FastQCFastQC Report
Thu 26 May 2016
SRR1547553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547553_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2214534
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77300.3490576346987673No Hit
GGGAATGGTTACATCAGTGCGGCAGAACTGCGCCACGTCATGACAAACTTA25120.11343244222034973No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAAG23030.10399479077765346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATG302.1664164E-645.00000429
CGACGTA351.2126111E-745.017
CGATTCG351.2126111E-745.043
CCGTCGA207.0347043E-445.044
CGTTAAC351.2126111E-745.032
CGCCTAT207.0347043E-445.045
GTCGGTA453.8562575E-1045.043
ATTACGA502.1827873E-1144.99999634
CGACATA253.891982E-544.99999624
ATACGTC253.891982E-544.99999628
GCCGTCG253.891982E-544.99999639
TATAGCG1000.042.7499961
TCACGAC7150.042.1678325
CACGACG7150.042.1678326
TAGGGAC20650.041.1864435
ACGGTCT7300.040.99315330
CATTACG556.184564E-1140.90909233
TCTCACG7300.040.68493323
CTATGCG1500.040.5000041
TGCGACA501.0822987E-940.49999630