FastQCFastQC Report
Thu 26 May 2016
SRR1547550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547550_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3204976
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334311.0430967345777316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC351.2128294E-745.00000431
CGCGAAA207.035177E-445.033
CGTTTTT168050.043.1523361
CGACGGT6250.040.6828
TACGGGA11550.040.519484
CGGTCTA6250.040.3231
TAACGCG950.040.2631571
TCGATTG451.9301297E-840.01
TCACGAC6500.039.80769325
ACGGGTC1600.039.3755
TCGCGCA1600.039.37534
TAAGGGA40250.039.1863334
TAGGGAT47550.039.0378535
TAGGGAC36500.038.95895
CATACGA8800.038.86363618
ACGGGAG13500.038.8333325
CACGACG6500.038.7692326
TAAGTCG356.2517865E-638.571431
GCGCGAC4700.038.297879
GGGCGAT20700.038.2608687
Produced by FastQC (version 0.11.5)