FastQCFastQC Report
Thu 26 May 2016
SRR1547552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547552_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1880586
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70660.3757339467591485No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC70200.37328790068627543No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT49940.26555552365060675Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC43590.23178945286203342Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC36550.19435431296415054Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35250.18744157406255285No Hit
GAGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT28030.1490492857013718No Hit
AAGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT27890.14830483689658436No Hit
TCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC21340.11347526781545753Illumina Single End Adapter 1 (95% over 21bp)
AAGAGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT18860.10028788898779424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC302.1662418E-645.0000046
CTATGCG207.034432E-445.00000415
TCGTTGA1200.045.00000424
GTCGCAA406.8193913E-945.00000428
GTCGACG207.034432E-445.0000049
GTCGAAC351.2124838E-745.00000436
CACGGAT302.1662418E-645.00000439
CAACGAA302.1662418E-645.00000431
TCCGATC406.8193913E-945.00000413
TTAGCCG302.1662418E-645.0000041
GAGTCGT302.1662418E-645.00000438
GCGAAGT302.1662418E-645.00000445
AACCCGC351.2124838E-745.00000421
GAACTCG302.1662418E-645.00000421
CGCATAT351.2124838E-745.00000427
ACTCTCG207.034432E-445.00000442
CCGGATC207.034432E-445.00000439
GTTAGTC600.045.00000433
ACGGGTC351.2124838E-745.0000045
CCGTCGC406.8193913E-945.00000426