FastQCFastQC Report
Thu 26 May 2016
SRR1547549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547549_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3426163
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202430.5908358709144894No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGC207.0352445E-445.0000044
CCCGTTA253.8924303E-545.024
CGTACCG253.8924303E-545.044
TTACGCG800.042.1875041
CGTTTTT101350.041.7143551
TAATCGG650.041.5384642
TACGGGA15550.040.6591644
TTTCGCG501.0822987E-940.51
GCGCTAA501.0822987E-940.520
CTCACGA9700.040.36082524
GGGCGAT29500.040.1949127
GTATCGA451.9303116E-840.043
CGACGGT9850.039.74619328
CGAACTA800.039.37500440
TACGTAG1600.039.3750041
CACGACG9900.039.31818426
CGGTCTA9850.039.2893431
AGGGATC42350.038.9433256
CGGGAAT7050.038.9361726
TAAGGGA47250.038.619054
Produced by FastQC (version 0.11.5)