##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547552_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1880586 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24135455650526 31.0 31.0 33.0 30.0 34.0 2 31.639989343747107 31.0 31.0 34.0 30.0 34.0 3 31.776620159886335 31.0 31.0 34.0 30.0 34.0 4 35.51210580106414 37.0 35.0 37.0 33.0 37.0 5 35.39715865161178 37.0 35.0 37.0 33.0 37.0 6 35.44561695131198 37.0 35.0 37.0 33.0 37.0 7 35.85842551204784 37.0 35.0 37.0 35.0 37.0 8 35.80624922231687 37.0 35.0 37.0 35.0 37.0 9 37.464194671235454 39.0 37.0 39.0 35.0 39.0 10 36.93194089501889 39.0 37.0 39.0 32.0 39.0 11 36.647390760114135 39.0 35.0 39.0 32.0 39.0 12 35.899082520023015 37.0 35.0 39.0 32.0 39.0 13 35.48971224926699 37.0 35.0 39.0 30.0 39.0 14 36.50092683876196 38.0 35.0 41.0 31.0 41.0 15 36.81901705106812 38.0 35.0 41.0 31.0 41.0 16 37.00379030791466 38.0 35.0 41.0 32.0 41.0 17 36.922745357032326 38.0 35.0 41.0 32.0 41.0 18 36.870597250006114 38.0 35.0 41.0 32.0 41.0 19 36.86396527465375 38.0 35.0 41.0 32.0 41.0 20 36.7180267214581 38.0 35.0 40.0 32.0 41.0 21 36.55353544054885 38.0 35.0 40.0 31.0 41.0 22 36.460646840931496 38.0 35.0 40.0 31.0 41.0 23 36.39124719635262 38.0 35.0 40.0 31.0 41.0 24 36.29441727206307 38.0 35.0 40.0 31.0 41.0 25 36.254198957133575 38.0 35.0 40.0 31.0 41.0 26 36.23878886687447 38.0 35.0 40.0 31.0 41.0 27 36.1897195874052 38.0 35.0 40.0 31.0 41.0 28 36.16779557010421 38.0 35.0 40.0 31.0 41.0 29 36.192823938921165 37.0 35.0 40.0 31.0 41.0 30 36.12676899647238 37.0 35.0 40.0 30.0 41.0 31 35.99613950119803 37.0 35.0 40.0 30.0 41.0 32 35.77741459311087 37.0 34.0 40.0 30.0 41.0 33 35.658912168866514 37.0 34.0 40.0 30.0 41.0 34 35.60014857071147 37.0 34.0 40.0 29.0 41.0 35 35.50020950916363 37.0 34.0 40.0 29.0 41.0 36 35.38757280975185 37.0 34.0 40.0 28.0 41.0 37 35.3246264728122 37.0 34.0 40.0 28.0 41.0 38 35.23645289287488 37.0 34.0 40.0 27.0 41.0 39 35.21947414263426 37.0 34.0 40.0 27.0 41.0 40 35.135038759195275 37.0 34.0 40.0 27.0 41.0 41 35.11094148313345 37.0 34.0 40.0 27.0 41.0 42 35.053059525062935 37.0 34.0 40.0 27.0 41.0 43 34.982377301543245 37.0 34.0 40.0 27.0 41.0 44 34.88115140706142 36.0 34.0 40.0 27.0 41.0 45 34.829038395478854 36.0 34.0 40.0 27.0 41.0 46 34.74685603317264 36.0 34.0 40.0 26.0 41.0 47 34.660938133113824 36.0 34.0 40.0 26.0 41.0 48 34.582421649422045 36.0 34.0 40.0 26.0 41.0 49 34.59554255960642 36.0 34.0 40.0 26.0 41.0 50 34.45734201998739 36.0 34.0 40.0 26.0 41.0 51 33.992584226406024 35.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 3.0 11 2.0 12 3.0 13 5.0 14 14.0 15 43.0 16 129.0 17 334.0 18 746.0 19 1560.0 20 2698.0 21 4285.0 22 6685.0 23 9767.0 24 14314.0 25 20095.0 26 25621.0 27 30557.0 28 34906.0 29 41350.0 30 49790.0 31 61497.0 32 76766.0 33 98959.0 34 171268.0 35 278640.0 36 132173.0 37 158918.0 38 229814.0 39 429507.0 40 135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.325023157675318 25.855876838389737 30.424133754053255 12.394966249881687 2 28.62107874885807 27.256982663914332 31.144972896746015 12.976965690481585 3 28.243855904489344 25.75106908165859 31.82582450363876 14.179250510213306 4 25.699755289042887 28.67037189471792 30.826189283553106 14.803683532686088 5 23.881385908434925 31.421216578236784 30.097161204007687 14.600236309320605 6 20.845576857426355 41.72890790423836 25.56538227977875 11.860132958556536 7 86.10002414141124 4.101327990317912 7.336117571863239 2.4625302964076092 8 85.65782155136749 3.831677998240974 6.420764591462448 4.089735858929078 9 81.19623351444709 5.768786963212531 9.265888398616177 3.769091123724201 10 45.515387225045814 28.594703991202742 14.422791619208056 11.467117164543392 11 38.64364618262605 22.92221679838093 24.17395428871639 14.260182730276627 12 37.44747647807651 22.556267035913276 24.442700307244657 15.553556178765554 13 21.692174673213565 39.420318985677866 24.73782108342825 14.14968525768032 14 14.408168517685443 42.56183976696625 25.922451831503583 17.107539883844716 15 11.402509643270768 29.737645606209977 43.45810295301571 15.401741797503544 16 13.786553765687929 25.08329850376425 41.99042213437726 19.139725596170557 17 15.105557523027397 27.661484239487056 25.482482587874205 31.750475649611342 18 17.78573274500608 27.78405241770384 33.12031462533487 21.30990021195521 19 23.81220534450432 30.775354065169047 24.34799578429277 21.064444806033862 20 26.20938367083452 26.788724365703033 26.556562688438607 20.44532927502385 21 19.419904221343774 33.18034910394951 28.073802527510043 19.325944147196672 22 20.709449075979506 26.593040679873187 26.051028775073306 26.646481469074 23 17.53251380154909 32.50018877094693 25.393520955702105 24.573776471801875 24 17.76371833035022 27.761080854584684 35.91673021068964 18.558470604375447 25 15.377334511689442 30.019844878139047 31.222980496504814 23.3798401136667 26 15.267475138068665 36.942687013516 25.580803005020776 22.20903484339456 27 17.157311603936222 37.03446691616337 25.937553507257842 19.87066797264257 28 12.68758780507778 32.98519716726595 34.52588714368819 19.80132788396808 29 13.193228068272337 27.43198130795401 34.66132365124488 24.713466972528774 30 15.489480406639208 34.92602837626144 28.54195447589209 21.042536741207265 31 23.545745847305042 33.078625492266774 22.54371775606114 20.831910904367042 32 24.13577470001372 33.2227826858224 23.522986983844397 19.118455630319488 33 22.50351752060262 32.2376642174301 22.977412359764458 22.281405902202824 34 16.357773587594505 32.02049786609067 27.29734242411674 24.32438612219808 35 17.387718509017933 28.930184527588743 28.305857854945216 25.37623910844811 36 24.42063271767417 28.539827479306982 27.060288654706564 19.979251148312283 37 16.7020811598087 32.863373437853944 30.316507726846844 20.118037675490513 38 17.22707709192773 34.20433843493464 25.63770016367239 22.93088430946524 39 16.62816802847623 33.287337032180396 29.113265758651824 20.97122918069155 40 21.757739342949485 28.818464031955997 26.852481088341612 22.571315536752905 41 17.238137474170284 25.17970462398423 28.183715076045445 29.398442825800043 42 20.111284461332797 26.953832475621958 24.916754671150375 28.018128391894866 43 19.7435267517678 28.53552031122214 26.055920867219047 25.66503206979101 44 17.635566786097527 31.996250105020458 26.679237216484648 23.68894589239737 45 16.77131489865393 36.21722165325064 22.978635382800896 24.03282806529454 46 21.865950294216802 33.47243890999933 24.45727023385264 20.20434056193123 47 19.926554807916254 28.9954301478369 26.933838707721954 24.144176336524893 48 19.76368004441169 26.14780711969567 30.825444834748318 23.263068001144322 49 19.84392099058485 24.889369590117123 30.77668343803474 24.490025981263287 50 17.521187544733397 30.07833728422949 29.93215944391801 22.4683157271191 51 15.991398425809827 32.390223047496896 24.93728018819666 26.681098338496618 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4125.0 1 3829.5 2 3534.0 3 7305.5 4 11077.0 5 8206.5 6 5336.0 7 6118.5 8 6901.0 9 7948.5 10 8996.0 11 9948.5 12 10901.0 13 10796.0 14 10691.0 15 10204.0 16 9717.0 17 9391.0 18 9065.0 19 8994.0 20 8923.0 21 9256.5 22 9590.0 23 9027.5 24 8465.0 25 9978.0 26 13177.0 27 14863.0 28 19767.5 29 24672.0 30 28811.5 31 32951.0 32 39400.5 33 45850.0 34 53316.0 35 60782.0 36 63686.5 37 66591.0 38 75606.5 39 84622.0 40 106699.5 41 128777.0 42 159129.5 43 189482.0 44 194712.5 45 199943.0 46 207877.0 47 215811.0 48 210811.0 49 205811.0 50 181019.0 51 156227.0 52 133120.5 53 110014.0 54 92548.5 55 75083.0 56 60790.0 57 46497.0 58 41474.5 59 36452.0 60 31172.0 61 25892.0 62 21807.5 63 17723.0 64 13212.5 65 8702.0 66 7168.5 67 5635.0 68 4534.5 69 3434.0 70 2762.5 71 2091.0 72 1889.0 73 1687.0 74 1340.5 75 779.5 76 565.0 77 502.5 78 440.0 79 265.0 80 90.0 81 63.5 82 37.0 83 29.5 84 22.0 85 14.5 86 7.0 87 12.0 88 17.0 89 9.0 90 1.0 91 1.0 92 1.0 93 2.5 94 4.0 95 3.5 96 3.0 97 2.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1880586.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.16081546460025 #Duplication Level Percentage of deduplicated Percentage of total 1 73.4029899028266 14.064611440779762 2 11.194029535230843 4.289734684596862 3 4.5974419757078815 2.642722119172377 4 2.436068877797947 1.867082649061691 5 1.5183841229501345 1.454673899211321 6 1.0163964055383574 1.1684990379242077 7 0.7226019089880175 0.9691949282741088 8 0.5296182972065361 0.8118334767560199 9 0.4284483385838988 0.7388477596548587 >10 2.5934060780782087 9.627675307426198 >50 0.43233553658480284 5.967846122451853 >100 1.0979595046542843 50.418100363244214 >500 0.022739636889405484 2.6061912135718983 >1k 0.007018406447347371 2.6152819527895175 >5k 5.614725157877898E-4 0.7577050450851138 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7066 0.3757339467591485 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 7020 0.37328790068627543 No Hit CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT 4994 0.26555552365060675 Illumina Single End Adapter 1 (95% over 22bp) GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC 4359 0.23178945286203342 Illumina Single End Adapter 1 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC 3655 0.19435431296415054 Illumina Single End Adapter 1 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3525 0.18744157406255285 No Hit GAGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT 2803 0.1490492857013718 No Hit AAGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT 2789 0.14830483689658436 No Hit TCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC 2134 0.11347526781545753 Illumina Single End Adapter 1 (95% over 21bp) AAGAGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT 1886 0.10028788898779424 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.3174914627674565E-5 0.0 0.0 0.2845389681726866 0.0 2 5.3174914627674565E-5 0.0 0.0 0.9656564496385701 0.0 3 5.3174914627674565E-5 0.0 0.0 1.4090288878041206 0.0 4 5.3174914627674565E-5 0.0 0.0 2.206705782133867 0.0 5 5.3174914627674565E-5 0.0 0.0 3.4129255455480365 0.0 6 5.3174914627674565E-5 0.0 0.0 4.636958905362477 0.0 7 5.3174914627674565E-5 0.0 0.0 5.2735158083703695 0.0 8 5.3174914627674565E-5 0.0 0.0 6.812131963122133 0.0 9 5.3174914627674565E-5 0.0 0.0 7.197650094172774 0.0 10 5.3174914627674565E-5 0.0 0.0 8.085086244394034 0.0 11 5.3174914627674565E-5 0.0 0.0 9.325284778255288 0.0 12 5.3174914627674565E-5 0.0 0.0 10.252070365301028 0.0 13 5.3174914627674565E-5 0.0 0.0 10.614085184086237 0.0 14 5.3174914627674565E-5 0.0 0.0 10.751489163484148 0.0 15 5.3174914627674565E-5 0.0 0.0 10.974717455091126 0.0 16 5.3174914627674565E-5 0.0 0.0 11.579263059493158 0.0 17 5.3174914627674565E-5 0.0 0.0 12.233048634840417 0.0 18 5.3174914627674565E-5 0.0 0.0 13.014613530037977 0.0 19 5.3174914627674565E-5 0.0 0.0 13.413106340257771 0.0 20 5.3174914627674565E-5 0.0 0.0 13.786713290431813 0.0 21 5.3174914627674565E-5 0.0 0.0 14.334149036523723 0.0 22 5.3174914627674565E-5 0.0 0.0 14.870099001056055 0.0 23 5.3174914627674565E-5 0.0 0.0 15.509261474880702 0.0 24 5.3174914627674565E-5 0.0 0.0 15.920622614440392 0.0 25 5.3174914627674565E-5 0.0 0.0 16.275086595348473 0.0 26 5.3174914627674565E-5 0.0 0.0 16.622318787867187 0.0 27 5.3174914627674565E-5 0.0 0.0 16.942910348157437 0.0 28 5.3174914627674565E-5 0.0 0.0 17.305669615747433 0.0 29 5.3174914627674565E-5 0.0 0.0 17.69645206334621 0.0 30 5.3174914627674565E-5 0.0 0.0 18.13461335987825 0.0 31 5.3174914627674565E-5 0.0 0.0 18.54374115302358 0.0 32 5.3174914627674565E-5 0.0 0.0 18.89448288990772 0.0 33 5.3174914627674565E-5 0.0 0.0 19.234164244549305 0.0 34 5.3174914627674565E-5 0.0 0.0 19.58602265464063 0.0 35 5.3174914627674565E-5 0.0 0.0 20.00424335818729 0.0 36 5.3174914627674565E-5 0.0 0.0 20.40986160696719 0.0 37 5.3174914627674565E-5 0.0 0.0 20.782245534104796 0.0 38 5.3174914627674565E-5 0.0 0.0 21.140750808524576 0.0 39 5.3174914627674565E-5 0.0 0.0 21.508402168260318 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTGC 30 2.1662418E-6 45.000004 6 CTATGCG 20 7.034432E-4 45.000004 15 TCGTTGA 120 0.0 45.000004 24 GTCGCAA 40 6.8193913E-9 45.000004 28 GTCGACG 20 7.034432E-4 45.000004 9 GTCGAAC 35 1.2124838E-7 45.000004 36 CACGGAT 30 2.1662418E-6 45.000004 39 CAACGAA 30 2.1662418E-6 45.000004 31 TCCGATC 40 6.8193913E-9 45.000004 13 TTAGCCG 30 2.1662418E-6 45.000004 1 GAGTCGT 30 2.1662418E-6 45.000004 38 GCGAAGT 30 2.1662418E-6 45.000004 45 AACCCGC 35 1.2124838E-7 45.000004 21 GAACTCG 30 2.1662418E-6 45.000004 21 CGCATAT 35 1.2124838E-7 45.000004 27 ACTCTCG 20 7.034432E-4 45.000004 42 CCGGATC 20 7.034432E-4 45.000004 39 GTTAGTC 60 0.0 45.000004 33 ACGGGTC 35 1.2124838E-7 45.000004 5 CCGTCGC 40 6.8193913E-9 45.000004 26 >>END_MODULE