##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547553_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2214534
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.397326480424326	31.0	31.0	33.0	30.0	34.0
2	31.821797272022014	31.0	31.0	34.0	30.0	34.0
3	31.973488779129152	33.0	31.0	34.0	30.0	34.0
4	35.69371389195199	37.0	35.0	37.0	33.0	37.0
5	35.585690262601524	37.0	35.0	37.0	33.0	37.0
6	35.62497030978075	37.0	35.0	37.0	33.0	37.0
7	35.96147767430981	37.0	35.0	37.0	35.0	37.0
8	35.90810391712207	37.0	35.0	37.0	35.0	37.0
9	37.56365266913942	39.0	37.0	39.0	35.0	39.0
10	37.14522152290279	39.0	37.0	39.0	33.0	39.0
11	36.88659103901769	39.0	37.0	39.0	33.0	39.0
12	36.432005108072396	39.0	35.0	39.0	32.0	39.0
13	36.22989712508365	39.0	35.0	39.0	32.0	39.0
14	37.18352619557884	39.0	35.0	41.0	31.0	41.0
15	37.45422693894065	40.0	35.0	41.0	32.0	41.0
16	37.54209057074762	40.0	35.0	41.0	32.0	41.0
17	37.48407610811123	39.0	35.0	41.0	32.0	41.0
18	37.414643893478264	39.0	36.0	41.0	32.0	41.0
19	37.43617302782437	39.0	36.0	41.0	32.0	41.0
20	37.33101185170334	39.0	35.0	41.0	32.0	41.0
21	37.200216840202046	39.0	35.0	41.0	32.0	41.0
22	37.141813582451206	39.0	35.0	41.0	32.0	41.0
23	37.07755491674546	39.0	35.0	41.0	32.0	41.0
24	37.00144183832806	39.0	35.0	41.0	32.0	41.0
25	36.91415485153987	39.0	35.0	41.0	31.0	41.0
26	36.94398324884603	39.0	35.0	41.0	31.0	41.0
27	36.91022761447781	39.0	35.0	41.0	31.0	41.0
28	36.863099415046236	39.0	35.0	41.0	31.0	41.0
29	36.841406363596136	39.0	35.0	41.0	31.0	41.0
30	36.754403861038035	39.0	35.0	41.0	31.0	41.0
31	36.62454358343561	39.0	35.0	41.0	31.0	41.0
32	36.540336251328725	39.0	35.0	41.0	30.0	41.0
33	36.455967259929174	39.0	35.0	41.0	30.0	41.0
34	36.4041811956827	39.0	35.0	41.0	30.0	41.0
35	36.318725293899305	39.0	35.0	41.0	30.0	41.0
36	36.23658972948711	38.0	35.0	41.0	30.0	41.0
37	36.193088929770326	38.0	35.0	41.0	30.0	41.0
38	36.07211584920349	38.0	35.0	41.0	30.0	41.0
39	36.031183084116115	38.0	35.0	41.0	29.0	41.0
40	35.93986364625695	38.0	35.0	41.0	29.0	41.0
41	35.84665125936201	38.0	35.0	40.0	29.0	41.0
42	35.82797690168677	38.0	35.0	40.0	29.0	41.0
43	35.83248304157895	38.0	35.0	40.0	29.0	41.0
44	35.74718247721643	38.0	35.0	40.0	29.0	41.0
45	35.668501364169614	38.0	34.0	40.0	28.0	41.0
46	35.63154279862039	38.0	34.0	40.0	28.0	41.0
47	35.56162831548308	38.0	34.0	40.0	28.0	41.0
48	35.46080620121434	38.0	34.0	40.0	28.0	41.0
49	35.38907147056672	38.0	34.0	40.0	28.0	41.0
50	35.29633999748931	37.0	34.0	40.0	27.0	41.0
51	34.86263475747042	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	11.0
12	5.0
13	7.0
14	23.0
15	59.0
16	132.0
17	400.0
18	761.0
19	1439.0
20	2577.0
21	4169.0
22	6387.0
23	8975.0
24	13130.0
25	18562.0
26	24715.0
27	30183.0
28	35109.0
29	40845.0
30	49924.0
31	61675.0
32	77420.0
33	101081.0
34	167028.0
35	224410.0
36	157262.0
37	209148.0
38	332473.0
39	646425.0
40	191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.47670435405372	24.147969730877918	29.43291907010685	12.942406844961512
2	31.660430591718168	25.31020070136652	29.460148274986974	13.569220431928342
3	30.123989968092612	25.067079575206343	30.530305698625536	14.278624758075514
4	28.247839048756983	27.74746289738609	28.747266919360914	15.257431134496017
5	24.242526870212874	30.948542673086077	28.571518883882568	16.23741157281848
6	23.682273561841903	39.920949509016346	25.57680306556594	10.819973863575813
7	87.08441595387562	4.230822376174852	6.331896462190239	2.352865207759285
8	86.94696039889205	3.761784646340946	6.250028222641873	3.0412267321251334
9	81.34334356573437	6.618186941361026	8.351734495835242	3.686734997069361
10	40.300939159209115	36.45538971178587	13.72812519473623	9.51554593426879
11	33.06135737812109	22.266716157891455	27.52610707263921	17.145819391348248
12	32.486925014472575	21.247314333399263	28.13395504426665	18.131805607861516
13	26.661997512795015	26.995205311817294	28.11882770822214	18.223969467165553
14	17.712078477910026	31.76379319531784	30.678824529223753	19.84530379754838
15	17.33841069949705	29.242856510669963	36.45909252239974	16.959640267433237
16	23.01527093284637	26.7278352917589	34.139055891668406	16.117837883726327
17	23.56035174894583	26.696542026448906	26.35583829374487	23.387267930860396
18	22.86011413687936	28.46716284328893	30.316355495106418	18.356367524725293
19	25.892445092285783	28.248742173296954	26.784054794372086	19.074757940045174
20	26.79213775900483	28.62137135848896	26.740027473048507	17.846463409457698
21	25.30825898360558	26.729189978568858	30.77184635684076	17.1907046809848
22	23.5892065779979	22.95083299691944	30.449024490028147	23.01093593505451
23	21.102724094549917	26.833907268978486	30.494903216658674	21.568465419812927
24	20.272075298911645	27.1225007157262	33.43466390671807	19.170760078644086
25	22.208329156382337	27.91201218856879	29.870618378403762	20.00904027664511
26	20.345409011557287	31.38339713908208	26.47446370207005	21.79673014729058
27	19.483105700793033	30.96240563477463	30.16752057091921	19.386968093513126
28	17.30513056019912	29.184017946890854	32.8712496624572	20.63960183045282
29	19.694707780508224	27.11839149906933	32.90651667574307	20.28038404467938
30	21.581696194323502	27.86852674196919	30.5656178681384	19.98415919556891
31	24.31233839715263	26.58550286425948	28.242781551333145	20.859377187254747
32	25.651762402383525	27.29201719187874	27.628656864152912	19.42756354158482
33	25.02386506596873	26.05676860233349	28.382359448985657	20.53700688271212
34	21.15840172243912	26.55407413026849	29.90484679846866	22.382677348823727
35	20.40962116634922	27.6631110653528	29.803200131494933	22.124067636803048
36	25.42629736097978	26.845783356679103	28.088482723679114	19.639436558662002
37	20.991233370090505	29.79606544762916	28.99160726365005	20.221093918630284
38	21.444376108020922	29.424203918296126	27.93991873685389	21.19150123682906
39	20.51515126884482	28.97246102340267	28.027476661004076	22.484911046748437
40	24.20333126517814	25.56077260498145	29.062818633626758	21.17307749621365
41	20.061376343736427	25.3698520772316	29.493112320695914	25.075659258336064
42	20.724269756075092	26.200907278912855	29.493202633149906	23.581620331862144
43	21.31360367463313	26.43201684869142	29.054780825221016	23.199598651454437
44	22.00002348123804	25.83563855872161	29.831964648093006	22.332373311947347
45	20.17566675426975	28.935749010852845	27.27404501353332	23.61453922134408
46	21.766746412563545	28.800957673262182	28.130613483468757	21.30168243070551
47	21.222929970820044	27.11604337526541	29.299708200461133	22.361318453453414
48	20.458028641691662	26.766218084707667	30.91201128544425	21.863741988156423
49	22.0540754849553	25.529659964579455	31.322120139045058	21.09414441142019
50	19.84304598619845	28.199205792279546	29.593901019356668	22.36384720216533
51	19.49136929033377	28.59197465471291	28.24892279820495	23.66773325674837
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1831.0
1	2958.0
2	4085.0
3	8869.0
4	13653.0
5	9864.0
6	6075.0
7	6083.0
8	6091.0
9	6593.5
10	7096.0
11	6961.5
12	6827.0
13	6757.5
14	6688.0
15	6283.0
16	5878.0
17	6063.5
18	6249.0
19	7073.0
20	7897.0
21	8527.0
22	9157.0
23	10821.0
24	12485.0
25	14212.0
26	21074.0
27	26209.0
28	31213.5
29	36218.0
30	41350.5
31	46483.0
32	51790.5
33	57098.0
34	67736.0
35	78374.0
36	84679.0
37	90984.0
38	96492.0
39	102000.0
40	115026.0
41	128052.0
42	146769.0
43	165486.0
44	172918.5
45	180351.0
46	186695.5
47	193040.0
48	196834.5
49	200629.0
50	189759.5
51	178890.0
52	161580.0
53	144270.0
54	128452.0
55	112634.0
56	107909.5
57	103185.0
58	94233.5
59	85282.0
60	73285.0
61	61288.0
62	53286.0
63	45284.0
64	36930.0
65	28576.0
66	22569.5
67	16563.0
68	13672.0
69	10781.0
70	8212.0
71	5643.0
72	4500.5
73	3358.0
74	2677.0
75	1508.5
76	1021.0
77	803.5
78	586.0
79	361.5
80	137.0
81	115.0
82	93.0
83	63.5
84	34.0
85	25.0
86	16.0
87	14.0
88	12.0
89	7.5
90	3.0
91	3.5
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2214534.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.79079654547442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.84058759161762	12.734238761400832
2	9.98917151673556	3.354522931907098
3	3.7379394044246887	1.8828894011702033
4	1.8749427348590093	1.2592712798173207
5	1.0835417454039078	0.909676449780263
6	0.7242720027219411	0.7296662304772445
7	0.5125344417120645	0.6024103073334914
8	0.3975340763663435	0.5339931036928293
9	0.30778481526542323	0.4651156991617336
>10	2.7319263783485463	10.214670760198043
>50	1.0591684837905477	13.001059790030995
>100	1.7288948602289227	52.20799345748984
>500	0.008164150134071853	0.8807556013513101
>1k	0.0032656600536287413	0.8705201017558397
>5k	2.7213833780239516E-4	0.3532161244329768
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7730	0.3490576346987673	No Hit
GGGAATGGTTACATCAGTGCGGCAGAACTGCGCCACGTCATGACAAACTTA	2512	0.11343244222034973	No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAAG	2303	0.10399479077765346	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.515622699854687E-5	0.0	0.0	0.07310793151064739	0.0
2	4.515622699854687E-5	0.0	0.0	0.2859292293547988	0.0
3	4.515622699854687E-5	0.0	0.0	0.42406212774335367	0.0
4	4.515622699854687E-5	0.0	0.0	0.6548104477059282	0.0
5	4.515622699854687E-5	0.0	0.0	1.1291314560986645	0.0
6	4.515622699854687E-5	0.0	0.0	1.5392854659264659	0.0
7	4.515622699854687E-5	0.0	0.0	1.8067909546658574	0.0
8	4.515622699854687E-5	0.0	0.0	2.417980487091189	0.0
9	4.515622699854687E-5	0.0	0.0	2.597973207907397	0.0
10	4.515622699854687E-5	0.0	0.0	2.923368979658926	0.0
11	4.515622699854687E-5	0.0	0.0	3.511935242357986	0.0
12	4.515622699854687E-5	0.0	0.0	3.9862562507507224	0.0
13	4.515622699854687E-5	0.0	0.0	4.15974647487914	0.0
14	4.515622699854687E-5	0.0	0.0	4.238453778537607	0.0
15	4.515622699854687E-5	0.0	0.0	4.3629494963726	0.0
16	4.515622699854687E-5	0.0	0.0	4.642692322628598	0.0
17	4.515622699854687E-5	0.0	0.0	4.9555346632745305	0.0
18	4.515622699854687E-5	0.0	0.0	5.298225269966503	0.0
19	4.515622699854687E-5	0.0	0.0	5.535385774162871	0.0
20	4.515622699854687E-5	0.0	0.0	5.800317357963346	0.0
21	4.515622699854687E-5	0.0	0.0	6.110721262351357	0.0
22	4.515622699854687E-5	0.0	0.0	6.4600046781851175	0.0
23	4.515622699854687E-5	0.0	0.0	6.779710765334829	0.0
24	4.515622699854687E-5	0.0	0.0	7.018812987292135	0.0
25	4.515622699854687E-5	0.0	0.0	7.23371147157822	0.0
26	4.515622699854687E-5	0.0	0.0	7.448926049453293	0.0
27	4.515622699854687E-5	0.0	0.0	7.6822482743547855	0.0
28	4.515622699854687E-5	0.0	0.0	7.928981898674845	0.0
29	4.515622699854687E-5	0.0	0.0	8.204886445635967	0.0
30	4.515622699854687E-5	0.0	0.0	8.51583222474796	0.0
31	4.515622699854687E-5	0.0	0.0	8.800542235973799	0.0
32	4.515622699854687E-5	0.0	0.0	9.053913825662645	0.0
33	4.515622699854687E-5	0.0	0.0	9.308053071210466	0.0
34	4.515622699854687E-5	0.0	0.0	9.58233199399964	0.0
35	4.515622699854687E-5	0.0	0.0	9.880543717098044	0.0
36	4.515622699854687E-5	0.0	0.0	10.163673260378934	0.0
37	4.515622699854687E-5	0.0	0.0	10.459762640808405	0.0
38	4.515622699854687E-5	0.0	0.0	10.791344815658734	0.0
39	4.515622699854687E-5	0.0	0.0	11.385465294278616	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTATG	30	2.1664164E-6	45.000004	29
CGACGTA	35	1.2126111E-7	45.0	17
CGATTCG	35	1.2126111E-7	45.0	43
CCGTCGA	20	7.0347043E-4	45.0	44
CGTTAAC	35	1.2126111E-7	45.0	32
CGCCTAT	20	7.0347043E-4	45.0	45
GTCGGTA	45	3.8562575E-10	45.0	43
ATTACGA	50	2.1827873E-11	44.999996	34
CGACATA	25	3.891982E-5	44.999996	24
ATACGTC	25	3.891982E-5	44.999996	28
GCCGTCG	25	3.891982E-5	44.999996	39
TATAGCG	100	0.0	42.749996	1
TCACGAC	715	0.0	42.16783	25
CACGACG	715	0.0	42.16783	26
TAGGGAC	2065	0.0	41.186443	5
ACGGTCT	730	0.0	40.993153	30
CATTACG	55	6.184564E-11	40.909092	33
TCTCACG	730	0.0	40.684933	23
CTATGCG	150	0.0	40.500004	1
TGCGACA	50	1.0822987E-9	40.499996	30
>>END_MODULE