##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547556_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2342281
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48440387810002	31.0	31.0	33.0	30.0	34.0
2	31.92368678224346	31.0	31.0	34.0	30.0	34.0
3	32.101276063802764	33.0	31.0	34.0	30.0	34.0
4	35.80895844691563	37.0	35.0	37.0	35.0	37.0
5	35.71670820025437	37.0	35.0	37.0	33.0	37.0
6	35.75495467879388	37.0	35.0	37.0	33.0	37.0
7	36.03442755160461	37.0	35.0	37.0	35.0	37.0
8	35.98850052576954	37.0	35.0	37.0	35.0	37.0
9	37.67888310582718	39.0	38.0	39.0	35.0	39.0
10	37.25249873947661	39.0	37.0	39.0	34.0	39.0
11	37.05790423949987	39.0	37.0	39.0	33.0	39.0
12	36.647799303328675	39.0	35.0	39.0	33.0	39.0
13	36.513414060909	39.0	35.0	39.0	32.0	39.0
14	37.56013817300315	40.0	36.0	41.0	32.0	41.0
15	37.7711944040873	40.0	36.0	41.0	33.0	41.0
16	37.84924951361515	40.0	36.0	41.0	33.0	41.0
17	37.78694571658994	40.0	36.0	41.0	33.0	41.0
18	37.717395137474966	39.0	36.0	41.0	33.0	41.0
19	37.71542654361283	40.0	36.0	41.0	33.0	41.0
20	37.62759207797869	39.0	35.0	41.0	33.0	41.0
21	37.503797793689145	39.0	35.0	41.0	32.0	41.0
22	37.44923815716389	39.0	35.0	41.0	32.0	41.0
23	37.369541058481026	39.0	35.0	41.0	32.0	41.0
24	37.31193994230411	39.0	35.0	41.0	32.0	41.0
25	37.26294539382764	39.0	35.0	41.0	32.0	41.0
26	37.28140816580077	39.0	35.0	41.0	32.0	41.0
27	37.2633714742168	39.0	35.0	41.0	32.0	41.0
28	37.249195976059234	39.0	35.0	41.0	32.0	41.0
29	37.208739258867745	39.0	35.0	41.0	32.0	41.0
30	37.13796423230176	39.0	35.0	41.0	32.0	41.0
31	37.022954974232384	39.0	35.0	41.0	31.0	41.0
32	36.946720312379256	39.0	35.0	41.0	31.0	41.0
33	36.86781005353328	39.0	35.0	41.0	31.0	41.0
34	36.79642408404457	39.0	35.0	41.0	31.0	41.0
35	36.7123735367362	39.0	35.0	41.0	31.0	41.0
36	36.66845566351774	39.0	35.0	41.0	31.0	41.0
37	36.63234855254344	39.0	35.0	41.0	30.0	41.0
38	36.524670609546845	39.0	35.0	41.0	30.0	41.0
39	36.46404252948301	39.0	35.0	41.0	30.0	41.0
40	36.387152950478615	39.0	35.0	41.0	30.0	41.0
41	36.33712522109858	39.0	35.0	41.0	30.0	41.0
42	36.35066629494924	39.0	35.0	41.0	30.0	41.0
43	36.333644853030016	39.0	35.0	41.0	30.0	41.0
44	36.21273152111126	38.0	35.0	41.0	30.0	41.0
45	36.141036024285725	38.0	35.0	40.0	30.0	41.0
46	36.1080843844099	38.0	35.0	40.0	30.0	41.0
47	36.04979376940683	38.0	35.0	40.0	30.0	41.0
48	35.96166429220064	38.0	35.0	40.0	29.0	41.0
49	35.899558165736735	38.0	35.0	40.0	29.0	41.0
50	35.815854289045596	38.0	35.0	40.0	29.0	41.0
51	35.36497414272669	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	7.0
12	8.0
13	5.0
14	21.0
15	58.0
16	119.0
17	234.0
18	557.0
19	1005.0
20	1827.0
21	3065.0
22	4861.0
23	7215.0
24	10549.0
25	15670.0
26	21424.0
27	26351.0
28	31097.0
29	37174.0
30	45476.0
31	57479.0
32	73728.0
33	98520.0
34	167275.0
35	229331.0
36	168211.0
37	227114.0
38	367367.0
39	746281.0
40	247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.519838567618486	23.976755991275173	29.41717923682086	14.086226204285483
2	29.003736101688908	25.90517533976496	31.727790132780825	13.36329842576531
3	29.807183681206485	25.189249283070648	31.784316228496923	13.219250807225949
4	27.80648436289241	27.96667009637187	29.611348937211208	14.615496603524514
5	24.37717763154805	31.617384933746205	28.60971847528115	15.395718959424595
6	22.263511508653316	41.27570517798676	25.63257781623981	10.828205497120114
7	88.86986659585251	3.5339056244746043	5.690606720542924	1.9056210591299676
8	89.06002311422071	2.9087457909618872	5.669857715619945	2.3613733791974574
9	83.78964778350677	5.7406007221166035	7.098934756333676	3.370816738042959
10	39.05983099380476	35.83976474214665	13.801717214971218	11.298687049077373
11	32.245405226785344	24.757789522264833	26.643771605541776	16.353033645408043
12	31.483370270262196	22.377673729155468	28.012693609349178	18.126262391233162
13	25.434949948362302	25.832297661979926	28.629613611688775	20.103138777968997
14	17.690063660167162	30.1530004299228	30.21981564124885	21.93712026866119
15	16.91351293888308	30.890700133758504	34.67897318895555	17.516813738402863
16	22.831334071360352	28.579875770669698	31.780431126752084	16.808359031217858
17	23.744674528803333	27.846360022559207	26.466423114903805	21.942542333733655
18	22.568812196316326	29.599053230590183	29.475071522161517	18.357063050931977
19	23.7338303986584	30.368687616899937	26.895235883312036	19.002246101129625
20	25.272971091000613	30.022998948460923	27.414857568327626	17.28917239221084
21	25.370568262304992	27.858527648902932	30.613833267656616	16.157070821135466
22	24.63316741244966	22.530388113125625	30.65264159167922	22.183802882745496
23	21.509502916174448	26.514068978060273	31.541433329305917	20.43499477645936
24	20.390892467641585	27.379678185495248	33.589863897628	18.639565449235167
25	21.213765555883345	30.12409698067824	29.07375331994752	19.5883841434909
26	19.771154699201336	33.048938193154456	26.44298442415748	20.73692268348674
27	18.349420927719603	33.19016804559317	29.675090221882005	18.78532080480523
28	16.6955203069145	30.9236167650252	32.99104590781379	19.389817020246504
29	17.938667478411002	28.19853809171487	32.5609950300583	21.30179939981582
30	20.8296954976794	28.546788365699932	31.08866954904215	19.53484658757852
31	23.908361123195725	27.601982853466346	28.157210855571986	20.33244516776595
32	24.349341517947675	28.3899754128561	28.237645269717852	19.02303779947837
33	24.000536229427638	27.80802132622004	27.19929846162779	20.99214398272453
34	19.873106599933994	27.07937262864703	31.244287085964494	21.80323368545448
35	20.730732136750458	27.74146227544859	30.20026205224736	21.32754353555359
36	22.98238341172558	28.72567381966553	28.734682132502464	19.557260636106427
37	20.811166550896328	29.72529769058452	29.88390376731058	19.579631991208572
38	20.845321291510285	29.245082037552283	28.85063747688685	21.058959194050587
39	21.373951289362804	28.379942457800748	29.311427621194895	20.93467863164155
40	22.21646335345759	27.285624568529563	29.7371237695221	20.760788308490742
41	18.92394635827213	26.82961608790747	30.79954966974501	23.446887884075394
42	20.345765516605393	26.612861565286146	29.634488773977164	23.406884144131297
43	21.198566696310138	27.998818245974753	28.162291373238308	22.640323684476797
44	20.542027194858345	28.91070712694164	28.76644604127344	21.78081963692657
45	20.37560822121684	30.204403314546802	27.393681629146972	22.026306835089386
46	21.452848740181047	29.65912288064498	28.452393201328107	20.435635177845867
47	20.86141671302461	27.38714953500455	30.831783206199425	20.91965054577141
48	20.637788548854726	27.208178694187417	31.209705411092862	20.944327345864995
49	20.911154554043687	26.915472567125803	30.38798504534682	21.785387833483686
50	19.759414007115286	27.346206539693576	31.26443838292673	21.629941070264415
51	18.99080426302395	28.495214707372856	29.68520856378889	22.828772465814307
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1304.0
1	2862.5
2	4421.0
3	9305.0
4	14189.0
5	10351.0
6	6513.0
7	6471.0
8	6429.0
9	6777.0
10	7125.0
11	7686.0
12	8247.0
13	7727.5
14	7208.0
15	6950.5
16	6693.0
17	6631.5
18	6570.0
19	6926.5
20	7283.0
21	8474.0
22	9665.0
23	12444.0
24	15223.0
25	18892.0
26	27343.5
27	32126.0
28	40396.0
29	48666.0
30	52616.0
31	56566.0
32	66455.5
33	76345.0
34	83203.5
35	90062.0
36	96682.0
37	103302.0
38	112912.5
39	122523.0
40	135617.0
41	148711.0
42	160524.5
43	172338.0
44	177862.5
45	183387.0
46	193203.5
47	203020.0
48	204453.5
49	205887.0
50	197601.0
51	189315.0
52	163904.5
53	138494.0
54	127971.5
55	117449.0
56	107488.5
57	97528.0
58	89564.0
59	81600.0
60	69278.5
61	56957.0
62	48657.0
63	40357.0
64	32788.5
65	25220.0
66	18946.5
67	12673.0
68	9667.0
69	6661.0
70	5599.5
71	4538.0
72	3554.0
73	2570.0
74	2046.5
75	933.5
76	344.0
77	365.5
78	387.0
79	305.0
80	223.0
81	133.0
82	43.0
83	30.5
84	18.0
85	12.0
86	6.0
87	5.0
88	4.0
89	3.5
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2342281.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.426066910532699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.07447453147282	11.581038673955826
2	10.444695722785946	3.222411501597014
3	3.852790403289228	1.7830020766019372
4	1.9560283524984086	1.2069529697815797
5	1.1465737133115963	0.884356140970131
6	0.7218300802875058	0.6680999469930153
7	0.4808151809551902	0.5191961007110256
8	0.37178664981498794	0.4588164589191035
9	0.2833954855997722	0.39345099507044917
>10	2.6837251288533506	9.494594075785882
>50	1.0655756599555395	12.0617185159815
>100	1.905707060650285	55.65401783712849
>500	0.0089614439291753	0.9613284433060643
>1k	0.0033605414734407374	0.7557830906232685
>5k	2.8004512278672814E-4	0.35523317257474346
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8223	0.3510680400857113	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.269342576744635E-5	0.0	0.0	0.08978427438893967	0.0
2	4.269342576744635E-5	0.0	0.0	0.31285742402384686	0.0
3	4.269342576744635E-5	0.0	0.0	0.45071449582693107	0.0
4	4.269342576744635E-5	0.0	0.0	0.6426641380773699	0.0
5	4.269342576744635E-5	0.0	0.0	1.0785640151629972	0.0
6	4.269342576744635E-5	0.0	0.0	1.4174217354792187	0.0
7	4.269342576744635E-5	0.0	0.0	1.6463865778700335	0.0
8	4.269342576744635E-5	0.0	0.0	2.163617431042646	0.0
9	4.269342576744635E-5	0.0	0.0	2.3155633333489876	0.0
10	4.269342576744635E-5	0.0	0.0	2.6466935436013013	0.0
11	4.269342576744635E-5	0.0	0.0	3.177116665336055	0.0
12	4.269342576744635E-5	0.0	0.0	3.5781360135696785	0.0
13	4.269342576744635E-5	0.0	0.0	3.7493366508971384	0.0
14	4.269342576744635E-5	0.0	0.0	3.8201650442453317	0.0
15	4.269342576744635E-5	0.0	0.0	3.939621249542647	0.0
16	4.269342576744635E-5	0.0	0.0	4.200691548110581	0.0
17	4.269342576744635E-5	0.0	0.0	4.486267873069029	0.0
18	4.269342576744635E-5	0.0	0.0	4.790330451384783	0.0
19	4.269342576744635E-5	0.0	0.0	5.000040558754479	0.0
20	4.269342576744635E-5	0.0	0.0	5.220253248862967	0.0
21	4.269342576744635E-5	0.0	0.0	5.495497764785694	0.0
22	4.269342576744635E-5	0.0	0.0	5.795291000524702	0.0
23	4.269342576744635E-5	0.0	0.0	6.090089105448919	0.0
24	4.269342576744635E-5	0.0	0.0	6.312137612865408	0.0
25	4.269342576744635E-5	0.0	0.0	6.518603019876778	0.0
26	4.269342576744635E-5	0.0	0.0	6.701629736141821	0.0
27	4.269342576744635E-5	0.0	0.0	6.907838982598586	0.0
28	4.269342576744635E-5	0.0	0.0	7.114475163313027	0.0
29	4.269342576744635E-5	0.0	0.0	7.345531983566446	0.0
30	4.269342576744635E-5	0.0	0.0	7.61146933267187	0.0
31	4.269342576744635E-5	0.0	0.0	7.855248793803988	0.0
32	4.269342576744635E-5	0.0	0.0	8.078065782884291	0.0
33	4.269342576744635E-5	0.0	0.0	8.296485349110547	0.0
34	4.269342576744635E-5	0.0	0.0	8.535910080814386	0.0
35	4.269342576744635E-5	0.0	0.0	8.802701298435158	0.0
36	4.269342576744635E-5	0.0	0.0	9.037344366453043	0.0
37	4.269342576744635E-5	0.0	0.0	9.296237300306837	0.0
38	4.269342576744635E-5	0.0	0.0	9.569219064663889	0.0
39	4.269342576744635E-5	0.0	0.0	9.976727813614165	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	50	2.1827873E-11	45.000004	29
GTTTCGA	30	2.166471E-6	45.000004	43
TCGACGA	30	2.166471E-6	45.000004	32
TATCCGT	30	2.166471E-6	45.000004	33
TCGTTAC	20	7.034789E-4	45.0	41
GTCGACG	20	7.034789E-4	45.0	1
GCGCGTT	20	7.034789E-4	45.0	3
AGCCCGT	20	7.034789E-4	45.0	18
TAGCGTC	20	7.034789E-4	45.0	22
CCGGATA	20	7.034789E-4	45.0	39
CGCGATT	20	7.034789E-4	45.0	43
CCCGTAT	45	3.8562575E-10	45.0	33
TATCTCG	55	1.8189894E-12	45.0	20
CGCTATC	20	7.034789E-4	45.0	45
CGAATCG	20	7.034789E-4	45.0	15
CTCGCTA	40	6.8193913E-9	45.0	13
CGTCGGA	20	7.034789E-4	45.0	23
ACCGTCT	45	3.8562575E-10	45.0	30
ACGTACG	20	7.034789E-4	45.0	38
ATACGTA	35	1.2126475E-7	45.0	45
>>END_MODULE