FastQCFastQC Report
Thu 26 May 2016
SRR1547556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547556_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2342281
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82230.3510680400857113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT502.1827873E-1145.00000429
GTTTCGA302.166471E-645.00000443
TCGACGA302.166471E-645.00000432
TATCCGT302.166471E-645.00000433
TCGTTAC207.034789E-445.041
GTCGACG207.034789E-445.01
GCGCGTT207.034789E-445.03
AGCCCGT207.034789E-445.018
TAGCGTC207.034789E-445.022
CCGGATA207.034789E-445.039
CGCGATT207.034789E-445.043
CCCGTAT453.8562575E-1045.033
TATCTCG551.8189894E-1245.020
CGCTATC207.034789E-445.045
CGAATCG207.034789E-445.015
CTCGCTA406.8193913E-945.013
CGTCGGA207.034789E-445.023
ACCGTCT453.8562575E-1045.030
ACGTACG207.034789E-445.038
ATACGTA351.2126475E-745.045