##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547555_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2688999
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.404655040779115	31.0	31.0	33.0	30.0	34.0
2	31.834155758332376	31.0	31.0	34.0	30.0	34.0
3	31.98794346892654	33.0	31.0	34.0	30.0	34.0
4	35.698267645320804	37.0	35.0	37.0	33.0	37.0
5	35.583532756985036	37.0	35.0	37.0	33.0	37.0
6	35.62098535551705	37.0	35.0	37.0	33.0	37.0
7	35.99216139537426	37.0	35.0	37.0	35.0	37.0
8	35.95726179146961	37.0	35.0	37.0	35.0	37.0
9	37.635645085773554	39.0	37.0	39.0	35.0	39.0
10	37.14984683891664	39.0	37.0	39.0	33.0	39.0
11	36.838082498357196	39.0	37.0	39.0	32.0	39.0
12	36.456026945342856	39.0	35.0	39.0	32.0	39.0
13	36.30107783602746	39.0	35.0	39.0	32.0	39.0
14	37.348657251267106	40.0	35.0	41.0	32.0	41.0
15	37.57521032919685	40.0	35.0	41.0	32.0	41.0
16	37.64627841066508	40.0	35.0	41.0	33.0	41.0
17	37.56874621373976	40.0	35.0	41.0	32.0	41.0
18	37.49879973923382	39.0	36.0	41.0	32.0	41.0
19	37.50601580736921	39.0	36.0	41.0	32.0	41.0
20	37.41484247483915	39.0	35.0	41.0	32.0	41.0
21	37.28111092640793	39.0	35.0	41.0	32.0	41.0
22	37.2203277130263	39.0	35.0	41.0	32.0	41.0
23	37.158167407276835	39.0	35.0	41.0	32.0	41.0
24	37.117036860184776	39.0	35.0	41.0	32.0	41.0
25	37.04627298113536	39.0	35.0	41.0	32.0	41.0
26	37.05762292957342	39.0	35.0	41.0	32.0	41.0
27	37.0344012772039	39.0	35.0	41.0	32.0	41.0
28	36.99950762346881	39.0	35.0	41.0	31.0	41.0
29	36.962838959776484	39.0	35.0	41.0	31.0	41.0
30	36.866809545113256	39.0	35.0	41.0	31.0	41.0
31	36.778394859946026	39.0	35.0	41.0	31.0	41.0
32	36.692326772899506	39.0	35.0	41.0	31.0	41.0
33	36.60971870945285	39.0	35.0	41.0	30.0	41.0
34	36.54939514666982	39.0	35.0	41.0	30.0	41.0
35	36.455189830862714	39.0	35.0	41.0	30.0	41.0
36	36.41574950381164	39.0	35.0	41.0	30.0	41.0
37	36.37935343226234	39.0	35.0	41.0	30.0	41.0
38	36.27125632995773	39.0	35.0	41.0	30.0	41.0
39	36.2282016467838	39.0	35.0	41.0	30.0	41.0
40	36.13117781003265	38.0	35.0	41.0	30.0	41.0
41	36.08130014179998	38.0	35.0	41.0	29.0	41.0
42	36.071875073214976	38.0	35.0	41.0	29.0	41.0
43	36.057449258999355	38.0	35.0	41.0	29.0	41.0
44	35.96397618593387	38.0	35.0	40.0	29.0	41.0
45	35.8840419799338	38.0	35.0	40.0	29.0	41.0
46	35.85015130165537	38.0	35.0	40.0	29.0	41.0
47	35.786600515656566	38.0	35.0	40.0	29.0	41.0
48	35.69553726126339	38.0	35.0	40.0	28.0	41.0
49	35.63856066885856	38.0	34.0	40.0	28.0	41.0
50	35.53173876226804	38.0	34.0	40.0	28.0	41.0
51	35.11661514191712	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	3.0
11	6.0
12	7.0
13	14.0
14	26.0
15	60.0
16	157.0
17	375.0
18	793.0
19	1457.0
20	2604.0
21	4426.0
22	6725.0
23	10199.0
24	14644.0
25	21304.0
26	28547.0
27	34440.0
28	40678.0
29	48223.0
30	59212.0
31	72215.0
32	91760.0
33	118222.0
34	195868.0
35	268454.0
36	188241.0
37	250059.0
38	400620.0
39	829426.0
40	228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.03764672281395	22.95679544693025	31.801313425553523	13.20424440470227
2	30.338352673243836	26.40599717590077	29.812394872590136	13.443255278265257
3	29.18193721901719	26.500381740565913	31.198635626119607	13.11904541429729
4	26.812691265411402	27.80566299950279	31.057207533360927	14.32443820172488
5	24.69662502663631	31.780859717686766	29.65125684315985	13.871258412517074
6	23.10778099954667	40.67647477741717	25.486249715972377	10.72949450706378
7	89.2134954308276	3.3322437085324315	5.669135615148983	1.7851252454909803
8	89.456894554442	2.782633983872809	5.426740582648041	2.3337308790371436
9	84.30367582881213	5.646413405137005	6.965268488385455	3.0846422776654063
10	42.72850231628944	34.83880060944612	13.152961380796349	9.27973569346809
11	30.056798087317993	25.099525883051648	28.2334058138363	16.610270215794056
12	30.344079711446525	22.380409959245057	29.078887719928492	18.196622609379922
13	25.491530491457976	26.928496440497003	28.892201149944647	18.687771918100378
14	17.34783092146929	31.8665421593686	30.355310656493362	20.43031626266875
15	17.08676723196996	29.89685009179996	36.29733592314464	16.719046753085443
16	22.104842731440215	28.15527264978529	34.046870229405066	15.693014389369427
17	21.97858757106269	27.527195064036842	28.146533338242225	22.347684026658246
18	22.02395761396713	29.01674563657331	30.638575916168058	18.320720833291496
19	24.234334040287855	30.880785005870216	27.358024305698887	17.526856648143045
20	26.20376578793819	29.546496670322302	27.662375478756225	16.58736206298329
21	25.292013868357703	27.261222484649494	30.865649262048816	16.581114384943987
22	22.76865852311585	23.477918734815447	32.320391342652044	21.43303139941666
23	20.507854409763635	27.214141767996196	31.649621290301706	20.628382531938467
24	20.93816323472043	27.270705567387715	33.23158543383616	18.559545764055695
25	20.53451860710993	29.140471974887312	30.25222396884491	20.072785449157845
26	19.430427456462425	33.616338273089724	27.2706311902682	19.68260308017965
27	18.921725147536314	32.278368270125796	30.320241844641814	18.479664737696073
28	17.269958077336586	30.36338057396079	33.571935132738986	18.794726215963635
29	19.70272209100859	28.745083207543033	32.05865825907708	19.49353644237131
30	21.32563083883631	28.946533635750697	30.573235616673717	19.154599908739275
31	23.27323290190885	29.07461103555635	28.32128238054384	19.33087368199096
32	24.371448260114636	28.764086561579234	28.894060577932535	17.970404600373595
33	22.69662428286511	29.123328048838992	28.85661169825649	19.32343597003941
34	20.376430039579784	28.262970718843704	30.41592800889848	20.944671232678033
35	19.807073189688804	29.072677230448953	30.498263480202116	20.621986099660134
36	23.26486547596336	28.08427968920777	29.23861258408798	19.41224225074089
37	20.237010129048024	30.81715538012472	29.169776559976405	19.77605793085085
38	20.719345749105894	30.128348876291884	28.53649257586187	20.61581279874035
39	20.077954658964174	28.930170669457294	29.37122698818408	21.620647683394452
40	22.23712243849849	27.312988959832264	29.510275013118264	20.93961358855098
41	19.749468110624065	26.983238000460396	29.305663557331187	23.961630331584356
42	20.389892298212086	28.270073733757435	28.396998288210597	22.943035679819886
43	20.397999404239272	28.2706315621538	28.734298525213287	22.597070508393642
44	20.70019364083066	28.878627325633072	28.828162450041816	21.593016583494453
45	19.75281508100226	30.953823337234414	27.6005309038791	21.692830677884224
46	21.472079387162285	29.714849280345586	28.47435049250669	20.338720839985434
47	20.495656562163095	28.418493275750567	29.91369650937021	21.172153652716123
48	20.41547802732541	27.700419375388385	30.706816923323508	21.17728567396269
49	21.578698987987725	26.610050803291486	30.600346076737107	21.210904131983686
50	19.63868339110576	28.77873885412378	29.386102412087173	22.196475342683282
51	18.506440500721645	29.074871355474656	28.90001074749377	23.51867739630993
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1660.0
1	3115.0
2	4570.0
3	11540.5
4	18511.0
5	12838.5
6	7166.0
7	7352.5
8	7539.0
9	8040.0
10	8541.0
11	8661.5
12	8782.0
13	8956.5
14	9131.0
15	9533.0
16	9935.0
17	9708.0
18	9481.0
19	9415.5
20	9350.0
21	10152.5
22	10955.0
23	14058.5
24	17162.0
25	20418.5
26	30969.0
27	38263.0
28	44422.0
29	50581.0
30	61096.5
31	71612.0
32	82653.0
33	93694.0
34	96355.5
35	99017.0
36	109616.0
37	120215.0
38	133752.5
39	147290.0
40	164205.0
41	181120.0
42	199387.0
43	217654.0
44	225576.5
45	233499.0
46	232915.0
47	232331.0
48	233346.5
49	234362.0
50	219194.5
51	204027.0
52	178827.0
53	153627.0
54	138462.5
55	123298.0
56	112655.0
57	102012.0
58	95784.0
59	89556.0
60	75497.0
61	61438.0
62	52837.0
63	44236.0
64	33114.5
65	21993.0
66	16206.5
67	10420.0
68	8371.5
69	6323.0
70	4773.0
71	3223.0
72	2316.5
73	1410.0
74	1006.0
75	587.0
76	572.0
77	335.5
78	99.0
79	64.0
80	29.0
81	23.5
82	18.0
83	13.5
84	9.0
85	7.0
86	5.0
87	4.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2688999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.922648866171382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.44092485457035	12.597376325869442
2	10.79845354633685	3.654768753246433
3	4.103656436362784	2.0833421081991466
4	2.017988595634232	1.3659884967942566
5	1.1826214138030096	1.000654346370175
6	0.7787753746612199	0.7907365326607732
7	0.5297321764456097	0.6275130130571256
8	0.39758176002727713	0.538250921642882
9	0.3102370832135052	0.47250299020386066
>10	2.7800937873384854	10.681006567873952
>50	1.0474966581924685	12.958496329820063
>100	1.6026538307399725	51.327195399665456
>500	0.00689361279330078	0.76937957044777
>1k	0.002668495274826108	0.7070991503406016
>5k	0.0	0.0
>10k+	2.2237460623550901E-4	0.42568949380803
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11312	0.4206769879795418	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1156567927321654E-4	0.0	0.0	0.07939757508277244	0.0
2	1.1156567927321654E-4	0.0	0.0	0.2920045712177654	0.0
3	1.1156567927321654E-4	0.0	0.0	0.43410205805208557	0.0
4	1.1156567927321654E-4	0.0	0.0	0.6314989332461634	0.0
5	1.1156567927321654E-4	0.0	0.0	1.1137973647442785	0.0
6	1.1156567927321654E-4	0.0	0.0	1.4983642611990557	0.0
7	1.1156567927321654E-4	0.0	0.0	1.7613989443655427	0.0
8	1.1156567927321654E-4	0.0	0.0	2.3764977227585433	0.0
9	1.1156567927321654E-4	0.0	0.0	2.5535896443248958	0.0
10	1.1156567927321654E-4	0.0	0.0	2.93655743271009	0.0
11	1.1156567927321654E-4	0.0	0.0	3.5618086879169533	0.0
12	1.1156567927321654E-4	0.0	0.0	4.019860178453023	0.0
13	1.1156567927321654E-4	0.0	0.0	4.192377907169173	0.0
14	1.1156567927321654E-4	0.0	0.0	4.271887047931219	0.0
15	1.1156567927321654E-4	0.0	0.0	4.394125843854907	0.0
16	1.1156567927321654E-4	0.0	0.0	4.675159789944139	0.0
17	1.1156567927321654E-4	0.0	0.0	5.028599861881689	0.0
18	1.1156567927321654E-4	0.0	0.0	5.394721232696628	0.0
19	1.1156567927321654E-4	0.0	0.0	5.658053424341177	0.0
20	1.487542390309554E-4	0.0	0.0	5.915956086261096	0.0
21	1.487542390309554E-4	0.0	0.0	6.23741399680699	0.0
22	1.487542390309554E-4	0.0	0.0	6.60056028284131	0.0
23	1.487542390309554E-4	0.0	0.0	6.963632191756115	0.0
24	1.487542390309554E-4	0.0	0.0	7.229418828344674	0.0
25	1.487542390309554E-4	0.0	0.0	7.458574733571861	0.0
26	1.487542390309554E-4	0.0	0.0	7.671925500902009	0.0
27	1.487542390309554E-4	0.0	0.0	7.911828899899182	0.0
28	1.487542390309554E-4	0.0	0.0	8.15887250236984	0.0
29	1.487542390309554E-4	0.0	0.0	8.42763422373902	0.0
30	1.487542390309554E-4	0.0	0.0	8.73667115532583	0.0
31	1.487542390309554E-4	0.0	0.0	9.029345120619235	0.0
32	1.487542390309554E-4	0.0	0.0	9.292379803785721	0.0
33	1.487542390309554E-4	0.0	0.0	9.565269455288009	0.0
34	1.487542390309554E-4	0.0	0.0	9.842472979722194	0.0
35	1.487542390309554E-4	0.0	0.0	10.139312063708465	0.0
36	1.487542390309554E-4	0.0	0.0	10.416775908060956	0.0
37	1.8594279878869424E-4	0.0	0.0	10.720234555684105	0.0
38	1.8594279878869424E-4	0.0	0.0	11.042622180223942	0.0
39	1.8594279878869424E-4	0.0	0.0	11.480034020094466	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	25	3.8922037E-5	45.000004	35
GTCGCGA	20	7.034974E-4	45.000004	1
TAGGTCG	20	7.034974E-4	45.000004	32
ATTACGC	20	7.034974E-4	45.000004	39
CGTTACG	25	3.8922037E-5	45.000004	16
CCGTATC	25	3.8922037E-5	45.000004	22
TCGTCCG	25	3.8922037E-5	45.000004	1
ACGACGT	20	7.034974E-4	45.000004	22
GTCGGAC	25	3.8922037E-5	45.000004	12
GTCGACG	35	1.2127384E-7	45.0	14
TCCGATC	35	1.2127384E-7	45.0	34
GGCCGTA	35	1.2127384E-7	45.0	33
CGCTATT	35	1.2127384E-7	45.0	32
TTACCGA	60	0.0	44.999996	37
CGGTCTA	345	0.0	44.347824	31
CACGACG	385	0.0	42.66234	26
TTGCGCG	90	0.0	42.5	1
TATAGCG	175	0.0	42.42857	1
TACGGGA	1300	0.0	41.884617	4
TTTACGG	340	0.0	41.691177	2
>>END_MODULE