##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547550_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3204976
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37069357149632	31.0	31.0	33.0	30.0	34.0
2	31.777456679862812	31.0	31.0	34.0	30.0	34.0
3	31.846280284158134	31.0	31.0	34.0	30.0	34.0
4	35.63920322648282	37.0	35.0	37.0	33.0	37.0
5	35.567262594166074	37.0	35.0	37.0	33.0	37.0
6	35.613749993759704	37.0	35.0	37.0	33.0	37.0
7	35.95802152652625	37.0	35.0	37.0	35.0	37.0
8	35.892348959867405	37.0	35.0	37.0	35.0	37.0
9	37.507060271278164	39.0	37.0	39.0	35.0	39.0
10	37.110530936893134	39.0	37.0	39.0	33.0	39.0
11	36.90142640693721	39.0	37.0	39.0	32.0	39.0
12	36.56032962493323	39.0	35.0	39.0	32.0	39.0
13	36.45039775648866	39.0	35.0	39.0	32.0	39.0
14	37.509656234555266	40.0	36.0	41.0	32.0	41.0
15	37.660819613001784	40.0	36.0	41.0	32.0	41.0
16	37.695973074369356	40.0	36.0	41.0	33.0	41.0
17	37.6020675349831	40.0	36.0	41.0	32.0	41.0
18	37.48924516127422	39.0	36.0	41.0	32.0	41.0
19	37.452970318654494	39.0	36.0	41.0	32.0	41.0
20	37.309205123532905	39.0	35.0	41.0	32.0	41.0
21	37.20396283778724	39.0	35.0	41.0	32.0	41.0
22	37.14853278152473	39.0	35.0	41.0	32.0	41.0
23	37.06904451078573	39.0	35.0	41.0	32.0	41.0
24	37.009645314036675	39.0	35.0	41.0	32.0	41.0
25	36.91576411180614	39.0	35.0	41.0	31.0	41.0
26	36.91591200682938	39.0	35.0	41.0	31.0	41.0
27	36.88120503866487	39.0	35.0	41.0	31.0	41.0
28	36.80275827338489	39.0	35.0	41.0	31.0	41.0
29	36.73406883546086	39.0	35.0	41.0	31.0	41.0
30	36.589568221415696	38.0	35.0	41.0	31.0	41.0
31	36.438894082202175	38.0	35.0	41.0	30.0	41.0
32	36.245260494930385	38.0	35.0	41.0	30.0	41.0
33	36.039245535692	38.0	35.0	41.0	29.0	41.0
34	35.84199008042494	38.0	35.0	41.0	28.0	41.0
35	35.68790873941022	38.0	35.0	41.0	27.0	41.0
36	35.646697510371375	38.0	35.0	41.0	27.0	41.0
37	35.58883436256621	38.0	35.0	41.0	27.0	41.0
38	35.45858627334495	38.0	34.0	41.0	26.0	41.0
39	35.38649150570863	38.0	34.0	40.0	26.0	41.0
40	35.299258715197865	38.0	34.0	40.0	25.0	41.0
41	35.23835966322369	38.0	34.0	40.0	25.0	41.0
42	35.24901684131176	38.0	34.0	40.0	25.0	41.0
43	35.22329246771271	38.0	34.0	40.0	25.0	41.0
44	35.10074272007029	38.0	34.0	40.0	25.0	41.0
45	35.02572187748052	38.0	34.0	40.0	24.0	41.0
46	34.98227131810035	38.0	34.0	40.0	24.0	41.0
47	34.926922697705066	38.0	34.0	40.0	24.0	41.0
48	34.81509159506967	38.0	34.0	40.0	24.0	41.0
49	34.73016428204143	37.0	34.0	40.0	24.0	41.0
50	34.642278132503954	37.0	34.0	40.0	24.0	41.0
51	34.241482931541455	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	9.0
11	10.0
12	13.0
13	24.0
14	41.0
15	88.0
16	270.0
17	531.0
18	1299.0
19	2563.0
20	4491.0
21	7275.0
22	11029.0
23	16613.0
24	24367.0
25	36510.0
26	49902.0
27	58854.0
28	62536.0
29	67656.0
30	76954.0
31	91447.0
32	111565.0
33	141753.0
34	223535.0
35	281436.0
36	237534.0
37	309515.0
38	477257.0
39	909543.0
40	354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.898631378206886	22.96934516826335	27.60803825052044	15.523985203009321
2	34.63398789881734	24.688671615637684	27.38722536455811	13.290115120986865
3	29.940692223592315	24.280899451353147	32.4749389699018	13.303469355152739
4	27.446008956073303	26.548841551387593	30.979514355177702	15.025635137361403
5	23.554560158952828	30.88450584341349	30.378792228085327	15.182141769548352
6	22.16550139533026	39.361667606871315	27.863671990055465	10.60915900774296
7	84.03810200138784	4.01903165577527	9.596140501520138	2.3467258413167524
8	83.57500961005636	3.9877989725976106	9.59055543629656	2.8466359810494684
9	77.30438543065533	7.134811617934113	11.744580926659046	3.8162220247515117
10	36.727482514689655	36.519056616960626	16.590639056267502	10.162821812082212
11	29.973141764556114	22.706098267194513	30.124905771525277	17.195854196724095
12	29.91105081598115	20.40502019359895	31.777367443625167	17.906561546794734
13	25.281811782677938	23.252030592428774	31.90588634673083	19.560271278162457
14	18.598548007847796	26.74678375126678	33.72883291481746	20.925835326067965
15	18.713619072342507	28.06654402404261	36.04875668335738	17.1710802202575
16	24.383645930577952	26.09305030677297	33.21946872613087	16.303835036518212
17	23.013744876716704	26.46631363230177	30.56674995382181	19.953191537159718
18	22.481447598983582	27.602421983815166	31.777149033253295	18.13898138394796
19	23.843080260195396	28.75129174134221	29.425337350420094	17.980290648042292
20	24.910451747532587	28.363769338678356	29.528520650388646	17.19725826340041
21	24.395533695104113	27.238300692423284	31.70245268607316	16.663712926399448
22	22.5075632391631	23.773469754531703	32.81528473224136	20.903682274063833
23	21.368334739480108	25.92331424634693	32.965738276979295	19.742612737193664
24	21.338287712606895	26.41554882158244	32.88526965568541	19.360893810125255
25	21.696293513586372	27.685511529571517	29.960099545207203	20.658095411634907
26	19.83783965932974	29.30234110957461	29.685713715172906	21.17410551592274
27	19.520801403817064	28.627203448637367	31.815121236477278	20.03687391106829
28	18.570685084693302	28.807610415803424	32.42270768954276	20.198996809960512
29	20.762183554572637	27.267536480772396	31.797617205245842	20.172662759409118
30	22.16409732865394	26.464909565625454	31.60560328688889	19.765389818831718
31	24.07453285141605	26.400509707404986	29.45563398914688	20.06932345203209
32	24.7009025964625	26.986629541063646	28.99566174598499	19.31680611648886
33	23.732720619436776	27.665043357578966	29.00265087788489	19.599585145099372
34	21.03747422757612	27.36497870810889	31.724574536595597	19.872972527719394
35	21.34758575415229	28.239899456345384	29.840597870311665	20.57191691919066
36	22.59243126937612	29.966464647473178	28.580370024611728	18.860734058538974
37	22.020164893590465	30.23763672489279	28.441305020692827	19.300893360823917
38	21.838073046412827	29.975388271238224	28.181583887055627	20.00495479529332
39	22.130212519532126	28.517717449366238	28.182239118171243	21.169830912930394
40	22.008401934991088	27.126880201287	29.95941311260989	20.905304751112023
41	19.18981608598629	27.632500212170076	30.035763138319915	23.141920563523723
42	19.72092770741497	28.387950486992725	29.404744372500762	22.48637743309154
43	21.20973760801953	26.908189016079998	29.10009310522138	22.781980270679096
44	20.923526416422465	27.988571521284406	28.809076885443137	22.27882517685
45	21.02826979047581	28.115842365122234	28.35731063196729	22.498577212434665
46	21.093418484256983	29.038626186280336	28.197902261982616	21.67005306748007
47	20.877909850182967	27.160827413372207	30.424377592843125	21.5368851436017
48	20.70268232897844	27.120234285685758	30.809060660672653	21.36802272466315
49	21.67120752230282	26.751994398710004	30.09829090763862	21.478507171348554
50	19.493749719186663	27.128377872408404	30.742383094288385	22.63548931411655
51	19.20273349940842	26.72640918371932	30.722507750448052	23.348349566424208
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2131.0
1	3978.0
2	5825.0
3	30493.0
4	55161.0
5	38405.0
6	21649.0
7	20792.5
8	19936.0
9	20143.5
10	20351.0
11	20198.5
12	20046.0
13	19593.5
14	19141.0
15	18242.0
16	17343.0
17	17262.5
18	17182.0
19	16983.0
20	16784.0
21	17654.0
22	18524.0
23	19373.0
24	20222.0
25	25483.0
26	36237.0
27	41730.0
28	50618.0
29	59506.0
30	68214.0
31	76922.0
32	85399.5
33	93877.0
34	104355.0
35	114833.0
36	118066.5
37	121300.0
38	130031.0
39	138762.0
40	152149.5
41	165537.0
42	178232.5
43	190928.0
44	198752.0
45	206576.0
46	221432.0
47	236288.0
48	244277.0
49	252266.0
50	241013.0
51	229760.0
52	213777.0
53	197794.0
54	185060.5
55	172327.0
56	165335.0
57	158343.0
58	149672.0
59	141001.0
60	127597.0
61	114193.0
62	97752.0
63	81311.0
64	66869.0
65	52427.0
66	41483.5
67	30540.0
68	24141.5
69	17743.0
70	14151.0
71	10559.0
72	9088.5
73	7618.0
74	5609.0
75	2800.0
76	2000.0
77	1622.5
78	1245.0
79	918.5
80	592.0
81	395.5
82	199.0
83	144.5
84	90.0
85	59.0
86	28.0
87	24.5
88	21.0
89	13.5
90	6.0
91	6.5
92	7.0
93	6.5
94	6.0
95	3.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3204976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.784770422952057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86300367766836	15.975798854588566
2	8.62429221965292	3.5850786769187506
3	3.164581510139635	1.973253005189137
4	1.62308434491398	1.3494174194449844
5	1.0163043024838783	1.0561825803492917
6	0.7029693205813168	0.8766633565596753
7	0.5470442147345737	0.7959131890123541
8	0.40738011376044325	0.6773841711509524
9	0.31475428342912415	0.5887885968643615
>10	4.252733271615308	21.763248956239163
>50	1.663256272544876	24.556297124319666
>100	0.8114710011852263	23.665799951696563
>500	0.005019007009749237	0.7100008132607906
>1k	0.003954369159196369	1.3693595382382426
>5k	0.0	0.0
>10k+	1.5209112150755264E-4	1.0568137661674752
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33431	1.0430967345777316	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1201481695962775E-5	0.0	0.0	0.08705213393173615	0.0
2	3.1201481695962775E-5	0.0	0.0	0.2461796905811463	0.0
3	3.1201481695962775E-5	0.0	0.0	0.3798780396483468	0.0
4	3.1201481695962775E-5	0.0	0.0	0.5416889237236098	0.0
5	3.1201481695962775E-5	0.0	0.0	0.9305217886186979	0.0
6	3.1201481695962775E-5	0.0	0.0	1.4289654587117033	0.0
7	3.1201481695962775E-5	0.0	0.0	1.7558009794769134	0.0
8	3.1201481695962775E-5	0.0	0.0	2.4431384197572776	0.0
9	3.1201481695962775E-5	0.0	0.0	2.8027042948215524	0.0
10	3.1201481695962775E-5	0.0	0.0	3.30267683751766	0.0
11	3.1201481695962775E-5	0.0	0.0	3.8454890145823244	0.0
12	3.1201481695962775E-5	0.0	0.0	4.278846393857552	0.0
13	3.1201481695962775E-5	0.0	0.0	4.474448482609542	0.0
14	3.1201481695962775E-5	0.0	0.0	4.583466459655236	0.0
15	3.1201481695962775E-5	0.0	0.0	4.708428393847567	0.0
16	3.1201481695962775E-5	0.0	0.0	4.921815327166256	0.0
17	3.1201481695962775E-5	0.0	0.0	5.175888992616481	0.0
18	3.1201481695962775E-5	0.0	0.0	5.4713670242772485	0.0
19	3.1201481695962775E-5	0.0	0.0	5.674301461227791	0.0
20	3.1201481695962775E-5	0.0	0.0	5.899607360554338	0.0
21	3.1201481695962775E-5	0.0	0.0	6.146317476324316	0.0
22	3.1201481695962775E-5	0.0	0.0	6.436460054615074	0.0
23	3.1201481695962775E-5	0.0	0.0	6.717023778025171	0.0
24	3.1201481695962775E-5	0.0	0.0	6.936619806201357	0.0
25	3.1201481695962775E-5	0.0	0.0	7.1381813779572765	0.0
26	3.1201481695962775E-5	0.0	0.0	7.333783466709267	0.0
27	6.240296339192555E-5	0.0	0.0	7.539588439975837	0.0
28	6.240296339192555E-5	0.0	0.0	7.763739884479635	0.0
29	6.240296339192555E-5	0.0	0.0	8.010637209139787	0.0
30	6.240296339192555E-5	0.0	0.0	8.296005960731064	0.0
31	6.240296339192555E-5	0.0	0.0	8.55160849878439	0.0
32	6.240296339192555E-5	0.0	0.0	8.793669593781669	0.0
33	6.240296339192555E-5	0.0	0.0	9.037072352491876	0.0
34	6.240296339192555E-5	0.0	0.0	9.287027422358232	0.0
35	6.240296339192555E-5	0.0	0.0	9.559135544228724	0.0
36	6.240296339192555E-5	0.0	0.0	9.824816160869847	0.0
37	6.240296339192555E-5	0.0	0.0	10.104693451682634	0.0
38	6.240296339192555E-5	0.0	0.0	10.439173335463355	0.0
39	6.240296339192555E-5	0.0	0.0	10.881173525168363	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	35	1.2128294E-7	45.000004	31
CGCGAAA	20	7.035177E-4	45.0	33
CGTTTTT	16805	0.0	43.152336	1
CGACGGT	625	0.0	40.68	28
TACGGGA	1155	0.0	40.51948	4
CGGTCTA	625	0.0	40.32	31
TAACGCG	95	0.0	40.263157	1
TCGATTG	45	1.9301297E-8	40.0	1
TCACGAC	650	0.0	39.807693	25
ACGGGTC	160	0.0	39.375	5
TCGCGCA	160	0.0	39.375	34
TAAGGGA	4025	0.0	39.186333	4
TAGGGAT	4755	0.0	39.037853	5
TAGGGAC	3650	0.0	38.9589	5
CATACGA	880	0.0	38.863636	18
ACGGGAG	1350	0.0	38.833332	5
CACGACG	650	0.0	38.76923	26
TAAGTCG	35	6.2517865E-6	38.57143	1
GCGCGAC	470	0.0	38.29787	9
GGGCGAT	2070	0.0	38.260868	7
>>END_MODULE