##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547549_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3426163
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.481119549770398	31.0	31.0	33.0	30.0	34.0
2	31.911130030882944	31.0	31.0	34.0	30.0	34.0
3	32.04257824277479	33.0	31.0	34.0	30.0	34.0
4	35.765356172488	37.0	35.0	37.0	35.0	37.0
5	35.69105964894256	37.0	35.0	37.0	33.0	37.0
6	35.72830335275934	37.0	35.0	37.0	33.0	37.0
7	36.029154479807296	37.0	35.0	37.0	35.0	37.0
8	35.97797769691635	37.0	35.0	37.0	35.0	37.0
9	37.62465737911477	39.0	38.0	39.0	35.0	39.0
10	37.23398448935442	39.0	37.0	39.0	34.0	39.0
11	37.019752708788225	39.0	37.0	39.0	33.0	39.0
12	36.66126772135476	39.0	35.0	39.0	33.0	39.0
13	36.52231111012523	39.0	35.0	39.0	32.0	39.0
14	37.547799973322924	40.0	36.0	41.0	32.0	41.0
15	37.762045179987055	40.0	36.0	41.0	33.0	41.0
16	37.832238279381336	40.0	36.0	41.0	33.0	41.0
17	37.76336268881545	40.0	36.0	41.0	33.0	41.0
18	37.66935052418697	39.0	36.0	41.0	33.0	41.0
19	37.666226913313814	39.0	36.0	41.0	33.0	41.0
20	37.55664777186608	39.0	35.0	41.0	33.0	41.0
21	37.43604463652196	39.0	35.0	41.0	32.0	41.0
22	37.40566371185492	39.0	35.0	41.0	32.0	41.0
23	37.33655404019015	39.0	35.0	41.0	32.0	41.0
24	37.275265654319426	39.0	35.0	41.0	32.0	41.0
25	37.176735899605475	39.0	35.0	41.0	32.0	41.0
26	37.19570230604907	39.0	35.0	41.0	32.0	41.0
27	37.1645867403273	39.0	35.0	41.0	32.0	41.0
28	37.10314424620195	39.0	35.0	41.0	32.0	41.0
29	37.05985617146645	39.0	35.0	41.0	32.0	41.0
30	36.97120802483711	39.0	35.0	41.0	31.0	41.0
31	36.82883534729667	39.0	35.0	41.0	31.0	41.0
32	36.72596312551388	39.0	35.0	41.0	31.0	41.0
33	36.62258363072627	39.0	35.0	41.0	30.0	41.0
34	36.51126289087822	39.0	35.0	41.0	30.0	41.0
35	36.40190907437854	39.0	35.0	41.0	30.0	41.0
36	36.32805181773313	39.0	35.0	41.0	30.0	41.0
37	36.283422592562	39.0	35.0	41.0	30.0	41.0
38	36.1469480582214	39.0	35.0	41.0	30.0	41.0
39	36.08341488714927	39.0	35.0	41.0	29.0	41.0
40	35.99243614504039	39.0	35.0	41.0	29.0	41.0
41	35.926735826637554	38.0	35.0	41.0	29.0	41.0
42	35.91485694054836	38.0	35.0	41.0	29.0	41.0
43	35.894249339567324	38.0	35.0	41.0	29.0	41.0
44	35.779073266508334	38.0	35.0	40.0	28.0	41.0
45	35.723351165720956	38.0	35.0	40.0	28.0	41.0
46	35.70373943096111	38.0	35.0	40.0	28.0	41.0
47	35.64312264185913	38.0	35.0	40.0	28.0	41.0
48	35.550500953982635	38.0	34.0	40.0	28.0	41.0
49	35.48192394816009	38.0	34.0	40.0	28.0	41.0
50	35.388674444269	38.0	34.0	40.0	27.0	41.0
51	34.98779275825464	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	6.0
11	10.0
12	19.0
13	23.0
14	35.0
15	109.0
16	240.0
17	546.0
18	1087.0
19	2045.0
20	3634.0
21	5898.0
22	9178.0
23	13571.0
24	20458.0
25	29741.0
26	40265.0
27	47119.0
28	52239.0
29	58914.0
30	71075.0
31	86525.0
32	110370.0
33	144081.0
34	237399.0
35	316634.0
36	248184.0
37	329360.0
38	524957.0
39	1072079.0
40	358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.92742843816829	24.15028123297111	28.89126991331119	13.031020415549408
2	32.14496800064679	24.98013083440572	29.53747384464779	13.337427320299705
3	30.184028022017635	24.05326308176231	31.96689707991126	13.795811816308797
4	26.924375752116873	27.300627553330063	30.791529766680686	14.983466927872374
5	24.884221795635526	31.72788334939114	28.606549075452627	14.781345779520706
6	22.676591860924304	40.570544950721846	26.05608664853365	10.6967765398202
7	87.32450849536347	3.953139415725405	6.699973118616949	2.0223789702941746
8	87.7059847999059	3.3371442047561657	6.6638394028538634	2.2930315924840703
9	82.00876607446872	6.521289267323242	8.439003048016104	3.030941610191926
10	38.745412871483346	36.893866403904305	14.256618847381167	10.104101877231177
11	31.056461703660915	23.928341996571675	28.27072150390977	16.744474795857638
12	29.804448883488615	22.025192613427908	30.362186504261473	17.808171998822004
13	26.009883359314777	25.472635131486737	30.054612112733693	18.462869396464793
14	18.603609927490314	30.109659114291993	32.30304571031793	18.983685247899764
15	18.446553768749475	28.875216970120803	35.757288838855594	16.920940422274132
16	24.070308388713553	26.716066923844544	32.879900927072065	16.333723760369836
17	23.82808406955536	25.76911839862844	28.693089032833523	21.70970849898268
18	23.62724715665892	27.0557472017531	31.193991646048364	18.123013995539615
19	25.281313235826786	28.14165584065907	28.20531305720131	18.37171786631284
20	25.814737944458564	29.249104610609596	27.52916309002228	17.406994354909557
21	24.73951180956656	26.157336939310827	31.845507642222508	17.257643608900104
22	23.49861930094978	23.066590818942355	32.36390095859421	21.07088892151366
23	20.593970572912028	26.821870413053905	32.05962471721281	20.524534296821255
24	20.34961559038493	27.54544953056816	32.91472122021048	19.19021365883643
25	21.07833164972011	28.86949628491114	30.215520977840228	19.836651087528526
26	20.158264507555536	31.381840268545307	27.912244688883746	20.54765053501541
27	19.335886821496818	29.92528376495806	31.81874300784872	18.920086405696402
28	18.504519487251482	28.588365468893333	33.09039879305217	19.81671625080301
29	19.721215832404937	27.2515931086758	32.736912983999886	20.290278074919378
30	21.140821379484866	27.267441741680127	32.10060350310245	19.49113337573256
31	23.31999382399495	26.987624348286992	29.8059374291299	19.886444398588157
32	24.0086067125236	28.43157199467743	28.64422971119588	18.915591581603096
33	23.653048614441285	27.49694045496376	28.874049483343324	19.975961447251635
34	19.958244835403335	28.4509230880142	30.910438294967285	20.680393781615177
35	20.986742312026603	28.191682649074195	29.90800496065132	20.913570078247883
36	23.80312903968667	27.842866787131843	29.00962388537848	19.344380287803002
37	21.13440020220871	30.152681002042225	28.904579262574487	19.808339533174575
38	21.282495899932375	29.662248993991234	27.54565384075422	21.50960126532217
39	20.257325760624934	28.95875064904968	28.630745238915956	22.153178351409434
40	22.035349748392004	27.77217546275527	29.13442822189137	21.058046566961348
41	19.959762568214064	27.316125940301145	29.534788625059576	23.18932286642521
42	21.021796102520515	27.707525882452178	29.34191980941946	21.92875820560785
43	20.981401060019618	27.398667255469167	29.58744227872404	22.032489405787175
44	20.803767946825648	27.947444415224844	29.752845967923886	21.495941670025623
45	20.414644603890707	29.051128040317987	28.261118925164975	22.27310843062633
46	21.538671686081486	28.60313417662849	28.49050088977086	21.367693247519163
47	20.535450298190717	28.76818761979509	29.558050799100922	21.13831128291328
48	20.55976321033179	28.761299447807943	29.99428223350728	20.684655108352988
49	21.468680853771403	27.17322555873728	30.081259998429733	21.27683358906158
50	19.76762343180987	28.856303684325585	29.546959674714834	21.82911320914971
51	19.52414406436588	29.580145486364778	28.714162169167086	22.18154828010226
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2101.0
1	4103.5
2	6106.0
3	19647.5
4	33189.0
5	23269.5
6	13350.0
7	13100.5
8	12851.0
9	13575.0
10	14299.0
11	14532.0
12	14765.0
13	14696.5
14	14628.0
15	13644.5
16	12661.0
17	12503.5
18	12346.0
19	13612.5
20	14879.0
21	17371.5
22	19864.0
23	20826.5
24	21789.0
25	26341.0
26	38812.0
27	46731.0
28	55979.0
29	65227.0
30	73212.5
31	81198.0
32	91406.5
33	101615.0
34	118912.5
35	136210.0
36	142756.5
37	149303.0
38	160516.0
39	171729.0
40	192003.0
41	212277.0
42	234984.0
43	257691.0
44	265091.0
45	272491.0
46	277651.0
47	282811.0
48	279042.5
49	275274.0
50	251089.0
51	226904.0
52	210504.5
53	194105.0
54	180372.5
55	166640.0
56	159236.5
57	151833.0
58	138333.5
59	124834.0
60	110949.0
61	97064.0
62	80893.5
63	64723.0
64	55423.0
65	46123.0
66	39191.0
67	32259.0
68	25711.0
69	19163.0
70	15289.0
71	11415.0
72	8946.0
73	6477.0
74	5135.0
75	3079.0
76	2365.0
77	1767.5
78	1170.0
79	861.5
80	553.0
81	389.0
82	225.0
83	142.5
84	60.0
85	89.0
86	118.0
87	69.0
88	20.0
89	19.0
90	18.0
91	14.0
92	10.0
93	8.5
94	7.0
95	5.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3426163.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.870300895576634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.25987380058203	12.261374443695148
2	9.269176445039347	2.9420923847393166
3	3.0822216774785907	1.4674735634536256
4	1.6256978648027935	1.0320125711886718
5	0.9411895101275498	0.7468480362742294
6	0.6515266488132099	0.6203954374891396
7	0.4550997796462932	0.5055799308348107
8	0.36724857031123925	0.46626762514477593
9	0.2958733269036715	0.4226038852442919
>10	2.8944650083855956	10.688584860342477
>50	1.2454843951863717	14.508542238912645
>100	1.9024366723951334	52.40935373864212
>500	0.006533086759427121	0.663772501059001
>1k	0.002986553947166684	0.6654314139013853
>5k	0.0	0.0
>10k+	1.8665962169791775E-4	0.5996673690782951
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20243	0.5908358709144894	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050727300481617485	0.0
2	0.0	0.0	0.0	0.1543417519831952	0.0
3	0.0	0.0	0.0	0.2268718680342996	0.0
4	0.0	0.0	0.0	0.3333174749712725	0.0
5	0.0	0.0	0.0	0.5680115044147053	0.0
6	0.0	0.0	0.0	0.8200135253343172	0.0
7	0.0	0.0	0.0	0.9871684447003835	0.0
8	0.0	0.0	0.0	1.3429600401381954	0.0
9	0.0	0.0	0.0	1.4871738443267293	0.0
10	0.0	0.0	0.0	1.7505004869879222	0.0
11	0.0	0.0	0.0	2.1125381366852656	0.0
12	0.0	0.0	0.0	2.4109477570098097	0.0
13	0.0	0.0	0.0	2.5298854724658457	0.0
14	0.0	0.0	0.0	2.5883181856788484	0.0
15	0.0	0.0	0.0	2.669195832188953	0.0
16	0.0	0.0	0.0	2.836234002877271	0.0
17	0.0	0.0	0.0	3.0376546591624507	0.0
18	0.0	0.0	0.0	3.269371597323303	0.0
19	0.0	0.0	0.0	3.4213783757515333	0.0
20	0.0	0.0	0.0	3.5958009003074283	0.0
21	0.0	0.0	0.0	3.7964335030178074	0.0
22	0.0	0.0	0.0	4.020532589955586	0.0
23	0.0	0.0	0.0	4.234328606082081	0.0
24	0.0	0.0	0.0	4.408634381960228	0.0
25	0.0	0.0	0.0	4.567208273511797	0.0
26	0.0	0.0	0.0	4.716092024810261	0.0
27	0.0	0.0	0.0	4.891098292754898	0.0
28	0.0	0.0	0.0	5.05769865590166	0.0
29	0.0	0.0	0.0	5.261249975555746	0.0
30	0.0	0.0	0.0	5.487508913031867	0.0
31	0.0	0.0	0.0	5.682479204871456	0.0
32	0.0	0.0	0.0	5.869656522471348	0.0
33	0.0	0.0	0.0	6.062846396975275	0.0
34	0.0	0.0	0.0	6.271651407128032	0.0
35	0.0	0.0	0.0	6.503485093966632	0.0
36	0.0	0.0	0.0	6.707328285315088	0.0
37	0.0	0.0	0.0	6.929384270392273	0.0
38	2.9187169437064145E-5	0.0	0.0	7.182874836953174	0.0
39	2.9187169437064145E-5	0.0	0.0	7.636268326988529	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGC	20	7.0352445E-4	45.000004	4
CCCGTTA	25	3.8924303E-5	45.0	24
CGTACCG	25	3.8924303E-5	45.0	44
TTACGCG	80	0.0	42.187504	1
CGTTTTT	10135	0.0	41.714355	1
TAATCGG	65	0.0	41.538464	2
TACGGGA	1555	0.0	40.659164	4
TTTCGCG	50	1.0822987E-9	40.5	1
GCGCTAA	50	1.0822987E-9	40.5	20
CTCACGA	970	0.0	40.360825	24
GGGCGAT	2950	0.0	40.194912	7
GTATCGA	45	1.9303116E-8	40.0	43
CGACGGT	985	0.0	39.746193	28
CGAACTA	80	0.0	39.375004	40
TACGTAG	160	0.0	39.375004	1
CACGACG	990	0.0	39.318184	26
CGGTCTA	985	0.0	39.28934	31
AGGGATC	4235	0.0	38.943325	6
CGGGAAT	705	0.0	38.936172	6
TAAGGGA	4725	0.0	38.61905	4
>>END_MODULE