##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547551_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2026005
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.468590156490237	31.0	31.0	33.0	30.0	34.0
2	31.89629245732365	31.0	31.0	34.0	30.0	34.0
3	31.916890136006575	33.0	31.0	34.0	30.0	34.0
4	35.72342318997239	37.0	35.0	37.0	33.0	37.0
5	35.6710422728473	37.0	35.0	37.0	33.0	37.0
6	35.728285468199736	37.0	35.0	37.0	33.0	37.0
7	36.04842189431911	37.0	35.0	37.0	35.0	37.0
8	36.004022201327246	37.0	35.0	37.0	35.0	37.0
9	37.68265872986493	39.0	38.0	39.0	35.0	39.0
10	37.25111438520636	39.0	37.0	39.0	34.0	39.0
11	37.0538838749164	39.0	37.0	39.0	33.0	39.0
12	36.782582471415424	39.0	35.0	39.0	33.0	39.0
13	36.70151307622637	39.0	35.0	39.0	33.0	39.0
14	37.853601052317245	40.0	37.0	41.0	33.0	41.0
15	38.010226035967335	40.0	37.0	41.0	33.0	41.0
16	38.04160108193218	40.0	36.0	41.0	33.0	41.0
17	37.96870935659093	40.0	36.0	41.0	33.0	41.0
18	37.816310423715635	39.0	36.0	41.0	33.0	41.0
19	37.72819662340419	39.0	36.0	41.0	33.0	41.0
20	37.55456477155782	39.0	35.0	41.0	33.0	41.0
21	37.437612444194365	39.0	35.0	41.0	33.0	41.0
22	37.382539036182045	39.0	35.0	41.0	33.0	41.0
23	37.28551015421976	39.0	35.0	41.0	32.0	41.0
24	37.21513865957883	39.0	35.0	41.0	32.0	41.0
25	37.12768922090518	39.0	35.0	41.0	32.0	41.0
26	37.13420746740506	39.0	35.0	41.0	32.0	41.0
27	37.102302314160134	39.0	35.0	41.0	32.0	41.0
28	37.03977927004129	39.0	35.0	41.0	32.0	41.0
29	36.97424290660685	39.0	35.0	41.0	32.0	41.0
30	36.86070271297455	39.0	35.0	41.0	31.0	41.0
31	36.687073822621365	39.0	35.0	41.0	31.0	41.0
32	36.44271805844507	39.0	35.0	41.0	30.0	41.0
33	36.19160219249212	39.0	35.0	41.0	30.0	41.0
34	35.98568759701975	39.0	35.0	41.0	29.0	41.0
35	35.78374485749048	39.0	35.0	41.0	27.0	41.0
36	35.70202492096515	39.0	35.0	41.0	27.0	41.0
37	35.64929800271964	39.0	35.0	41.0	26.0	41.0
38	35.52415961461102	39.0	35.0	41.0	26.0	41.0
39	35.44444559613624	38.0	35.0	41.0	25.0	41.0
40	35.371413693450904	38.0	35.0	41.0	25.0	41.0
41	35.30319026853339	38.0	35.0	41.0	24.0	41.0
42	35.27057485050629	38.0	35.0	41.0	24.0	41.0
43	35.21562928028312	38.0	35.0	40.0	24.0	41.0
44	35.09809847458422	38.0	34.0	40.0	24.0	41.0
45	35.029763993672276	38.0	34.0	40.0	24.0	41.0
46	34.979490178948225	38.0	34.0	40.0	24.0	41.0
47	34.929705504181875	38.0	34.0	40.0	23.0	41.0
48	34.83776989691536	38.0	34.0	40.0	23.0	41.0
49	34.759744423138144	38.0	34.0	40.0	24.0	41.0
50	34.66929745977922	38.0	34.0	40.0	24.0	41.0
51	34.26228612466406	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	7.0
12	3.0
13	12.0
14	21.0
15	49.0
16	143.0
17	320.0
18	646.0
19	1356.0
20	2425.0
21	3896.0
22	6166.0
23	9842.0
24	15471.0
25	24575.0
26	34276.0
27	37686.0
28	37683.0
29	38598.0
30	42786.0
31	51676.0
32	63577.0
33	82150.0
34	129039.0
35	178120.0
36	153084.0
37	196984.0
38	304670.0
39	610532.0
40	206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.22080399604147	23.888292477066937	28.89746076638508	16.993442760506515
2	34.52755546012967	24.176100256415953	28.72628646029995	12.570057823154436
3	27.621106561928528	24.24554727160101	35.22686271751551	12.906483448954964
4	25.730439954491725	26.310892618725028	34.00939286921799	13.949274557565259
5	23.04283553100807	30.08077472661716	32.65011685558525	14.22627288678952
6	21.121320036228934	39.47063309320559	29.23127040653898	10.176776464026496
7	83.88868734282492	3.3748189170313005	10.777910222334102	1.9585835178096798
8	84.12486642431782	2.834790634771385	10.668285616274392	2.372057324636415
9	78.98327003141651	5.561733559393979	12.367541047529498	3.0874553616600156
10	35.03007149538131	36.7241443135629	18.426854820200344	9.818929370855452
11	27.1824107048107	23.29372336198578	32.18629766461583	17.337568268587688
12	26.47964837204252	21.839531491778157	33.90914632491035	17.771673811268975
13	23.403051818727004	24.641400193977802	34.32568034136145	17.629867645933746
14	17.92749771101256	29.283886268790056	33.65608673226374	19.132529287933643
15	16.611113990340595	28.595684610847456	37.924980441805424	16.86822095700652
16	21.044518646301466	26.223331136892554	36.4632367639764	16.268913452829583
17	20.89027420958981	26.42061594122423	31.836150453725438	20.852959395460523
18	20.682525462671613	27.264888290009154	34.309244054185456	17.743342193133778
19	22.360951725193175	28.053780716237124	31.528155162499598	18.0571123960701
20	23.378125917754396	28.11542913270204	31.32563838687466	17.1808065626689
21	23.180298173005497	26.506055019607555	33.497103906456296	16.81654290093065
22	21.58731098886725	23.5140090967199	34.077161704931626	20.821518209481223
23	18.727594453123263	26.85812720106811	34.00894864524026	20.40532970056836
24	18.557111162114605	26.736261756510967	35.68505507143369	19.021572009940748
25	19.77255732340246	28.061233807419033	32.5859511699132	19.580257699265303
26	18.974533626521158	30.965767606693962	30.19123842241258	19.8684603443723
27	17.99334157615603	29.876431696861555	33.59764660008243	18.532580126899983
28	17.380707352647203	28.268883837897736	35.73702927682804	18.613379532627018
29	18.32038914020449	26.76780165892977	35.211462952954214	19.70034624791153
30	19.260613868179004	27.705262326598408	33.889106887692776	19.14501691752982
31	21.036670689361575	28.024758083025464	31.650909055012207	19.287662172600758
32	21.400934351099824	28.10047359211848	32.100315645815286	18.39827641096641
33	20.84047176586435	28.366267605460006	31.19409873124696	19.599161897428683
34	19.037712147798253	27.66641740765694	32.46260497876363	20.83326546578118
35	19.09560933956234	27.645193373165416	31.827216615951098	21.431980671321146
36	21.003403249251605	28.417846945096386	30.631859250100568	19.94689055555144
37	18.87428708221352	29.905108822534988	31.217691960286377	20.00291213496512
38	19.645706698650795	29.633145031724993	29.78181198960516	20.939336280019052
39	19.5846999390426	28.629396274935154	30.526923674916894	21.258980111105352
40	20.15350406341544	27.89608120414313	31.410633241280255	20.539781491161175
41	18.018514268227374	27.868095093546163	31.700316632979682	22.413074005246777
42	19.676259436674638	28.867500326998208	30.097902028869623	21.358338207457532
43	19.670681957843144	28.58043292094541	30.496173504014056	21.252711617197388
44	19.459132628004372	29.106344752357472	30.513448880925765	20.921073738712394
45	19.196201391408216	29.64997618465897	29.32608754667437	21.827734877258447
46	20.22487604917066	29.60979859378432	29.4841325663066	20.68119279073842
47	19.50913250460882	28.818685047667703	30.87494848235814	20.797233965365336
48	19.13998237911555	28.243118847189418	31.458214565117064	21.158684208577967
49	19.478629124804726	27.908322042640567	31.333190194496062	21.279858638058645
50	18.58159283910948	28.183839625272395	31.8695166102749	21.365050925343223
51	18.337911308214935	28.566908768734528	30.711424700333907	22.38375522271663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1809.0
1	2680.5
2	3552.0
3	21848.0
4	40144.0
5	27960.0
6	15776.0
7	15711.5
8	15647.0
9	16223.0
10	16799.0
11	17323.5
12	17848.0
13	17792.0
14	17736.0
15	16662.5
16	15589.0
17	14703.5
18	13818.0
19	14018.0
20	14218.0
21	14646.5
22	15075.0
23	15254.5
24	15434.0
25	19277.0
26	26508.0
27	29896.0
28	34175.5
29	38455.0
30	45974.5
31	53494.0
32	57611.5
33	61729.0
34	68263.0
35	74797.0
36	81369.5
37	87942.0
38	94526.5
39	101111.0
40	112525.5
41	123940.0
42	136409.0
43	148878.0
44	155633.5
45	162389.0
46	165679.0
47	168969.0
48	164334.5
49	159700.0
50	143074.0
51	126448.0
52	115429.5
53	104411.0
54	95363.0
55	86315.0
56	78936.5
57	71558.0
58	66157.0
59	60756.0
60	53631.5
61	46507.0
62	40577.0
63	34647.0
64	28407.0
65	22167.0
66	17703.5
67	13240.0
68	11267.0
69	9294.0
70	7361.5
71	5429.0
72	4440.0
73	3451.0
74	2645.0
75	1469.5
76	1100.0
77	870.5
78	641.0
79	386.5
80	132.0
81	136.5
82	141.0
83	86.0
84	31.0
85	23.0
86	15.0
87	10.5
88	6.0
89	6.0
90	6.0
91	3.0
92	0.0
93	0.5
94	1.0
95	1.5
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2026005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.4861522773845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.59933125412967	18.756228894344368
2	8.7097393083715	4.265360060022123
3	3.147104135405946	2.311814132770095
4	1.673380956461475	1.6389864367196398
5	1.0959250725120884	1.3417494105067325
6	0.7217309882091694	1.060344892835815
7	0.5653264837087958	0.9689869256572331
8	0.4503826158716225	0.8822509852255455
9	0.36744850143291286	0.8097659964164736
>10	4.989040748393145	29.931679792246445
>50	1.2292920136281549	20.544053120854965
>100	0.44476033733138404	14.781371411309829
>500	0.004085990340048931	0.6730875971873483
>1k	0.0022472946870269123	0.742972649084095
>5k	0.0	0.0
>10k+	2.042995170024466E-4	1.2913476948193394
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25814	1.2741330845679057	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.871643949546028E-5	0.0	0.0	0.10074012650511721	0.0
2	9.871643949546028E-5	0.0	0.0	0.2985185130342719	0.0
3	9.871643949546028E-5	0.0	0.0	0.4613512799820336	0.0
4	9.871643949546028E-5	0.0	0.0	0.6552303671511176	0.0
5	9.871643949546028E-5	0.0	0.0	1.069592621933312	0.0
6	9.871643949546028E-5	0.0	0.0	1.636817283274227	0.0
7	9.871643949546028E-5	0.0	0.0	1.9877542256805882	0.0
8	9.871643949546028E-5	0.0	0.0	2.7353831801994564	0.0
9	9.871643949546028E-5	0.0	0.0	3.064405073037826	0.0
10	9.871643949546028E-5	0.0	0.0	3.555470001307993	0.0
11	9.871643949546028E-5	0.0	0.0	4.152753818475275	0.0
12	9.871643949546028E-5	0.0	0.0	4.604332170947258	0.0
13	9.871643949546028E-5	0.0	0.0	4.8212615467385325	0.0
14	9.871643949546028E-5	0.0	0.0	4.929800271963791	0.0
15	9.871643949546028E-5	0.0	0.0	5.057292553572178	0.0
16	9.871643949546028E-5	0.0	0.0	5.3142514455788605	0.0
17	9.871643949546028E-5	0.0	0.0	5.632266455413486	0.0
18	9.871643949546028E-5	0.0	0.0	5.987892428695882	0.0
19	9.871643949546028E-5	0.0	0.0	6.225354823902212	0.0
20	9.871643949546028E-5	0.0	0.0	6.467703682863567	0.0
21	1.480746592431904E-4	0.0	0.0	6.7573870745629945	0.0
22	1.480746592431904E-4	0.0	0.0	7.087297415356823	0.0
23	1.480746592431904E-4	0.0	0.0	7.447069479098028	0.0
24	1.480746592431904E-4	0.0	0.0	7.717799314414328	0.0
25	1.480746592431904E-4	4.935821974773014E-5	0.0	7.950128454766894	0.0
26	1.480746592431904E-4	4.935821974773014E-5	0.0	8.171055846357733	0.0
27	1.480746592431904E-4	4.935821974773014E-5	0.0	8.400226060646444	0.0
28	1.480746592431904E-4	4.935821974773014E-5	0.0	8.64583256211115	0.0
29	1.480746592431904E-4	4.935821974773014E-5	0.0	8.918141860459377	0.0
30	1.480746592431904E-4	4.935821974773014E-5	0.0	9.226285226344457	0.0
31	1.480746592431904E-4	4.935821974773014E-5	0.0	9.501358584998556	0.0
32	1.480746592431904E-4	4.935821974773014E-5	0.0	9.762315492804806	0.0
33	1.480746592431904E-4	4.935821974773014E-5	0.0	10.031564581528674	0.0
34	1.480746592431904E-4	4.935821974773014E-5	0.0	10.304466178513874	0.0
35	1.480746592431904E-4	4.935821974773014E-5	0.0	10.612806977277943	0.0
36	1.480746592431904E-4	4.935821974773014E-5	0.0	10.894543695597987	0.0
37	1.480746592431904E-4	4.935821974773014E-5	0.0	11.186349490746569	0.0
38	1.480746592431904E-4	4.935821974773014E-5	0.0	11.491679438106026	0.0
39	1.480746592431904E-4	4.935821974773014E-5	0.0	11.862409026631228	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACTA	20	7.034564E-4	45.0	36
CGTTTTT	13865	0.0	43.37721	1
CGACGGT	490	0.0	41.785713	28
CCGAACG	65	0.0	41.538464	36
TCACGAC	500	0.0	40.949997	25
CGACGTT	155	0.0	40.64516	27
CGGTCTA	505	0.0	40.544556	31
TAATGCG	50	1.0822987E-9	40.5	1
TCTCACG	510	0.0	40.14706	23
ACGGTCT	510	0.0	40.14706	30
TAGGGAT	3315	0.0	40.113125	5
GTCGTTA	45	1.929584E-8	40.0	36
AGTACGG	215	0.0	39.76744	2
CACGACG	510	0.0	39.705883	26
ATAGGGA	3095	0.0	39.47496	4
GACGGTC	520	0.0	39.375004	29
TACGGGA	950	0.0	39.31579	4
ACGACGG	525	0.0	38.999996	27
TACCCGT	35	6.2506497E-6	38.571426	27
CGAACGT	70	0.0	38.571426	37
>>END_MODULE