FastQCFastQC Report
Thu 26 May 2016
SRR1547551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547551_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2026005
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258141.2741330845679057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTA207.034564E-445.036
CGTTTTT138650.043.377211
CGACGGT4900.041.78571328
CCGAACG650.041.53846436
TCACGAC5000.040.94999725
CGACGTT1550.040.6451627
CGGTCTA5050.040.54455631
TAATGCG501.0822987E-940.51
TCTCACG5100.040.1470623
ACGGTCT5100.040.1470630
TAGGGAT33150.040.1131255
GTCGTTA451.929584E-840.036
AGTACGG2150.039.767442
CACGACG5100.039.70588326
ATAGGGA30950.039.474964
GACGGTC5200.039.37500429
TACGGGA9500.039.315794
ACGACGG5250.038.99999627
TACCCGT356.2506497E-638.57142627
CGAACGT700.038.57142637
Produced by FastQC (version 0.11.5)