FastQCFastQC Report
Thu 26 May 2016
SRR1547548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547548_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4439903
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT821751.8508287230599405No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT75220.16941811566604045No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT65650.1478635907135809TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT59700.1344623970388542No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC55490.12498020790093839TruSeq Adapter, Index 19 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC54000.12162427872861187No Hit
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG46540.10482210985239993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAC406.8230293E-945.0000044
CGTTTTT346050.043.7841341
CGACGGT9600.042.65625428
TACGGGA23250.041.032264
CACGACG10200.040.1470626
ACGGGAT29600.039.983115
GGCGATA7000.039.8571438
TCACGAC10550.039.66824325
TACGACG1250.039.61
CGTTAGG6600.039.2045482
AGGGATC62650.039.074226
CGAGGGA32350.039.018554
TTGCGAG3750.039.01
CTCACGA10850.038.778824
TAGGGAT82150.038.7279365
TAACGGG12050.038.651453
GGCACCG7600.038.4868438
CGGGATT27850.038.3752256
GACGGTC10750.038.09302529
CGGTCTA10850.037.9493131
Produced by FastQC (version 0.11.5)