FastQCFastQC Report
Thu 26 May 2016
SRR1547554_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547554_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1733071
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96070.554333896303152No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20980.12105678301696815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGA351.212411E-745.00000417
CGTGTTA700.045.00000444
GCGTCTA351.212411E-745.0000048
TCTATAC351.212411E-745.00000432
TCGCATC207.0342794E-445.026
CGAACCC551.8189894E-1245.022
GTCGATA207.0342794E-445.023
GTCGACG253.8916296E-545.017
ACGCACC453.8562575E-1045.042
GACGTAT453.8562575E-1045.011
GCACGTT207.0342794E-445.032
CACGCGT253.8916296E-545.027
TAGCCGT406.8193913E-945.028
ACCTCGC253.8916296E-545.040
CTAACGG551.8189894E-1245.02
CTAACCG453.8562575E-1045.041
CCGTAAC207.0342794E-445.011
TACGCAA253.8916296E-545.022
AAGTTCG207.0342794E-445.018
ACAATCG253.8916296E-545.031