FastQCFastQC Report
Thu 26 May 2016
SRR1547555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547555_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2688999
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113120.4206769879795418No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG253.8922037E-545.00000435
GTCGCGA207.034974E-445.0000041
TAGGTCG207.034974E-445.00000432
ATTACGC207.034974E-445.00000439
CGTTACG253.8922037E-545.00000416
CCGTATC253.8922037E-545.00000422
TCGTCCG253.8922037E-545.0000041
ACGACGT207.034974E-445.00000422
GTCGGAC253.8922037E-545.00000412
GTCGACG351.2127384E-745.014
TCCGATC351.2127384E-745.034
GGCCGTA351.2127384E-745.033
CGCTATT351.2127384E-745.032
TTACCGA600.044.99999637
CGGTCTA3450.044.34782431
CACGACG3850.042.6623426
TTGCGCG900.042.51
TATAGCG1750.042.428571
TACGGGA13000.041.8846174
TTTACGG3400.041.6911772