126434549958	19.737778811367303
33	23.472889254912335	27.613410966337458	28.10683143411258	20.80686834463763
34	19.487797124392735	28.587699478629947	30.24109898065789	21.68340441631943
35	20.63973976301878	29.087093904216122	28.813216385997325	21.45994994676777
36	23.14311256003294	29.171582736115347	28.482337353664704	19.202967350187006
37	20.82717268934068	31.34997934006257	28.74078214753827	19.082065823058482
38	20.141376556340468	31.660945179691673	27.95730469247274	20.24037357149512
39	21.000381906588636	29.06842528605657	28.214327115438913	21.716865691915885
40	21.556706727743276	27.74590481636125	29.137268037974458	21.56012041792102
41	18.827283634271456	27.779863921775288	29.72250254782189	23.670349896131366
42	19.59714188789868	28.35962230362925	29.3318839373787	22.71135187109337
43	21.054360882524254	27.653095114653762	28.250490895759416	23.042053107062568
44	20.526554598062873	29.086880548580012	28.737475135178574	21.64908971817854
45	20.27931097508504	30.188115664357447	27.595097940904758	21.937475419652756
46	20.67152975279905	30.219798976319655	28.034112721472038	21.074558549409254
47	20.067519946974013	28.764642419509805	29.216174064062162	21.951663569454016
48	19.92688302350539	28.55964321248159	30.362640574694744	21.15083318931828
49	20.99426571168684	27.44126852727005	30.14811148258698	21.41635427845613
50	19.626727202772486	29.420319848545944	29.246577242207717	21.70637570647385
51	18.654287772659615	29.671403872973745	29.207284245890946	22.467024108475695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1443.0
1	3425.0
2	5407.0
3	10651.0
4	15895.0
5	12093.5
6	8292.0
7	8307.5
8	8323.0
9	8428.0
10	8533.0
11	8613.0
12	8693.0
13	8843.5
14	8994.0
15	8680.5
16	8367.0
17	8578.0
18	8789.0
19	9224.5
20	9660.0
21	11954.0
22	14248.0
23	16094.5
24	17941.0
25	21325.0
26	32780.0
27	40851.0
28	49299.5
29	57748.0
30	65341.5
31	72935.0
32	85339.0
33	97743.0
34	101074.5
35	104406.0
36	116872.0
37	129338.0
38	139853.0
39	150368.0
40	168570.5
41	186773.0
42	198705.0
43	210637.0
44	219566.0
45	228495.0
46	230171.5
47	231848.0
48	230011.5
49	228175.0
50	221010.0
51	213845.0
52	196468.5
53	179092.0
54	158545.5
55	137999.0
56	128140.5
57	118282.0
58	105905.0
59	93528.0
60	82389.5
61	71251.0
62	59029.5
63	46808.0
64	37015.0
65	27222.0
66	22146.0
67	17070.0
68	12964.0
69	8858.0
70	6170.5
71	3483.0
72	3146.5
73	2810.0
74	2577.0
75	1442.0
76	540.0
77	391.5
78	243.0
79	163.0
80	83.0
81	90.0
82	97.0
83	54.0
84	11.0
85	11.5
86	12.0
87	10.0
88	8.0
89	5.5
90	3.0
91	2.5
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2812206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.822513889350109
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.76119083067499	11.377938172507344
2	9.96675849373305	2.954648324103126
3	3.562812860423524	1.5842952932634866
4	1.8073922194147785	1.071603851031155
5	0.9785228755447706	0.7252084456904586
6	0.6616932746376375	0.5884774652283563
7	0.45471548979296805	0.4718018664211235
8	0.3449722062265454	0.40906842545861755
9	0.26940099654971184	0.3593880011846578
>10	2.3280985153781115	7.764320248154287
>50	0.8793785636973146	9.617555546773364
>100	1.972178386162023	60.88620122469721
>500	0.007293559112061339	0.754239628126979
>1k	0.0053486100155116486	1.1413054592646705
>5k	2.4311863706871127E-4	0.2939480480951633
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8157	0.29005698729040474	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5559272684860216E-5	0.0	0.0	0.04690268067133062	0.0
2	3.5559272684860216E-5	0.0	0.0	0.15912774526474946	0.0
3	3.5559272684860216E-5	0.0	0.0	0.22597917791228667	0.0
4	3.5559272684860216E-5	0.0	0.0	0.35349472976019536	0.0
5	3.5559272684860216E-5	0.0	0.0	0.6194780894429498	0.0
6	3.5559272684860216E-5	0.0	0.0	0.841759102996011	0.0
7	3.5559272684860216E-5	0.0	0.0	0.9852763275521068	0.0
8	3.5559272684860216E-5	0.0	0.0	1.3121371620713418	0.0
9	3.5559272684860216E-5	0.0	0.0	1.4038445263255963	0.0
10	3.5559272684860216E-5	0.0	0.0	1.6155288766185691	0.0
11	3.5559272684860216E-5	0.0	0.0	1.9731840412828932	0.0
12	3.5559272684860216E-5	0.0	0.0	2.2496573864076814	0.0
13	3.5559272684860216E-5	0.0	0.0	2.357366423370123	0.0
14	3.5559272684860216E-5	0.0	0.0	2.403664596405811	0.0
15	3.5559272684860216E-5	0.0	0.0	2.4921005075730585	0.0
16	3.5559272684860216E-5	0.0	0.0	2.679426756076902	0.0
17	3.5559272684860216E-5	0.0	0.0	2.9001076023591446	0.0
18	3.5559272684860216E-5	0.0	0.0	3.1440797722499703	0.0
19	3.5559272684860216E-5	0.0	0.0	3.30306528042398	0.0
20	3.5559272684860216E-5	0.0	0.0	3.485270993661204	0.0
21	3.5559272684860216E-5	0.0	0.0	3.7064496697610347	0.0
22	3.5559272684860216E-5	0.0	0.0	3.9499240098342723	0.0
23	3.5559272684860216E-5	0.0	0.0	4.181592671376137	0.0
24	3.5559272684860216E-5	0.0	0.0	4.361238116980051	0.0
25	3.5559272684860216E-5	0.0	0.0	4.527726631690566	0.0
26	3.5559272684860216E-5	0.0	0.0	4.684080753685897	0.0
27	3.5559272684860216E-5	0.0	0.0	4.864437384743508	0.0
28	3.5559272684860216E-5	0.0	0.0	5.042838255803451	0.0
29	3.5559272684860216E-5	0.0	0.0	5.2495443079205435	0.0
30	3.5559272684860216E-5	0.0	0.0	5.481604121461941	0.0
31	3.5559272684860216E-5	0.0	0.0	5.690159255758647	0.0
32	3.5559272684860216E-5	0.0	0.0	5.888864471521646	0.0
33	3.5559272684860216E-5	0.0	0.0	6.095997234910956	0.0
34	3.5559272684860216E-5	0.0	0.0	6.308606126293736	0.0
35	3.5559272684860216E-5	0.0	0.0	6.5414482438342	0.0
36	3.5559272684860216E-5	0.0	0.0	6.770129926470536	0.0
37	3.5559272684860216E-5	0.0	0.0	7.0103683727294515	0.0
38	3.5559272684860216E-5	0.0	0.0	7.283925857494081	0.0
39	7.111854536972043E-5	0.0	0.0	7.719491388610934	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	105	0.0	45.000004	2
CTATACG	30	2.1666274E-6	45.000004	1
TAAGCGT	30	2.1666274E-6	45.000004	37
TACCGAA	60	0.0	45.000004	16
GTACCGT	25	3.892251E-5	45.0	39
CGAACCG	40	6.8212103E-9	45.0	5
CGCATTA	50	2.1827873E-11	45.0	37
TAGCGAT	20	7.035031E-4	45.0	1
CGTACAC	20	7.035031E-4	45.0	30
CCGCGAC	20	7.035031E-4	45.0	12
TATCGCG	45	3.8562575E-10	45.0	1
ACGACGT	25	3.892251E-5	45.0	15
CTTACGT	20	7.035031E-4	45.0	35
TTAGTCG	55	1.8189894E-12	44.999996	1
GCGTAAG	115	0.0	43.043476	1
CGATAGA	90	0.0	42.5	10
CCGTCGC	75	0.0	42.0	24
GGACGTA	65	0.0	41.53846	8
CGGTCTA	825	0.0	40.909092	31
CACGCGA	55	6.184564E-11	40.90909	42
>>END_MODULE